FANCD2 Antibody - BSA Free

Knockdown Validated: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - Effects of FANCD2 knockdown on levels of cell cycle regulators. Caki-1 cells were transfected with control siRNA (siCon) or FANCD2 siRNA (siFD2#2) and treated with AICAR for 24 h. The levels of FANCD2, p15Ink4B, and p21Cip1 were evaluated by immunoblotting. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/2211-5463.12185) licensed under a CC-BY licence.

Knockdown Validated: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - Resistance of FANCA-mutant cells with defective FANCD2 function to hydrogen peroxide (H2O2). Representative images illustrating staining for subnuclear FANCD2 foci in isogenic fibroblast pairs, either deficient for FANCD2 (PD20) or FANCA (PD220) and their respective wild-type (wt) complemented counterparts. Foci were visualized three hours after treatment with H2O2 (25 uM for 2 hours). Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2008/821529/) licensed under a CC-BY licence.

Western Blot: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - AMPK-activating AICAR treatment activates FANCD2, a pivotal molecule of Fanconi anemia DNA damage signaling pathway. AICAR treatment induces FANCD2 monoubiquitination in transformed normal fibroblasts (GM00637I). GM00637I cells were treated with 1 mm 2-deoxyglucose, 0.25 mM AICAR, or 1 mm phenformin for 24 h. Lysates were subjected to western blotting with FANCD2 Antibody, phospho-AMPKalpha1 (T172), and AMPKalpha and beta-actin. In FANCD2 blots, the position of monoubiquitinated FANCD2 (Ub-FANCD2) is indicated by an arrow. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/2211-5463.12185) licensed under a CC-BY licence.

Simple Western: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - Simple Western lane view shows a specific band for FANCD2 in 0.1 mg/ml of HeLa lysate using FANCD2 Antibody. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunocytochemistry/Immunofluorescence: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - Analysis using the Biotin conjugate of FANCD2 Antibody. FANCD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to IR. Proliferating SiHa cells were exposed to 10 Gy of IR and double color immunofluorescence staining was performed after 8 h. Images were captured in a Kodak digital image system on a Leica fluorescence microscope.

Western Blot: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - AICAR-induced FANCD2 monoubiquitination is dependent on AMPK. Inhibitor of AMPK blocks AICAR-induced FANCD2 monoubiquitination in GM00637I normal fibroblasts. Cells were pretreated with 5 um of Compound C (an AMPK inhibitor) 1 h before treatment with 0.25 mm AICAR for 24 h. Cell lysates were subjected to immunoblotting with FANCD2 Antibody to visualize monoubiquitinated FANCD2 (Ub-ANCD2). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/2211-5463.12185) licensed under a CC-BY licence.

Western Blot: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - The FA-BRCA Pathway Is Activated on the Sex Chromosomes during Meiosis. Schematic of the FA-BRCA pathway. FA proteins analyzed in this study are shown in color. Western blot analysis with three independent anti-FANCD2 antibodies (G33, E33, and Novus NB100-182 antibody: NB). K561R, PD20 cells expressing a mutated form of FANCD2 incapable of monoubiquitination; WT, PD20 cells complemented with wild-type FANCD2; Vector, PD20 cells containing empty vector. Image collected and cropped by CiteAb from the following publication (//linkinghub.elsevier.com/retrieve/pii/S2211124716313298), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - HES1K109E mutation does not impair MMC-induced HES1 foci formation. HeLa cells transfected with HA-tagged HES1 or HES1K109E coding vectors were processed for immunofluorescence 16 h following treatment with MMC (120 ng/ml). Cells were double-stained with anti-HA and anti-FANCD2 antibodies (mouse anti-HA at 1:500; rabbit anti-FANCD2, Novus Biologicals NB100-182 at 1:2000) followed with secondary antibodies (Goat anti-mouse Alexafluor-555, ThermoScientific A-32727 and goat anti-rabbit Alexafluor-488, ThermoScientific, A-11008). Cell nuclei were labelled with TO-PRO-3 Iodide stain (1:400, ThermoScientific, T3605). Cells were visualized at 100x magnification. Image collected and cropped by CiteAb from the following publication (//bmcresnotes.biomedcentral.com/articles/10.1186/s13104-018-3243-7), licensed under a CC-BY licence.

Immunohistochemistry: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - The tissue sections were incubated with a primary antibody cocktail of FANCD2 Antibody (NB100-182) at a dilution of 1:1000 & a monoclonal anti-Ki67 mouse antibody at a dilution of 1:150 for 1 h at room temperature. Then the slides were incubated with a secondary antibody cocktail containing FITC conjugated anti-rabbit IgG and Alexafluor 594 donkey anti-mouse secondary. The sections were mounted on glass slides using a DAPI-containing embedding medium (Vysis Dapi 1, Abbott Laboratories, Downers Grove, IL, USA), then analyzed under a fluorescence microscope. FANCD2 (top) foci positive NSCL tumor, and FANCD2 (bottom)foci negative NSCL tumor. Magnification: 1000x. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fonc.2014.00368/abstract), licensed under a CC-BY licence.

