Cadherin-11 Antibody (283416)

Western blot shows lysates of PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cadherin‑11 Monoclonal Antibody (Catalog # MAB1790) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cadherin‑11 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cadherin‑11 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Cadherin‑11 Monoclonal Antibody (Catalog # MAB1790) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of PC‑3 human prostate cancer cell line, loaded at 0.2 mg/mL. Specific bands were detected for Cadherin‑11 at approximately 114 & 137 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Cadherin‑11 Monoclonal Antibody (Catalog # MAB1790). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clone: 283416
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cadherin-11 Antibody (283416) Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cadherin‑11
  Phe23-Thr617
  Accession # AAA35622
• Specificity: Detects human Cadherin-11 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Cadherin‑4, rhCadherin‑8, rhCadherin‑12, rhCadherin‑13, rhCadherin‑17, rhE-Cadherin, rhN-Cadherin, rhP-Cadherin, or rhVE-Cadherin.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH11
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cadherin-11 Antibody (283416)

• CAD11OSF-4
• cadherin 11, type 2, OB-cadherin (osteoblast)
• Cadherin11
• Cadherin-11
• CDH11
• CDHOB
• OB
• OB-Cadherin
• Osteoblast cadherin

Background

The cadherin superfamily comprises a large number of membrane glycoproteins with one or more cadherin repeats, which are involved in Ca²⁺ dependent cell-cell adhesion. The family can be subdivided into several major subgroups, including the type I and type II classical cadherins, desmosomal cadherins, protocadherins, seven transmembrane (Flamingo) cadherins, FAT-family cadherins, T-cadherin and other unclassified cadherins (1). Cadherin-11, also known as OB-cadherin, is a type II classical cadherin. Classical cadherins are type I transmembrane proteins with an N-terminal extracellular domain containing five tandem cadherin repeats and a C-terminal cytoplasmic domain with a characteristic sequence for binding to catenins. Type I cadherins (E-, N-, P-, R-, M-, and EP-cadherin) differ from type II cadherins (cadherin-5 to -12, -18 to -20 and -22) by the presence of the HAV tripeptide motif in the most N-terminal cadherin repeat (2). Classic cadherins mediate cell-cell adhesion preferentially via homotypic interactions and form adherens juctions that have beta -catenin and p120 (ctn) at the cytoplasmic side of the junction (3, 4). Homotypic cadherin interactions also transduce outside-in and inside-out cell signals. Cadherin signaling induces various cellular processes including cell motility, actin cytoskeleton reorganization, proliferation, and differentiation (3, 4). Cadherin-11 is expressed in a variety or normal tissues of mesodermal origin including areas of the kidney and brain, in normal osteoblasts, and in tumors of the stomach, kidney, colon, breast, and bone (osteosarcoma) (5, 6). It is also differentially expressed in the embryonic brain and may be important in regulating neural development. Human Cadherin-11 exhibits a unique mRNA splice site allowing for two forms of the protein to be expressed, a full-length 796 amino acid (aa) protein and a COOH terminus-truncated variant of 693 aa. The truncated variant has a unique cytoplasmic region due to a frameshift event (3). The full-length human and mouse Cadherin-11 share 97% homology at the aa sequence level.

1. Angst, B.D. et al. (2001) J. Cell Sci. 113:629.
2. Gessner, R. and R. Tauber (2000) Ann. N.Y. Acad. Sci. 915:136.
3. Feltes, C.M. et al. (2002) Cancer Research. 62:6688.
4. Wheelock, J.J. and K.R. Johnson (2003) Annu. Rev. Cell Dev. Biol. 19:207.
5. Hoffmann, I. and R. Balling (1995) Dev. Biol. 169:337.
6. Pishvaian, M.J. et. al. (1999) Cancer Research 59:947.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cadherin-11 Antibody (MAB1790)(7)

Publications using MAB1790

• J Feldt, M Schicht, J Welss, K Gelse, S Sesselmann, M Tsokos, E Socher, F Garreis, T Müller, F Paulsen Production and Secretion of Gelsolin by Both Human Macrophage- and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various Forms of Arthritis Biomedicines, 2022-03-21;10(3):. 2022-03-21 [PMID: 35327525] (ICC, Human)
• C Bonneau, A Eliès, Y Kieffer, B Bourachot, S Ladoire, F Pelon, D Hequet, JM Guinebreti, C Blanchet, A Vincent-Sa, R Rouzier, F Mechta-Gri A subset of activated fibroblasts is associated with distant relapse in early luminal breast cancer Breast Cancer Res., 2020-07-14;22(1):76. 2020-07-14 [PMID: 32665033] (Western Blot, Human)
• Zhu Q, Wang Z, Zhou L, Ren Y, Gong Y, Qin W, Bai L, Hu J, Wang T The role of cadherin-11 in microcystin-LR-induced migration and invasion in colorectal carcinoma cells. Oncol Lett, 2017-11-21;15(2):1417-1422. 2017-11-21 [PMID: 29399188] (ICC, Human)
• K Pohlodek, YY Tan, CF Singer, D Gschwantle Cadherin-11 expression is upregulated in invasive human breast cancer Oncol Lett, 2016-10-10;12(6):4393-4398. 2016-10-10 [PMID: 28101202] (IHC-P, Human)
• Satcher R, Pan T, Cheng C, Lee Y, Lin S, Yu G, Li X, Hoang A, Tamboli P, Jonasch E, Gallick G, Lin S Cadherin-11 in renal cell carcinoma bone metastasis. PLoS ONE, 2014-02-24;9(2):e89880. 2014-02-24 [PMID: 24587095] (Neutralization, Human)
• Shih W, Yamada S N-cadherin-mediated cell-cell adhesion promotes cell migration in a three-dimensional matrix. J Cell Sci, 2012-03-30;125(0):3661-70. 2012-03-30 [PMID: 22467866] (Western Blot, Canine)
• Nonomura Y, Mizoguchi F, Suzuki A Hypoxia-induced abrogation of contact-dependent inhibition of rheumatoid arthritis synovial fibroblast proliferation. J. Rheumatol., 2009-02-17;36(4):698-705. 2009-02-17 [PMID: 19228663] (Western Blot, Human)

Review for Cadherin-11 Antibody (MAB1790) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 09/28/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: prostate
• Species: Human

Bioinformatics

• Gene Symbol: CDH11
• Uniprot:
  • AAA35622()