MBD2 Antibody

Western Blot: MBD2 Antibody [NB100-81657] - Detection of Human and Mouse MBD2 on HeLa whole cell lysate using NB100-81657. MBD2 was also immunoprecipitated by rabbit anti-MBD2 antibody NB100-81656.

Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-81657] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MBD2 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MBD2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 361 to 411 of human methyl-CpG binding domain protein 2 using the numbering given in entry NP_003918.1 (GeneID 8932).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MBD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 29907651).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MBD2 Antibody

• Demethylase
• DKFZp586O0821
• DMTase
• MBD2
• methyl-CpG binding domain protein 2
• methyl-CpG-binding domain protein 2
• Methyl-CpG-binding protein MBD2
• NY-CO-41

Background

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MBD2 Antibody (NB100-81657)(3)

Publications using NB100-81657

• Day RN, Day KH, Pavalko FM Direct visualization by FRET-FLIM of a putative mechanosome complex involving Src, Pyk2 and MBD2 in living MLO-Y4 cells PloS one 2021-12-23 [PMID: 34941939]
• Zhao Z, SentUrk N, Song C, Grummt I lncRNA PAPAS tethered to the rDNA enhancer recruits hypophosphorylated CHD4/NuRD to repress rRNA synthesis at elevated temperatures Genes Dev. 2018-05-31 [PMID: 29907651] (Chemotaxis, Human)
• Kim Jy, Kwak Pb, Weitz Cj. Specificity in Circadian Clock Feedback from Targeted Reconstitution of the nuRD Corepressor. Mol. Cell. 2014-11-20 [PMID: 25453762]

Bioinformatics

• Gene Symbol: MBD2
• Entrez:
  • 8932(Human)
  • 17191(Mouse)
• Uniprot:
  • Q9UBB5(Human)