Myeloperoxidase/MPO Antibody (2C7)

Images

 
Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Mouse anti Human Myeloperoxidase.
Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - An intracellular stain was performed on Jurkat cells with Myeloperoxidase antibody (2C7) NB100-64803PCP (blue) and a matched isotype control ...read more
Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Staining of human peripheral blood granulocytes with Mouse anti Human myeloperoxidase following permeabilisation with Leucoperm.
Flow Cytometry: Myeloperoxidase/MPO Antibody (2C7) [NB100-64803] - Analysis using the FITC conjugate of NB100-64803. Staining of permeabilised human peripheral blood granulocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-Fr, IHC-P
Clone
2C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Myeloperoxidase/MPO Antibody (2C7) Summary

Immunogen
Human Myeloperoxidase
Specificity
NB100-64803 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. NB100-64803 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognizes recombinant MPO in Western blots and weakly in ELISA. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MPO
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:500-1:1000
Application Notes
Membrane permeabilisation is required for Flow Cytometry. 2C7 recognises myeloperoxidase under nonreducing conditions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Myeloperoxidase/MPO Antibody (2C7)

  • EC 1.11.1
  • EC 1.11.1.7
  • Lactoperoxidase
  • MPO
  • Myeloperoxidase

Background

Myeloperoxidase is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 - 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2. Traditionally Myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-Myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently it was shown that Myeloperoxidase participates in the initiation and progression of cardiovascular disease. It possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Myeloperoxidase/MPO Antibody (NB100-64803). (Showing 1 - 1 of 1 FAQ).

  1. I am trying to create an MPO-DNA sandwich ELISA for immune complexes and I have been unsuccessful so far. I have been using a mouse polyclonal anti-human MPO antibody to coat, but I think this is the problem. I am interested in your monoclonal MPO Ab and think it might work but I can't afford to buy a full tube and have it not work for my experiment. I was wondering if there is any way you can send a small aliquot to test one of my samples? If it works then I would be interested in purchasing. I hope you understand and can help in some way.
    • Unfortunately, it is company policy that we do not send out free samples, as we have a 100% guarantee on all of our products for the applications and species listed on the datasheet. Yes, we recommend using a monoclonal as the capture antibody in ELISA and NB100-64803 is absolutely recommended for this.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-64803AF405
Alexa Fluor 488 Labeled NB100-64803AF488
Alexa Fluor 647 Labeled NB100-64803AF647
Alexa Fluor 700 Labeled NB100-64803AF700
Allophycocyanin Labeled NB100-64803APC
DyLight 350 Labeled NB100-64803UV
DyLight 405 Labeled NB100-64803V
DyLight 488 Labeled NB100-64803G
DyLight 680 Labeled NB100-64803FR
DyLight 755 Labeled NB100-64803IR
FITC Labeled NB100-64241
PE Labeled NB100-64803PE
PerCP Labeled NB100-64803PCP

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Bioinformatics

Gene Symbol MPO
Entrez