LSD1 Antibody (1B2E5)

Images

 
Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. Strong KDM1A protein expression was confirmed ...read more
Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. KDM1A protein expresion was ...read more
Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.
Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Analysis of LSD1 expression in Hela (1) and Jurkat (2) whole cell lysates.
Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma tissue, showing nuclear localization using LSD1 antibody with DAB staining.
Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - Immunohistochemical analysis of paraffin-embedded Human Kidney Carcinoma tissue, showing nuclear localization using LSD1 antibody with DAB staining.
Simple Western: LSD1 Antibody (1B2E5) [NB100-1762] - Simple Western lane view shows a specific band for LSD1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, IHC, IHC-P
Clone
1B2E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

LSD1 Antibody (1B2E5) Summary

Immunogen
Purified recombinant fragment of human LSD1 (between amino acids 400-600) expressed in E. coli. [UniProt# O60341]
Localization
Nucleus
Marker
Nucleus Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KDM1A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Simple Western 1:500
  • Chromatin Immunoprecipitation
  • ELISA 1:10000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This LSD1 (1B2E5) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Jurkat Whole Cell Lysate
HeLa Whole Cell Lysate
LSD1 Knockout HeLa Cell Lysate
Publications
Read Publications using
NB100-1762 in the following applications:

  • 2 publications
  • 1 publication
  • IHC
    6 publications
  • 1 publication
  • WB
    6 publications

Reactivity Notes

Human, mouse and monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for LSD1 Antibody (1B2E5)

  • amine oxidase (flavin containing) domain 2
  • AOF2
  • AOF2lysine-specific histone demethylase 1
  • BHC110
  • BHC110FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
  • Flavin-containing amine oxidase domain-containing protein 2
  • KIAA0601KDM1
  • LSD1
  • LSD1BRAF35-HDAC complex protein BHC110
  • lysine (K)-specific demethylase 1
  • Lysine (K)specific Demethylase 1A
  • Lysine (K)-specific Demethylase 1A
  • lysine-specific histone demethylase 1A

Background

The amine oxidase domain 2 AOF2 gene encodes a nuclear protein (LSD1,~95 kDa) containing a Swirm domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. LSD1 is a chromatin-modifying enzyme, which serves as a docking module for the stabilization of the associated corepressor complex(es) on chromatin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LSD1 Antibody (NB100-1762)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ChIP, ELISA, IHC, IHC-P, WB.


Filter By Application
ChIP
(2)
ELISA
(1)
IHC
(6)
IHC-P
(1)
WB
(6)
All Applications
Filter By Species
Human
(10)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB100-1762 Applications Species
Haydn T, Kehr S, Willmann D et al. Next-generation sequencing reveals a novel role of lysine-specific demethylase 1 in adhesion of rhabdomyosarcoma cells Int. J. Cancer Nov 21 2019 [PMID: 31755110] (WB, Human) WB Human
Kim D, Nam H, Lee W et al. PKCa-LSD1-NF-kB-Signaling Cascade Is Crucial for Epigenetic Control of the Inflammatory Response Molecular Cell 2018 Jan 01 [PMID: 29395062] (Mouse) Mouse
Lobo J, Rodrigues A, Antunes L et al. High immunoexpression of Ki67, EZH2, and SMYD3 in diagnostic prostate biopsies independently predicts outcome in patients with prostate cancer Urol. Oncol. 2017 Nov 22 [PMID: 29174711] (Human) Human
Pajtler KW, Weingarten C, Thor T et al. The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun. 2013 May 29 [PMID: 24252778] (WB, IHC-P, Mouse, Human) WB, IHC-P Mouse, Human
Kashyap V, Ahmad S, Nilsson EM et al. The lysine specific demethylase-1 (LSD1/KDM1A) regulates VEGF-A expression in prostate cancer. Mol Oncol 2013 Jan 19 [PMID: 23384557] (IHC, WB, Human) IHC, WB Human
Serce N, Gnatzy A, Steiner S et al. Elevated expression of LSD1 (Lysine-specific demethylase 1) during tumour progression from pre-invasive to invasive ductal carcinoma of the breast BMC Clin Pathol 2012 Aug 24 [PMID: 22920283] (IHC, Human) IHC Human
Janzer A, Lim S, Fronhoffs F, Niazy N, Buettner R, Kirfel J. Lysine-specific demethylase 1 (LSD1) and histone deacetylase 1 (HDAC1) synergistically repress proinflammatory cytokines and classical complement pathway components. Biochem Biophys Res Commun. 2012 Apr 17. [PMID: 22542627] (ChIP, WB, Human) ChIP, WB Human
Bennani-Baiti IM, Machado I, Llombart-Bosch A, Kovar H. Lysine-specific demethylase 1 (LSD1/KDM1A/AOF2/BHC110) is expressed and is an epigenetic drug target in chondrosarcoma, Ewing's sarcoma, osteosarcoma, and rhabdomyosarcoma. Hum Pathol. 2012 Jan 13. [PMID: 22245111] (IHC, Human) IHC Human
Schildhaus HU, Riegel R, Hartmann W, Steiner S, Wardelmann E, Merkelbach-Bruse S, Tanaka S, Sonobe H, Schule R, Buettner R, Kirfel J. Lysine-specific demethylase 1 is highly expressed in solitary fibrous tumors, synovial sarcomas, rhabdomyosarcomas, desmoplastic small round cell tumors, and malignant peripheral nerve sheath tumors. Hum Pathol;42(11):1667-75. 2011 Nov. [PMID: 21531005] (IHC, WB, Human) IHC, WB Human
Kauffman EC, Robinson BD, Downes MJ, Powell LG, Lee MM, Scherr DS, Gudas LJ, Mongan NP. Role of androgen receptor and associated lysine-demethylase coregulators, LSD1 and JMJD2A, in localized and advanced human bladder cancer. Mol Carcinog;50(12):931-44. 2011 Dec. [PMID: 21400613] (IHC, Human) IHC Human
Show All 11 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
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FAQs for LSD1 Antibody (NB100-1762). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in this product: LSD1 Antibody (NB100-1762). Could you tell me which epitope/domain this antibody recognizes? What is the immunogen used to generate this antibody? Is it a peptide? I just want to know the rough segment this antibody binds to. I am developing a sandwich ELISA for LSD1, and would like to have two LSD1 antibodies that recognizes different epitopes.
    • The immunogen is a recombinant fragment of LSD1. Unfortunately the immunogen sequence is proprietary and I cannot disclose details about it. I can however tell you that it's found in the C-terminus of the protein. I hope this is helpful.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KDM1A
Entrez
Uniprot