Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

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Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Detection of Human and Mouse Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A on HeLa whole cell lysate using NB110-40585. Lysine ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1010 and the C-terminus (residue 1064) of human Jumonji Domain Containing 2A using the numbering given in entry NP_055478.1 (GeneID 9682).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM4A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
This antibody is useful for Western Blot and Immunprecipitation. Use in ChIP-seq has been reported in the literature (Ram O et al. Dec. 2011) Chromatin Immunoprecipitation was reported in scientific literature.Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 22373579)
Positive Control
Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Lysate (NBL1-12103)
Reviewed Applications
Read 1 Review rated 5
using
NB110-40585 in the following applications:

Publications
Read Publications using
NB110-40585 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

  • EC 1.14.11
  • EC 1.14.11.-
  • JHDM3A
  • JHDM3AJMJD2
  • JmjC domain-containing histone demethylation protein 3A
  • JMJD2
  • JMJD2A
  • JMJD2Alysine-specific demethylase 4A
  • jumonji domain containing 2
  • jumonji domain containing 2A
  • Jumonji domain-containing protein 2A
  • KDM4A
  • KIAA0677jumonji C domain-containing histone demethylase 3A
  • Lysine (K)specific Demethylase 4A
  • Lysine (K)-specific Demethylase 4A

Background

This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (NB110-40585)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(1)
IP
(1)
WB
(4)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB110-40585 Applications Species
Carbonneau M, M Gagne L, Lalonde ME et al. The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway Nat Commun Sep 14 2016 [PMID: 27624942] (WB, Human) WB Human
Qiu MT, Fan Q, Zhu Z et al. KDM4B and KDM4A promote endometrial cancer progression by regulating androgen receptor, c-myc, and p27kip1. Oncotarget 2015 Oct 13 [PMID: 26397136] (WB) WB
Gibbs-Seymour I, Markiewicz E, Bekker-Jensen S et al. Lamin A/C-dependent interaction with 53BP1 promotes cellular responses to DNA damage Aging Cell. 2015 Jan 23 [PMID: 25645366] (IP, Human) IP Human
Das A, Chai JC, Jung KH et al. JMJD2A attenuation affects cell cycle and tumourigenic inflammatory gene regulation in lipopolysaccharide stimulated neuroectodermal stem cells. Exp Cell Res. 2014 Sep 01 [PMID: 25193078]
Mallette FA, Richard S. JMJD2A Promotes Cellular Transformation by Blocking Cellular Senescence through Transcriptional Repression of the Tumor Suppressor CHD5 Cell Rep 2012 Nov 29 [PMID: 23168260] (WB, Human) WB Human
Luo W, Chang R, Zhong J, Pandey A, Semenza GL. Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression. Proc Natl Acad Sci U S A. 2012 Dec 4 [PMID: 23129632]
Mallette FA, Mattiroli F, Cui G et al. RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers 53BP1 recruitment to DNA damage sites EMBO J. 2012 Apr [PMID: 22373579] (WB, ICC/IF, Human) WB, ICC/IF Human
Kim TD, Shin S, Berry WL et al. The JMJD2A demethylase regulates apoptosis and proliferation in colon cancer cells. J Cell Biochem 2012 Apr [PMID: 22134899]
Tan MK, Lim HJ, Harper JW et al. SCF(FBXO22) regulates histone H3 lysine 9 and 36 methylation levels by targeting histone demethylase KDM4A for ubiquitin-mediated proteasomal degradation. Mol Cell Biol 2011 Sep [PMID: 21768309]
Li Q, Ke Q, Costa M et al. Alterations of histone modifications by cobalt compounds. Carcinogenesis 2009 Jul [PMID: 19376846]
Show All 11 Publications.

Review for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (NB110-40585) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB110-40585:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A NB110-40585
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reviewed by:
Anonymous
WB Human 01/11/2012
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Summary

ApplicationWestern Blot
Sample TestedHeLa cell lysates
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: donkey anti-rabbit-IgG, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: ECL prime

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (NB110-40585) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics Tool for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (NB110-40585)

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Recent Reviews

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1
4
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2
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1
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Anonymous
01/11/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol KDM4A
Entrez
Uniprot