DNMT1 Antibody (60B1220.1)


Western Blot: DNMT1 Antibody (60B1220.1) [NB100-56519] - Analysis of Dnmt1 in 2102EP (human embryonic carcinoma) in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence ...read more
Immunocytochemistry/ Immunofluorescence: DNMT1 Antibody (60B1220.1) [NB100-56519] - Dnmt1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: DNMT1 Antibody (60B1220.1) [NB100-56519] - Tissue section of human hepatocellular carcinoma at 1:50 dilution.
Flow Cytometry: DNMT1 Antibody (60B1220.1) [NB100-56519] - An intracellular stain was performed on HeLa cells with DNMT1 [60B1220.1] Antibody NB100-56519AF488 (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: DNMT1 Antibody (60B1220.1) [NB100-56519] - Analysis of PE conjugate of NB100-56519. An intracellular stain was performed on HeLa cells with DNMT1 antibody (60B1220.1) NB100-56519PE (blue) and a matched ...read more
Simple Western: DNMT1 Antibody (60B1220.1) [NB100-56519] - Simple Western lane view shows a specific band for Dnmt1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Immunoprecipitation: DNMT1 Antibody (60B1220.1) [NB100-56519] - The PCNAS228I mutation differentially affects PCNA binding to PIP box-containing proteins. GST-PIP pull down of His-S-PCNAWT or PCNAS228I. Figure shows ...read more

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, ShSpecies Glossary
Applications WB, Simple Western, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, IHC-FrFl, KD
1.0 mg/ml

Order Details

DNMT1 Antibody (60B1220.1) Summary

This antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370).
IgG1 Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Chromatin Immunoprecipitation 1:20 - 1:1000
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry 1 - 5 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:10
  • Immunohistochemistry Free-Floating 1:500
  • Immunohistochemistry 1:50-1:500
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Simple Western 1:100
  • Western Blot 0.1-0.5 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID 24386225). Use in Flow-intracellular reported in scientific literature (PMID: 24578214). In Simple Western only 10-15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Knockdown validation (PMID: 31799621).
Reviewed Applications
Read 4 Reviews rated 4.5
NB100-56519 in the following applications:

Read Publications using
NB100-56519 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 19924110). Porcine reactivity reported in scientific literature (PMID: 23808878). Sheep reactivity reported in scientific literature (PMID: 20413592). Bovine reactivity reported in scientific literature (PMID:27384909).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for DNMT1 Antibody (60B1220.1)

  • AIM
  • CXXC9
  • CXXC9CXXC finger protein 9
  • CXXC-type zinc finger protein 9
  • DNA (cytosine-5-)-methyltransferase 1
  • DNA (cytosine-5)-methyltransferase 1
  • DNA methyltransferase 1
  • DNA methyltransferase HsaI
  • DNA MTase HsaI
  • DNMT
  • DNMT1
  • EC
  • FLJ16293
  • m.HsaI
  • MCMT
  • MCMTMGC104992


Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. NB100-56519 recognizes Dnmt1, a 1616 amino acid protein according to GenBank no. NP_001370.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
Species: Bv, Ca, Hu, Mu, Pm
Applications: WB
Species: Ch, Dr, Fi, Hu, Mar, Mu, Po, Pm, Rb, Rt, Ye, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
Species: Hu, Mu
Applications: ChIP, IHC, IHC-P, IP, WB
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Ce, Dr, Hu, In, Ma, Mu, Po, Rt, Ye
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
Species: Hu, Mu, Ze
Applications: Flow, WB
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu
Applications: IHC, WB
Species: Hu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
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Publications for DNMT1 Antibody (NB100-56519)(121)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Porcine, Sheep.

We have publications tested in 9 applications: ChIP, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, WB.

