DNMT1 Antibody (60B1220.1)

Images

 
Western Blot: DNMT1 Antibody (60B1220.1) [NB100-56519] - analysis of Dnmt1 in 2102EP (human embryonic carcinoma) in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence ...read more
Immunocytochemistry/ Immunofluorescence: DNMT1 Antibody (60B1220.1) [NB100-56519] - Dnmt1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: DNMT1 Antibody (60B1220.1) [NB100-56519] - IHC-P validation of DNMT1 antibody (clone 60B1220.1) on formalin fixed and paraffin embedded tissue section of human hepatocellular carcinoma at ...read more
Flow Cytometry: DNMT1 Antibody (60B1220.1) [NB100-56519] - Analysis of PE conjugate of NB100-56519. An intracellular stain was performed on HeLa cells with DNMT1 antibody (60B1220.1) NB100-56519PE (blue) and a matched ...read more
Simple Western: DNMT1 Antibody (60B1220.1) [NB100-56519] - Simple Western lane view shows a specific band for Dnmt1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, IHC-FrFl
Clone
60B1220.1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

DNMT1 Antibody (60B1220.1) Summary

Immunogen
This antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
DNMT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1-0.5 ug/ml
  • Simple Western 1:100
  • Chromatin Immunoprecipitation 1:20-1:1000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:10
  • Immunohistochemistry 1:50-1:500
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
  • Immunohistochemistry Free-Floating 1:500
Application Notes
Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot and Immunohistochemistry-Paraffin. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24386225).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-56519 in the following applications:

Publications
Read Publications using
NB100-56519 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 19924110). Porcine reactivity reported in scientific literature (PMID: 23808878)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1 ml PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for DNMT1 Antibody (60B1220.1)

  • AIM
  • CXXC9
  • CXXC9CXXC finger protein 9
  • CXXC-type zinc finger protein 9
  • DNA (cytosine-5-)-methyltransferase 1
  • DNA (cytosine-5)-methyltransferase 1
  • DNA methyltransferase 1
  • DNA methyltransferase HsaI
  • DNA MTase HsaI
  • DNMT
  • DNMT1
  • EC 2.1.1.37
  • FLJ16293
  • m.HsaI
  • MCMT
  • MCMTMGC104992

Background

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. NB100-56519 recognizes Dnmt1, a 1616 amino acid protein according to GenBank no. NP_001370.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-266
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB120-13888
Species: Hu, Mu
Applications: WB, ChIP, Flow, ICC/IF, IHC-P, CyTOF-ready, IHC-WhMt
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-56326
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56340
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-Fr, IHC-P
NBP2-03993
Species: Hu, Bv, Ca, Pm
Applications: WB, IHC, IHC-P
NB500-106
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NB100-81657
Species: Hu, Mu
Applications: WB, IHC-P, IP
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB500-171
Species: Hu, Mu, Rt, Xp, Ye
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NB200-616
Species: Hu, Mu
Applications: WB
NB100-74597
Species: Hu
Applications: WB, IHC-P, IP
NBP2-03982
Species: Hu, Mu, Rt, Bv, Ca, Pm
Applications: WB, IHC, IHC-P
NBP2-46085
Species: Hu
Applications: WB, IHC
NBP1-87425
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-52486
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, ICC, IF
Supplier Logo

Publications for DNMT1 Antibody (NB100-56519)(104)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Porcine, Sheep.

We have publications tested in 8 applications: ChIP, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ChIP
(14)
Flow-IC
(1)
ICC/IF
(7)
IHC
(1)
IHC-Fr
(1)
IHC-P
(5)
IP
(5)
WB
(43)
All Applications
Filter By Species
Human
(36)
Mouse
(21)
Rat
(2)
Bovine
(1)
Porcine
(1)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 104. Show All 104 Publications.
Publications using NB100-56519 Applications Species
Kuang H, Tang Z, Zhang C et al. Taxifolin Activates the Nrf2 Anti-Oxidative Stress Pathway in Mouse Skin Epidermal JB6 P+ Cells through Epigenetic Modifications Int J Mol Sci 2017 Jul 17 [PMID: 28714938] (WB, Human) WB Human
Sheikh MA, Malik YS, Zhu X. RA-Induced Transcriptional Silencing of Checkpoint Kinase-2 through Promoter Methylation by Dnmt3b Is Required for Neuronal Differentiation of P19 Cells J. Mol. Biol. 2017 Jul 13 [PMID: 28712951] (Mouse) Mouse
Miozzo F, Arnould H, de Thonel A et al. Alcohol exposure promotes DNA methyltransferase DNMT3A upregulation through reactive oxygen species-dependent mechanisms Cell Stress Chaperones 2017 Jul 15 [PMID: 28712054] (Mouse) Mouse
He J, Pei L, Jiang H et al. Chemoresistance of colorectal cancer to 5-fluorouracil is associated with silencing of the BNIP3 gene through aberrant methylation. Journal of Cancer. Apr 9 2017 [PMID: 28607593] (WB, Human) WB Human
Szarc Vel Szic K, Declerck K, Crans RAJ et al. Epigenetic silencing of triple negative breast cancer hallmarks by withaferin A. Oncotarget Apr 13 2017 [PMID: 28467815] (WB, Human) WB Human
Anwar SL, Krech T, Hasemeier B et al. hsa-mir-183 is frequently methylated and related to poor survival in human hepatocellular carcinoma. World J. Gastroenterol. Mar 7 2017 [PMID: 28321157] (WB, Human) WB Human
Raciti GA, Spinelli R, Desiderio A et al. Specific CpG hyper-methylation leads to Ankrd26 gene down-regulation in white adipose tissue of a mouse model of diet-induced obesity. Sci Rep. Mar 7 2017 [PMID: 28266632] (ChIP, Mouse) ChIP Mouse
Neri F, Rapelli S, Krepelova A et al. Intragenic DNA methylation prevents spurious transcription initiation. Nature. Feb 22 2017 [PMID: 28225755] (WB, Human) WB Human
Wilson RH, Biasutto AJ, Wang L et al. PCNA dependent cellular activities tolerate dramatic perturbations in PCNA client interactions DNA Repair (Amst.) Feb 1 2017 [PMID: 28073635] (WB, Human) WB Human
Koh HB, Scruggs AM, Huang SK. Transforming Growth Factor-beta1 Increases DNA Methyltransferase 1 and -3a Expression Through Distinct Post-transcriptional Mechanisms in Lung Fibroblasts. J. Biol. Chem. Jul 12 2016 [PMID: 27405758] (IP, WB, ICC/IF, Human) IP, WB, ICC/IF Human
Show All 104 Publications.