Western Blot: FANCD2 Antibody [NB100-182] - Analysis of FANCD2 (Molecular weight: 164.1 KDa) using the HRP conjugate of FANCD2 Antibody (lot C) in HeLa WCE.

Western Blot: FANCD2 Antibody [NB100-182] - Functional assessment of Fanconi anemia pathway. Western blot with FANCD2 Antibody of non-FA control lymphoblasts (LCL), proband LCL-A-2017, proband LCL-B-2017, FANCB mutant (null) LCL, and FANCD2 mutant (null) LCL. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/mgg3.350), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: FANCD2 Antibody [NB100-182] - Mouse Embryonic Fibroblasts Untreated and treated with Mitomycin C for 24hr with FANCD2 Antibody diluted 1:500 in 15%BCS+0.3% Triton X 100 in PBS. This image was submitted via customer Review.

Immunohistochemistry: FANCD2 Antibody [NB100-182] - Staining of human prostate, glandular epithelium using FANCD2 Antibody. Image using the Biotin format of this antibody.

• Reactivity: Hu, Mu, Rt, Av, Ca, Ma, Pm, Ze
• Applications: WB, Simple Western, ChIP, Flow, IB, ICC/IF, IHC, IHC-P, IP, RNAi, KD, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FANCD2 Antibody - BSA Free Summary

• Immunogen: This FANCD2 Antibody was developed against human FANCD2 fusion protein (N-terminal fragment). [Swiss-Prot #Q9BXW9]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FANCD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:200-1:500
  • Immunohistochemistry 2.5-5.0 ug/ml
  • Immunohistochemistry-Paraffin 2.5-5.0 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Knockout Validated
  • RNA Inhibition
  • Simple Western 1:25
  • Western Blot 1:10000-1:20000
• Application Notes: By Western blot, this antibody should recognize a band at ~166 kDa (post-translationally modified form). Additional bands may be seen at lower molecular weights. For immunofluorescence, it has been tested in human MMC and IR treated MEF cells. This antibody may also be used for Immunoprecipitation and Immunohistochemistry-Paraffin. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in RNA inhibition reported in scientific literature (PMID: 27694619). Use in Immunoblotting reported in multiple pieces of scientific literature. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 28196964).
• Theoretical MW: 164.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

This FANCD2 Antibody reacts with human and mouse FANCD2, with Primate and Canine reactivity reported in literature. Zebrafish reactivity reported in scientific literature (PMID: 30540754). Rat reactivity reported in multiple pieces of scientific literature. Kangaroo reactivity reported in scientific literature (PMID: 24982423).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FANCD2 Antibody - BSA Free

• DKFZp762A223
• FA4
• FACD
• FAD
• FAD2
• FA-D2
• FADFAD2
• FANCD
• FANCD2
• Fanconi anemia complementation group D2
• Fanconi anemia group D2 protein
• Fanconi anemia, complementation group D2
• FLJ23826
• FPN1
• HFE4
• IREG1
• Protein FACD2
• SLC11A3

Background

Fanconi Anemia (FA) is an autosomal-recessive cancer-prone disorder characterized by congenital defects, progressive bone marrow failure, increased chromosomal breakage, defective DNA repair and cellular hypersensitivity to mitomycin C. Fanconi Anemia Complementation Group D (FANCD) is comprised of two separate proteins, FANCD1/BRCA2 and FANCD2. LYRIC/MTDH, an RNA binding protein, regulates expression of FANCD2 and FANCD1 (1). FANCD2 is mapped to chromosome 3p25.3, has a theoretical molecular weight of 166 kDa and is localized in the nucleus. FANCD2 is involved in the regulation of DNA-binding transcription factor activity and DNA stability through accurate and efficient pairing of homologs during meiosis to promote repair of double-strand DNA breaks (2). Ubiquitination is required for FANCD2 to bind to chromatin. In response to DNA damage, FANCD2 is monoubiquinated to result in colocalization with other proteins (BRCA1 and BRCA2) involved in homology-directed DNA repair in the nucleus and is deubiquitinated upon DNA repair completion.

