Myeloperoxidase/MPO Antibody (2C7) [Allophycocyanin]

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC
• Clone: 2C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Myeloperoxidase/MPO Antibody (2C7) [Allophycocyanin] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Human myeloperoxidase
• Specificity: NB100-64803 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. NB100-64803 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognizes recombinant MPO in Western blots and weakly in ELISA. The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MPO
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Predicted cross-reactivities: Dog

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for Myeloperoxidase/MPO Antibody (2C7) [Allophycocyanin]

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms. CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O. CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O. COFACTOR: Binds 1 calcium ion per heterodimer. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer. SUBUNIT: Tetramer of two light chains and two heavy chains. SUBCELLULAR LOCATION: Lysosome. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD is an autosomal recessive defect that results in disseminated candidiasis. SIMILARITY: Belongs to the peroxidase family. XPO subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myeloperoxidase/MPO Antibody (NB100-64803APC)(1)

Publication using NB100-64803APC

• Arriaga-Pizano L, Ram�rez-Ram�rez D, Prieto-Ch�vez J et al. [Reporte de la Primera Reuni�n Nacional de Consenso para la Inmunofenotipificaci�n de Leucemias Agudas] Gaceta Médica de México 2019-02-26 [PMID: 30799452]

Bioinformatics

• Gene Symbol: MPO