SETD7/9 Antibody

Images

 
Western Blot: SETD7/9 Antibody [NB100-56664] - Whole cell extract from MCF7 cells. Image from verified customer review.
Immunohistochemistry-Paraffin: SETD7/9 Antibody [NB100-56664] - Analysis of human small instestine using SETD7 antibody at 1:100.
Western Blot: SETD7/9 Antibody [NB100-56664] - Analysis of SETD7 in A) MCF7 and B) HeLa lysate using SETD7 antibody at 1:500.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SETD7/9 Antibody Summary

Immunogen
Two synthethic peptides corresponding to amino acids 131-145 (GEVNEDGEMTGEKIA) and 336-352 (GYDHSPPGKSGPEAPEW) of human SETD7 were used as immunogen for this antibody.
Specificity
This is specific for Set7/9 (a histone H3 lysine 4 methyltransferase) and does not recognize PR-Set7 (a histone H4 lysine 20 methyltransferase).
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SETD7
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
Control
HeLa Whole Cell Lysate
SETD7/9 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-56664 in the following applications:

Publications
Read Publication using
NB100-56664 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Neat whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for SETD7/9 Antibody

  • EC 2.1.1.43
  • H3-K4-HMTase SETD7
  • histone H3-lysine 4-specific methyltransferase
  • histone-lysine N-methyltransferase SETD7
  • KIAA1717SET domain-containing protein 7
  • KMT7FLJ21193
  • Lysine N-methyltransferase 7
  • SET domain containing (lysine methyltransferase) 7
  • SET7/9Histone H3-K4 methyltransferase SETD7
  • SET7Set9
  • SET9

Background

Diverse signal transduction pathways impinging on the N-terminal tails of histones lead to a number of post-translational modifications including acetylation, phosphorylation, poly(ADP-ribosylation), ubiquitination and methylation. These modifications play critical roles in regulating chromatin structure and gene expression. Histone methyltransferases (HMTases) selectively methylate evolutionarily conserved arginine or lysine residues, primarily in the N-terminal tails of histones H3 and H4. Set7/9 is a histone specific HMTase that methylates histone H3 lysine 4. Set7/9 transfers methyl groups to lysine 4 of histone H3 in complex with S-adenosyl-L-methionine. Human Set7/9 is a 336 amino acid protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SETD7/9 Antibody (NB100-56664)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ChIP.


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Review for SETD7/9 Antibody (NB100-56664) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56664:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot SETD7/9 NB100-56664
Enlarge
5
reviewed by:
Anonymous
WB Human 07/17/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell extract form MCF7 cells
SpeciesHuman

Blocking

Blocking DetailsNon-fat dry milk (5%) in PBS

Primary Anitbody

Dilution Ratio1:2000 in PBS at RT for 3 hours

Secondary Antibody

Secondary DescriptionAnti rabbit IgG (HRP-conjugated)
Secondary Manufacturer Cat#ab6721 (Abcam)
Secondary Concentration1:20K

Details

Detection NotesBio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 10 sec

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for SETD7/9 Antibody (NB100-56664) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SETD7/9 Antibody (NB100-56664)

Discover related pathways, diseases and genes to SETD7/9 Antibody (NB100-56664). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for SETD7/9 Antibody (NB100-56664)

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Blogs on SETD7/9

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
07/17/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol SETD7
Entrez
Uniprot