References

1. Bi, J., Areecheewakul, S., Li, Y., Yang, S., Zhang, Y., Ebeid, K., . . . Meng, X. (2019). MTDH/AEG-1 downregulation using pristimerin-loaded nanoparticles inhibits Fanconi anemia proteins and increases sensitivity to platinum-based chemotherapy. Gynecol Oncol, 155(2), 349-358. doi:10.1016/j.ygyno.2019.08.014

2. Balcerek, J., Jiang, J., Li, Y., Jiang, Q., Holdreith, N., Singh, B., . . . Tong, W. (2018). Lnk/Sh2b3 deficiency restores hematopoietic stem cell function and genome integrity in Fancd2 deficient Fanconi anemia. Nat Commun, 9(1), 3915. doi:10.1038/s41467-018-06380-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FANCD2 Antibody (NB100-182)(199)

Publications using NB100-182

• Li N, Xu Y, Chen H et al. NEIL3 contributes to the Fanconi anemia/BRCA pathway by promoting the downstream double-strand break repair step Cell reports 2022-11-08 [PMID: 36351389] (ICC/IF, Human)
• Young SJ, Sebald M, Shah Punatar R et al. MutS beta Stimulates Holliday Junction Resolution by the SMX Complex Cell Rep 2020-10-20 [PMID: 33086055] (ICC/IF)
• Gemble S, Amor-Gueret M Identification and Analysis of Different Types of UFBs Methods Mol. Biol. 2020-08-26 [PMID: 32840780]
• Olivieri M, Cho T, Alvarez-QuilOn A et al. A Genetic Map of the Response to DNA Damage in Human Cells Cell 2020-07-23 [PMID: 32649862] (KO, WB, Human)
• Brannvoll A, Xue X, Kwon Y et al. The ZGRF1 Helicase Promotes Recombinational Repair of Replication-Blocking DNA Damage in Human Cells Cell Rep 2020-07-07 [PMID: 32640219] (WB, ICC/IF, Human)
• Kono T, Hoover P, Poropatich K et al. Activation of DNA damage repair factors in HPV positive oropharyngeal cancers Virology 2020-01-01 [PMID: 32560902] (IHC-P, Human)
• Gemble S, Buhagiar-LabarchEde G, Onclercq-Delic R et al. Topoisomerase II alpha prevents ultrafine anaphase bridges by two mechanisms Open Biol 2020-05-01 [PMID: 32400307] (ICC/IF, Human)
• Xing M, Zhang F, Liao H et al. Replication Stress Induces ATR/CHK1-Dependent Nonrandom Segregation of Damaged Chromosomes Mol. Cell 2020-04-22 [PMID: 32353258]
• Kreutmair S, Erlacher M, Andrieux G et al. Loss of the Fanconi anemia-associated protein NIPA causes bone marrow failure J. Clin. Invest. 2020-06-01 [PMID: 32338640] (ICC/IF, IHC-P, Mouse, Human)
• Shao X, Joergensen AM, Howlett NG et al. A distinct role for recombination repair factors in an early cellular response to transcription-replication conflicts Nucleic Acids Res. 2020-04-24 [PMID: 32329774] (ICC/IF, Avian)
• Miller HE, Gorthi A, Bassani N et al. Reconstruction of Ewing Sarcoma Developmental Context from Mass-Scale Transcriptomics Reveals Characteristics of EWSR1-FLI1 Permissibility Cancers (Basel) 2020-04-11 [PMID: 32290418] (WB, Human)
• Cai MY, Dunn CE, Chen W et al. Cooperation of the ATM and Fanconi Anemia/BRCA Pathways in Double-Strand Break End Resection Cell Rep 2020-02-18 [PMID: 32075772] (WB, Human)
• Yang Y, Guo T, Liu R et al. FANCL gene mutations in premature ovarian insufficiency Hum. Mutat. 2020-02-12 [PMID: 32048394] (WB, Human)
• Ardeljan D, Steranka JP, Liu C et al. Cell fitness screens reveal a conflict between LINE-1 retrotransposition and DNA replication Nat. Struct. Mol. Biol. 2020-02-01 [PMID: 32042151] (WB)
• Li N, Wang J, Wallace SS et al. Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair Nucleic Acids Res. 2020-01-25 [PMID: 31980815] (WB, Human)
• Bishop DK Non-enzymatic roles of human RAD51 at stalled replication forks. Mason JM, Chan YL, Weichselbaum RW et al. Nat Commun. [PMID: 31562309] (WB, Human)
• Sonneville R, Bhowmick R, Hoffmann S et al. TRAIP drives replisome disassembly and mitotic DNA repair synthesis at sites of incomplete DNA replication Elife [PMID: 31545170] (ICC/IF)
  
