Twist-1 Antibody (10E4E6)

Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.

Immunocytochemistry/Immunofluorescence: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of Hela cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TWIST1 mouse mAb with DAB staining.

Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on SH-SY5Y cells with Twist-1 Antibody (10E4E6) NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.

Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mAb against human TWIST1 recombinant protein. (Expected MW is 31.9 kDa)

Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Western blot analysis using TWIST1 mAb against HEK293 (1) and TWIST1 (AA: 9-74)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of paraffin-embedded cervical cancer tissues using TWIST1 mouse mAb with DAB staining.

Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of Hela cells using TWIST1 mouse mAb (green) and negative control (red).

Flow Cytometry: Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550.

Flow (Intracellular): Twist-1 Antibody (10E4E6) [NBP2-37364] - An intracellular stain was performed on HeLa cells with Twist-1 Antibody (10E4E6)NBP2-37364R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550. Image from the DyLight 550 version of this antibody.

ELISA: Twist-1 Antibody (10E4E6) [NBP2-37364] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-P, CyTOF-ready, KD
• Clone: 10E4E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Twist-1 Antibody (10E4E6) Summary

• Immunogen: Purified recombinant fragment of human TWIST1 (AA: 9-74) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TWIST1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • CyTOF-ready
  • Flow (Intracellular)
  • Knockdown Validated
• Application Notes: This antibody is Cytof ready. Use in Knockdown Validated reported in scientific literature (PMID: 31113881).
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Twist-1 Antibody (10E4E6)

• acrocephalosyndactyly 3
• ACS3
• B-HLH DNA binding protein
• BHLHA38
• bHLHa38BPES3
• BPES3
• Class A basic helix-loop-helix protein 38
• CRS1
• H-twist
• H-twistblepharophimosis, epicanthus inversus and ptosis 3
• SCSBPES2
• twist homolog 1 (Drosophila)
• TWIST homolog of drosophila
• TWIST
• Twist1
• Twist-1
• twist-related protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Twist-1 Antibody (NBP2-37364)(4)

Publications using NBP2-37364

• Li J, Pu T, Yin L et al. MAOA-mediated reprogramming of stromal fibroblasts promotes prostate tumorigenesis and cancer stemness Oncogene Feb 17 2020 [PMID: 32066880] (WB, IP, IHC)
• Xu G, Yamamoto N, Nojima T, et al. Novel replisome-associated proteins at cellular replication forks in EBV-transformed B lymphocytes PLoS Pathog. Dec 1 2019 [PMID: 31841561] (WB)
• Lopez JA Muscle development and regeneration controlled by AUF1-mediated stage-specific degradation of fate-determining checkpoint mRNAs Proc. Natl. Acad. Sci. U.S.A. May 21 2019 [PMID: 31113881] (IHC-P, WB, KD, Mouse)
• Yim SH, Clish CB, Gladyshev VN Defining Reprogramming Checkpoints from Single-Cell Analyses of Induced Pluripotency Cell Rep May 7 2019 [PMID: 31067459] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: TWIST1