N-Cadherin Antibody (13A9) [DyLight 550]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
13A9
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 550

N-Cadherin Antibody (13A9) [DyLight 550] Summary

Immunogen
Cytoplasmic domain of human N Cadherin [Swiss-Prot# P19022]
Localization
Cell membrane; Single-pass type I membrane protein.
Marker
Mesenchymal Cells Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CDH2
Purity
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This N Cadherin (13A9) antibody is useful for Immunofluorescence/Immunocytochemistry, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western Blot, where a band is observed at ~140 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for N-Cadherin Antibody (13A9) [DyLight 550]

  • cadherin 2, N-cadherin (neuronal)
  • cadherin 2, type 1, N-cadherin (neuronal)
  • Cadherin-2
  • CD325 antigen
  • CD325
  • CDH2
  • CDHNcalcium-dependent adhesion protein, neuronal
  • CDw325
  • NCadherin
  • N-Cadherin
  • NCADN-cadherin 1
  • Neural cadherin
  • neural-cadherin

Background

N Cadherin, also known as Neuronal Cadherin or Cadherin 2, is a classical cadherin from the cadherin superfamily. It is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. N Cadherin functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. N Cadherin has also been identified as a potential prognostic marker of a number of tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for N-Cadherin Antibody (NBP1-48309R) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-48309B
DyLight 350 Labeled NBP1-48309UV
DyLight 405 Labeled NBP1-48309V
DyLight 488 Labeled NBP1-48309G
DyLight 650 Labeled NBP1-48309C
DyLight 680 Labeled NBP1-48309FR
DyLight 755 Labeled NBP1-48309IR
FITC Labeled NBP1-48309F
HRP Labeled NBP1-48309H

Additional N-Cadherin Products

Bioinformatics Tool for N-Cadherin Antibody (NBP1-48309R)

Discover related pathways, diseases and genes to N-Cadherin Antibody (NBP1-48309R). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for N-Cadherin Antibody (NBP1-48309R)

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Pathways for N-Cadherin Antibody (NBP1-48309R)

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PTMs for N-Cadherin Antibody (NBP1-48309R)

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Research Areas for N-Cadherin Antibody (NBP1-48309R)

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Blogs on N-Cadherin.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

MMP24 (Matrix metalloproteinase-24, matrix metalloproteinase-25, MT5-MMP)
MMP24 is an extracellular matrix (ECM) degradative peptidase enzyme that is a member of the large family of matrix metalloproteinases (MMP). Each MMP has a different substrate specificity, and the aberrant or derailed expression of these is strongly c...  Read full blog post.

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Bioinformatics

Gene Symbol CDH2
Entrez