Cadherin-11 Antibody (283416)

Images

 
Cadherin‑11 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Cadherin‑11 Monoclonal Antibody (Catalog # MAB1790) at 8 µg/mL overnight at 4 °C. Tissue was stained ...read more
Simple Western lane view shows lysates of PC‑3 human prostate cancer cell line, loaded at 0.2 mg/mL. Specific bands were detected for Cadherin‑11 at approximately 114 & 137 kDa (as indicated) using 20 µg/mL of ...read more
Western blot shows lysates of PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cadherin‑11 Monoclonal Antibody (Catalog # MAB1790) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clone
283416
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Cadherin-11 Antibody (283416) Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cadherin‑11
Phe23-Thr617
Accession # AAA35622
Specificity
Detects human Cadherin-11 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Cadherin‑4, rhCadherin‑8, rhCadherin‑12, rhCadherin‑13, rhCadherin‑17, rhE-Cadherin, rhN-Cadherin, rhP-Cadherin, or rhVE-Cadherin.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CDH11
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 20 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 5
using
MAB1790 in the following applications:

Publications
Read Publications using
MAB1790 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cadherin-11 Antibody (283416)

  • CAD11OSF-4
  • cadherin 11, type 2, OB-cadherin (osteoblast)
  • Cadherin11
  • Cadherin-11
  • CDH11
  • CDHOB
  • OB
  • OB-Cadherin
  • Osteoblast cadherin

Background

The cadherin superfamily comprises a large number of membrane glycoproteins with one or more cadherin repeats, which are involved in Ca2+ dependent cell-cell adhesion. The family can be subdivided into several major subgroups, including the type I and type II classical cadherins, desmosomal cadherins, protocadherins, seven transmembrane (Flamingo) cadherins, FAT-family cadherins, T-cadherin and other unclassified cadherins (1). Cadherin-11, also known as OB-cadherin, is a type II classical cadherin. Classical cadherins are type I transmembrane proteins with an N-terminal extracellular domain containing five tandem cadherin repeats and a C-terminal cytoplasmic domain with a characteristic sequence for binding to catenins. Type I cadherins (E-, N-, P-, R-, M-, and EP-cadherin) differ from type II cadherins (cadherin-5 to -12, -18 to -20 and -22) by the presence of the HAV tripeptide motif in the most N-terminal cadherin repeat (2). Classic cadherins mediate cell-cell adhesion preferentially via homotypic interactions and form adherens juctions that have beta -catenin and p120 (ctn) at the cytoplasmic side of the junction (3, 4). Homotypic cadherin interactions also transduce outside-in and inside-out cell signals. Cadherin signaling induces various cellular processes including cell motility, actin cytoskeleton reorganization, proliferation, and differentiation (3, 4). Cadherin-11 is expressed in a variety or normal tissues of mesodermal origin including areas of the kidney and brain, in normal osteoblasts, and in tumors of the stomach, kidney, colon, breast, and bone (osteosarcoma) (5, 6). It is also differentially expressed in the embryonic brain and may be important in regulating neural development. Human Cadherin-11 exhibits a unique mRNA splice site allowing for two forms of the protein to be expressed, a full-length 796 amino acid (aa) protein and a COOH terminus-truncated variant of 693 aa. The truncated variant has a unique cytoplasmic region due to a frameshift event (3). The full-length human and mouse Cadherin-11 share 97% homology at the aa sequence level.

  1. Angst, B.D. et al. (2001) J. Cell Sci. 113:629.
  2. Gessner, R. and R. Tauber (2000) Ann. N.Y. Acad. Sci. 915:136.
  3. Feltes, C.M. et al. (2002) Cancer Research. 62:6688.
  4. Wheelock, J.J. and K.R. Johnson (2003) Annu. Rev. Cell Dev. Biol. 19:207.
  5. Hoffmann, I. and R. Balling (1995) Dev. Biol. 169:337.
  6. Pishvaian, M.J. et. al. (1999) Cancer Research 59:947.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cadherin-11 Antibody (MAB1790)(5)

We have publications tested in 2 confirmed species: Human, Canine.

We have publications tested in 4 applications: ICC, IHC - Paraffin embedded, Neut, WB.


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ICC
(1)
IHC - Paraffin embedded
(1)
Neut
(1)
WB
(2)
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Human
(4)
Canine
(1)
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Review for Cadherin-11 Antibody (MAB1790) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB1790:
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IHC-P
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Cadherin-11 MAB1790
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reviewed by:
Anonymous
IHC-P Human 09/28/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedprostate
SpeciesHuman

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Recent Reviews

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5
1
4
0
3
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2
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1
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Anonymous
09/28/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CDH11
Entrez
Uniprot