Twist-1 Antibody (10E4E6)

Images

 
Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.
Immunocytochemistry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of Hela cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TWIST1 mouse mAb with DAB staining.
Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of Hela cells using TWIST1 mouse mAb (green) and negative control (red).
Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mAb against human TWIST1 recombinant protein. (Expected MW is 31.9 kDa)
Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Western blot analysis using TWIST1 mAb against HEK293 (1) and TWIST1 (AA: 9-74)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of paraffin-embedded cervical cancer tissues using TWIST1 mouse mAb with DAB staining.
ELISA: Twist-1 Antibody (10E4E6) [NBP2-37364] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, ICC
Clone
10E4E6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Twist-1 Antibody (10E4E6) Summary

Immunogen
Purified recombinant fragment of human TWIST1 (AA: 9-74) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TWIST1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • CyTOF-ready
  • Immunocytochemistry
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Twist-1 Antibody (10E4E6)

  • acrocephalosyndactyly 3
  • ACS3
  • B-HLH DNA binding protein
  • BHLHA38
  • bHLHa38BPES3
  • BPES3
  • Class A basic helix-loop-helix protein 38
  • CRS1
  • H-twist
  • H-twistblepharophimosis, epicanthus inversus and ptosis 3
  • SCSBPES2
  • twist homolog 1 (Drosophila)
  • TWIST homolog of drosophila
  • TWIST
  • Twist1
  • Twist-1
  • twist-related protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-37578
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
NBP1-83976
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-03886
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-P
H00117581-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP2-00932
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB300-223
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-48309
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NBP2-27293
Species: Hu
Applications: WB, Simple Western, IHC-P
MAB766
Species: Hu
Applications: WB, Flow, CyTOF-ready
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-82991
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-20486
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-03644
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-05987
Species: Hu, Mu, Rt
Applications: WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP
NBP2-37364
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, ICC

Publications for Twist-1 Antibody (NBP2-37364) (0)

There are no publications for Twist-1 Antibody (NBP2-37364).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Twist-1 Antibody (NBP2-37364) (0)

There are no reviews for Twist-1 Antibody (NBP2-37364). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Twist-1 Antibody (NBP2-37364) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Twist-1 Products

Bioinformatics Tool for Twist-1 Antibody (NBP2-37364)

Discover related pathways, diseases and genes to Twist-1 Antibody (NBP2-37364). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Twist-1 Antibody (NBP2-37364)

Discover more about diseases related to Twist-1 Antibody (NBP2-37364).
 

Pathways for Twist-1 Antibody (NBP2-37364)

View related products by pathway.

PTMs for Twist-1 Antibody (NBP2-37364)

Learn more about PTMs related to Twist-1 Antibody (NBP2-37364).
 

Research Areas for Twist-1 Antibody (NBP2-37364)

Find related products by research area.

Blogs on Twist-1.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Twist-1 Antibody (10E4E6) and receive a gift card or discount.

Bioinformatics

Gene Symbol TWIST1
Entrez