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Showing Publications 1 - 10 of 121. Show All 121 Publications.
Publications using NB100-56519 Applications Species
Shen Y, Zhao H, Zhang L Et Al. The roles of DNA methylation and hydroxymethylation at short interspersed nuclear elements in the hypothalamic arcuate nucleus during puberty Molecular Therapy - Nucleic Acids Jul 1 2021 [PMID: 34513307] (WB) WB
Huang Z, Yu J, Cui W, et al. The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins Science Advances Jan 1 2021 [PMID: 33523915] (WB, Mouse) WB Mouse
Zhu A, Hopkins KM, Friedman RA et al. DNMT1 and DNMT3B regulate tumorigenicity of human prostate cancer cells by controlling RAD9 expression through targeted methylation Carcinogenesis Aug 11 2020 [PMID: 32780107]
Magno Guimaraes D, deLucas da Silva Almeida F, Moraes Castilho R DNA methyltransferase expression is associated with cell proliferation in salivary mucoepidermoid carcinoma J. Oral Pathol. Med. Aug 2 2020 [PMID: 32740989]
Ebelt ND, Zuniga E, Johnson BL et al. 5-Azacytidine Potentiates Anti-tumor Immunity in a Model of Pancreatic Ductal Adenocarcinoma Front Immunol Mar 31 2020 [PMID: 32296439] (WB, Mouse) WB Mouse
Yang L, So JY, Skrypek N et al. Induction of DNMT3B by PGE2 and IL6 at distant metastatic sites promotes epigenetic modification and breast cancer colonization Cancer Res. Apr 7 2020 [PMID: 32265226] (WB, Mouse) WB Mouse
Uribe-Etxebarria V, Garcia-Gallastegui P, Perez-Garrastachu M et al. Wnt-3a Induces Epigenetic Remodeling in Human Dental Pulp Stem Cells Cells Mar 7 2020 [PMID: 32156036] (WB, Human) WB Human
Li T, Garcia-Gomez A, Morante-Palacios O SIRT1/2 orchestrate acquisition of DNA methylation and loss of histone H3 activating marks to prevent premature activation of inflammatory genes in macrophages Nucleic Acids Res. Dec 4 2019 [PMID: 31799621] (WB, IP, KD, Human) WB, IP, KD Human
Sun X, Cui Y, Feng H et al. TGF-beta signaling controls Foxp3 methylation and T reg cell differentiation by modulating Uhrf1 activity J. Exp. Med. Sep 12 2019 [PMID: 31515281]
Fisslthaler B, Zippel N, Abdel Malik R et al. Myeloid-Specific Deletion of the AMPK alpha 2 Subunit Alters Monocyte Protein Expression and Atherogenesis Int J Mol Sci Jun 19 2019 [PMID: 31248224] (WB, Mouse) WB Mouse
Show All 121 Publications.

Reviews for DNMT1 Antibody (NB100-56519) (4) 4.54

Average Rating: 4.5
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Human.

Reviews using NB100-56519:
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Images Ratings Applications Species Date Details
Western Blot DNMT1 NB100-56519
reviewed by:
Verified Customer
WB Human 01/30/2019


ApplicationWestern Blot
Sample Testedcell
Chromatin Immunoprecipitation DNMT1 NB100-56519
reviewed by:
Yichi Xu
ChIP Human 01/30/2019


ApplicationChromatin Immunoprecipitation
Sample Testedcell


CommentsMore than 1×10e6 cells and 2.4 μg antibody are optimal.
Western Blot DNMT1 NB100-56519
reviewed by:
Verified Customer
WB Human 04/14/2016


ApplicationWestern Blot
Sample TestedProstate cancer cell lines, LnCAP


Blocking Details5% BSA, 25 Celsius, 1 hr


Detection Noteschemiluminescence, bio rad chem doc, exposure time 30seconds, actin control


CommentsWorked great with no problems.
reviewed by:
Verified Customer
IP Human 12/12/2014


Sample TestedSee PMID 24163373


Blocking DetailsSee PMID 24163373


Detection NotesSee PMID 24163373


CommentsPublished in PMID: 24163373

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for DNMT1 Antibody (NB100-56519). (Showing 1 - 2 of 2 FAQs).

