Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of Hela cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TWIST1 mouse mAb with DAB staining.
Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on SH-SY5Y cells with Twist-1 Antibody (10E4E6) NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed ...read more
Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mAb against human TWIST1 recombinant protein. (Expected MW is 31.9 kDa)
Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Western blot analysis using TWIST1 mAb against HEK293 (1) and TWIST1 (AA: 9-74)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of paraffin-embedded cervical cancer tissues using TWIST1 mouse mAb with DAB staining.
Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of Hela cells using TWIST1 mouse mAb (green) and negative control (red).
Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Flow (Intracellular): Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed ...read more
This antibody is Cytof ready. Use in Knockdown Validated reported in scientific literature (PMID: 31113881).
Theoretical MW
21 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP2-37364 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified
Alternate Names for Twist-1 Antibody (10E4E6)
acrocephalosyndactyly 3
ACS3
B-HLH DNA binding protein
BHLHA38
bHLHa38BPES3
BPES3
Class A basic helix-loop-helix protein 38
CRS1
H-twist
H-twistblepharophimosis, epicanthus inversus and ptosis 3
SCSBPES2
twist homolog 1 (Drosophila)
TWIST homolog of drosophila
TWIST
Twist1
Twist-1
twist-related protein 1
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Bioinformatics Tool for Twist-1 Antibody (NBP2-37364)
Discover related pathways, diseases and genes to Twist-1 Antibody (NBP2-37364). Need help?
Read the Bioinformatics Tool Guide for instructions on using this tool.
Diseases for Twist-1 Antibody (NBP2-37364)
Discover more about diseases related to Twist-1 Antibody (NBP2-37364).
Autophagy and Metastasis By Christina Towers, PhD The majority of cancer patients die from metastatic disease at secondary sites. The threshold to undergo metastasis is high. Only a minority of cancer cells acquire invasive phenotypes... Read full blog post.
Epithelial-Mesenchymal Transition (EMT) Markers Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a... Read full blog post.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.