CD164 Antibody [Unconjugated]

Western blot shows lysates of U937 human histiocytic lymphoma cell line and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5790) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD164 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

CD164 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD164 extracellular domain
  Asp24-Leu197
  Accession # Q04900
• Specificity: Detects human CD164 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD164 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CD164
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD164 Antibody [Unconjugated]

• CD164 antigen
• CD164 molecule, sialomucin
• CD164
• Endolyn
• MGC-24
• MGC-24v
• MUC-24
• Multi-glycosylated core protein 24
• sialomucin

Background

CD164, also known as endolyn, MGC-24, and MUC-24, is an 80‑100 kDa transmembrane sialomucin protein that is expressed by epithelial cells, T and B cells, monocytes, hematopoietic progenitor cells, and activated basophils. CD164 functions as an adhesion molecule and a negative regulator of cell proliferation. A soluble isoform is generated by alternate splicing. Within the ECD, human CD164 shares approximately 53% sequence identity with mouse and rat CD164.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD164 Antibody (AF5790)(3)

Publications using AF5790

• MJG Bakkers, A Moon-Walke, R Herlo, V Brusic, SH Stubbs, KM Hastie, EO Saphire, TL Kirchhause, SPJ Whelan CD164 is a host factor for lymphocytic choriomeningitis virus entry Proceedings of the National Academy of Sciences of the United States of America, 2022-03-02;119(10):e2119676119. 2022-03-02 [PMID: 35235462] (Flow Cytometry, Human)
• Caterina Mancarella, Giulia Caldoni, Irene Ribolsi, Alessandro Parra, Maria Cristina Manara, Arthur M. Mercurio et al. Insulin-Like Growth Factor 2 mRNA-Binding Protein 3 Modulates Aggressiveness of Ewing Sarcoma by Regulating the CD164-CXCR4 Axis Frontiers in Oncology 7/3/2020 [PMID: 32719743]
• Nyegaard M, Rendtorff N, Nielsen M, Corydon T, Demontis D, Starnawska A, Hedemand A, Buniello A, Niola F, Overgaard M, Leal S, Ahmad W, Wikman F, Petersen K, Cruger D, Oostrik J, Kremer H, Tommerup N, Frodin M, Steel K, Tranebjaerg L, Borglum A A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment. PLoS Genet, 2015-07-21;11(7):e1005386. 2015-07-21 [PMID: 26197441] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CD164
• Uniprot:
  • Q04900()