  1. Could you please tell me the size of your DNMT1 protein along with Expression System? I am also doing mutant DNMT1 protein expression using the truncated part (650-1602=952 aa), using bacterial expression system (E.coli, BL21(De3). But right now I am getting some problem that, my size of protein is little higher than my accurate size? I use Auto-induction method to express the protein.
    • As you can see from our testing data, we see bands at around 185-200 kDa. Full details of the samples can be found on the image captions. Dnmt1,UniProt P26358, is also highly phosphorylated and acetylated which can raise the apparent molecular weight to higher than the predicted molecular weight. This is why you are seeing the change in size compared to what you might expect from your truncated protein.
  2. We have recently purchased the above mentioned anti-DNMT1 antibody. However, the product datasheet does not have any exact details on the molecular weight of the antibody and images provided are with very wide range protein marker (near DNMT-1 band 116-200kD).
    • According to Uniprot #P26358 the theoretical molecular weight of human DNMT1 is 183 kDa for isoform 1, ~185 kDa for isoform 2 and 144 kDa for isoform 3. This antibody is designed to detect all 3 of these isoforms.Please not that this protein is subject to multiple posttranslational modifications which may differ by the species, cell type and disease state and these may affect how the protein runs on WB. In case of the WB image (image 1 of 5) we show several human an mouse cell lines, human recombinant protein, and each blot shows at least 1 band which looks like the predicted molecular weight of the target. I agree that the 116-200 range is rather wide and it is impossible to say that the protein runs exactly at 183kDa, but we are confident this is a specific band as, in addition, it is blocked by the blocking peptide.  The entire WB image is displayed to show that there were other protein bands observed in some cell lines, also blocked by the peptide. These may be similar epitopes present at other targets or results of posttranslational processing of the DNMT1 protein in these cell types. Further study would be necessary to determine what exactly are these, but we present this data to make the customers aware that there may be some additional bands on the WB.  If you are running a WB and ar enot confident that what you see is your target, we recommend to include some positive (overexpression lysate or treatment that induces the protein expression) or/and negative (KO, siRNA, cell line or tissue known not to express your target protein) controls.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56519AF350
Alexa Fluor 405 NB100-56519AF405
Alexa Fluor 488 NB100-56519AF488
Alexa Fluor 532 NB100-56519AF532
Alexa Fluor 594 NB100-56519AF594
Alexa Fluor 647 NB100-56519AF647
Alexa Fluor 700 NB100-56519AF700
Alexa Fluor 750 NB100-56519AF750
Allophycocyanin NB100-56519APC
Biotin NB100-56519B
DyLight 350 NB100-56519UV
DyLight 405 NB100-56519V
DyLight 488 NB100-56519G
DyLight 550 NB100-56519R
DyLight 594 NB100-56519DL594
DyLight 650 NB100-56519C
DyLight 680 NB100-56519FR
DyLight 755 NB100-56519IR
FITC NB100-56519F
HRP NB100-56519H
Janelia Fluor 549 NB100-56519JF549
Janelia Fluor 646 NB100-56519JF646
PE NB100-56519PE

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Blogs on DNMT1.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Controlling Epigenetic Signaling with Dnmt1 and Dnmt3b
Dnmt1 belongs to the C5-methyltransferase family that repairs cytosines in dsDNA using a nucleophilic attack mechanism. Dnmt1 is the most abundant mammalian DNA methyltransferase. It is the key methylation maintenance enzyme for both DNA replication/r...  Read full blog post.

PCNA Antibodies: Marking Cell Proliferation & DNA Replication
Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and ...  Read full blog post.

DNMT's: An Overview of 3 DNA Methyltransferases
DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth...  Read full blog post.

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Recent Reviews


Verified Customer
Application: WB
Species: Human

Yichi Xu
Application: ChIP
Species: Human

Verified Customer
Application: WB
Species: Human


Gene Symbol DNMT1