MCAM/CD146 Antibody (012) - Azide and BSA Free

Western Blot: MCAM/CD146 Antibody (012) [NBP2-89202] - Lane A: CD146 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell LysateLysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:132 kDa Observed band size: kDa

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (012) [NBP2-89202] - Staining of human kidney showing membrane staining of endothelial cells (red arrow) (1:200).

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (012) [NBP2-89202] - Staining of human stomach showing membrane staining of endothelial cells (red arrow), epithelial cells (green arrow). The left panel: 1:200. The right panel: 1:1000.

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody (012) [NBP2-89202] - Staining of human lymphonode showing membrane staining of endothelial cells (red arrow), smooth muscle cells (black arrow) (1:200).

Western Blot: MCAM/CD146 Antibody (012) [NBP2-89202] - NBP2-89202 at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:132 kDa Observed band size:140 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 012
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MCAM/CD146 Antibody (012) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human MCAM/CD146 extracellular domain (Accession#: NP_006491.2; Met 1-Gly 559) .
• Specificity: No cross-reactivity in ELISA with:
  
  Human CD66a
  Human CD166
  Human CD106
  Human BCAM
  Human CD226
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MCAM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for MCAM/CD146 Antibody (012) - Azide and BSA Free

• CD146 antigen
• CD146
• cell surface glycoprotein MUC18
• Cell surface glycoprotein P1H12
• L-Gicerin
• MCAM
• melanoma adhesion molecule
• melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen
• Melanoma-associated antigen A32
• Melanoma-associated antigen MUC18
• MUC18
• MUC18Gicerin

Background

CD146 belongs to the immunoglobulin supergene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety. Expression of the molecule was shown in a relatively limited spectrum of normal human tissues (endothelium, smooth muscle, and subpopulations of activated T cells) and malignant neoplasm (melanoma and breast carcinoma). The lineage specific expression pattern of CD146 can be useful in the differential diagnosis of certain lesions including melanomas and various types of gestational trophoblastic lesions. CD146 expression can promote tumor progression in human melanoma, through enhanced interaction between melanoma cells and endothelial cells. However, in breast carcinoma, CD146 may act as a tumor suppressor. Over expression of CD146 in breast carcinoma cells results in a more cohesive cell growth and in the formation of smaller tumors in nude mice. During implantation and placentation, CD146 is expressed by the intermediate trophoblast in the placental site and binds to its putative receptor in uterine smooth muscle cells thus limiting trophoblastic invasion in the myometrium. Monoclonal antibody specific for CD146 is an important tool for the identification and isolation of cells expressing CD146.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MCAM