Reviews for DNMT1 Antibody (NB100-56519) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 application: WB, IP.

Reviews using NB100-56519:
Filter by Applications
WB
(1)
IP
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot DNMT1 NB100-56519
Enlarge
reviewed by:
Anonymous
WB Human 04/14/2016
View

Summary

ApplicationWestern Blot
Sample TestedProstate cancer cell lines, LnCAP
SpeciesHuman

Blocking

Blocking Details5% BSA, 25 Celsius, 1 hr

Details

Detection Noteschemiluminescence, bio rad chem doc, exposure time 30seconds, actin control

Comments

CommentsWorked great with no problems.
 
reviewed by:
Anonymous
IP Human 12/12/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedSee PMID 24163373
SpeciesHuman

Blocking

Blocking DetailsSee PMID 24163373

Details

Detection NotesSee PMID 24163373

Comments

CommentsPublished in PMID: 24163373

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for DNMT1 Antibody (NB100-56519). (Showing 1 - 1 of 1 FAQs).

  1. Could you please tell me the size of your DNMT1 protein along with Expression System? I am also doing mutant DNMT1 protein expression using the truncated part (650-1602=952 aa), using bacterial expression system (E.coli, BL21(De3). But right now I am getting some problem that, my size of protein is little higher than my accurate size? I use Auto-induction method to express the protein.
    • As you can see from our testing data, we see bands at around 185-200 kDa. Full details of the samples can be found on the image captions. Dnmt1,UniProt P26358, is also highly phosphorylated and acetylated which can raise the apparent molecular weight to higher than the predicted molecular weight. This is why you are seeing the change in size compared to what you might expect from your truncated protein.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56519AF405
Alexa Fluor 488 Labeled NB100-56519AF488
Alexa Fluor 647 Labeled NB100-56519AF647
Alexa Fluor 700 Labeled NB100-56519AF700
Allophycocyanin Labeled NB100-56519APC
Biotin Labeled NB100-56519B
DyLight 350 Labeled NB100-56519UV
DyLight 405 Labeled NB100-56519V
DyLight 488 Labeled NB100-56519G
DyLight 550 Labeled NB100-56519R
DyLight 650 Labeled NB100-56519C
DyLight 680 Labeled NB100-56519FR
DyLight 755 Labeled NB100-56519IR
HRP Labeled NB100-56519H
PE Labeled NB100-56519PE

Additional DNMT1 Products

Bioinformatics Tool for DNMT1 Antibody (NB100-56519)

Discover related pathways, diseases and genes to DNMT1 Antibody (NB100-56519). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DNMT1 Antibody (NB100-56519)

Discover more about diseases related to DNMT1 Antibody (NB100-56519).
 

Pathways for DNMT1 Antibody (NB100-56519)

View related products by pathway.

PTMs for DNMT1 Antibody (NB100-56519)

Learn more about PTMs related to DNMT1 Antibody (NB100-56519).
 

Research Areas for DNMT1 Antibody (NB100-56519)

Find related products by research area.

Blogs on DNMT1.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

Controlling Epigenetic Signaling with Dnmt1 and Dnmt3b
Dnmt1 belongs to the C5-methyltransferase family that repairs cytosines in dsDNA using a nucleophilic attack mechanism. Dnmt1 is the most abundant mammalian DNA methyltransferase. It is the key methylation maintenance enzyme for both DNA replication/r...  Read full blog post.

PCNA Antibodies: Marking Cell Proliferation & DNA Replication
Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and...  Read full blog post.

DNMT's: An Overview of 3 DNA Methyltransferases
DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Anonymous
04/14/2016
Application: WB
Species: Human

 
Anonymous
12/12/2014
Application: IP
Species: Human

Bioinformatics

Gene Symbol DNMT1
Entrez
Uniprot