  Details:
  Used at 1:400 for immunofluorescence.
• Chang EY, Tsai S, Aristizabal MJ et al. MRE11-RAD50-NBS1 promotes Fanconi Anemia R-loop suppression at transcription-replication conflicts Nat Commun 2019-09-19 [PMID: 31537797] (WB)
• Bi J, Areecheewakul S, Li Y et al. MTDH/AEG-1 downregulation using pristimerin-loaded nanoparticles inhibits Fanconi anemia proteins and increases sensitivity to platinum-based chemotherapy Gynecol. Oncol. 2019-08-30 [PMID: 31477281] (WB, Human)
• Fragkos M, Barra V, Egger T et al. Dicer prevents genome instability in response to replication stress Oncotarget 2019-07-08 [PMID: 31320994] (WB, Human)
• Tacconi Eliana Mc, Badie Sophie, De Gregoriis Giuliana et al. Chlorambucil targets BRCA1/2-deficient tumours and counteracts PARP inhibitor resistance. Embo Molecular Medicine 2019-07-01 [PMID: 31273933] (WB)
• Pladevall-Morera D, Munk S, Ingham A et al. Proteomic characterization of chromosomal common fragile site (CFS)-associated proteins uncovers ATRX as a regulator of CFS stability Nucleic Acids Res. 2019-06-10 [PMID: 31180492] (IF, Human)
• Tacconi EM, Badie S, De Gregoriis G, Reislander T et al. Chlorambucil targets BRCA1/2-deficient tumours and counteracts PARP inhibitor resistance EMBO Mol Med 2019-05-24 [PMID: 31127019] (WB, Human)
• Paquin KL, Mamrak NE, Garzon JL et al. FANCD2 Binding to H4K20me2 via a Methyl-Binding Domain Is Essential for Efficient DNA Cross-Link Repair Mol. Cell. Biol. 2019-08-01 [PMID: 31085681] (WB, Human)
• Huang J, Zhang J, Bellani MA et al. Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2 Cell Rep 2019-05-07 [PMID: 31067464] (WB, Mouse)
• Andrews AM, McCartneY HJ, Errington TM et al. A senataxin-associated exonuclease SAN1 is required for resistance to DNA interstrand cross-links. Nat Commun. 2018-07-03 [PMID: 29968717] (WB, Human)
• Tremblay CS, Huang FF, Levesque G, Carreau M. Fanconi anemia core complex-dependent HES1 mono-ubiquitination regulates its transcriptional activity. BMC Res Notes. 2018-02-20 [PMID: 29463306] (Human)
• Chang E, Wells J, Tsai S et al. MRE11-RAD50-NBS1 activates Fanconi Anemia R-loop suppression at transcription-replication conflicts. Oncotarget. (WB, Human)
• Fernandes P, Miotto B, Saint-Ruf C et al. FANCD2 tunes the UPR preventing mitochondrial stress-induced common fragile site instability. bioRxiv 2019-10-18 (Chemotaxis, Human)
• Jiang H, Kong N, Liu Z et al. Human Endonuclease ANKLE1 Localizes at the Midbody and Processes Chromatin Bridges to Prevent DNA Damage and cGAS-STING Activation Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023-02-24 [PMID: 36825683] (ICC/IF, Human)
  
  Details:
  Dilution used in ICC/IF 1:1000
• Chung HJ, Lee JR, Kim TM et al. ZNF212 promotes genomic integrity through direct interaction with TRAIP Nucleic acids research 2023-01-25 [PMID: 36594163] (WB, Mouse)
  
  Details:
  See supplemental figure S7
• BOhly N, Schmidt AK, Zhang X et al. Increased replication origin firing links replication stress to whole chromosomal instability in human cancer Cell reports 2022-12-13 [PMID: 36516748] (ICC/IF, Human)
  
  Details:
  Dilution used in ICC 1:500
• Heijink AM, Stok C, Porubsky D et al. Sister chromatid exchanges induced by perturbed replication can form independently of BRCA1, BRCA2 and RAD51 Nature communications 2022-11-07 [PMID: 36344511] (ICC/IF, Human)
  
  Details:
  Dilution used in ICC 1:1000
• Zheng C, Ren Z, Chen H et al. FANCD2 promotes the malignant behavior of endometrial cancer cells and its prognostic value Experimental cell research 2022-10-15 [PMID: 36257352] (KD, IF/IHC, WB, Human)
• Kolinjivadi AM, Sankar H, Choudhary R et al. The H. pylori CagA Oncoprotein Induces DNA Double Strand Breaks through Fanconi Anemia Pathway Downregulation and Replication Fork Collapse International journal of molecular sciences 2022-01-31 [PMID: 35163588] (WB)
• Ruggiano A, Vaz B, Kilgas S et al. The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability Cell reports 2021-12-07 [PMID: 34879279]
• Fierheller CT, Guitton-Sert L, Alenezi WM et al. A functionally impaired missense variant identified in French Canadian families implicates FANCI as a candidate ovarian cancer-predisposing gene Genome medicine 2021-12-03 [PMID: 34861889] (WB, ICC/IF, Human)
• Katsuki Y, Abe M, Park SY Et al. RNF168 E3 ligase participates in ubiquitin signaling and recruitment of SLX4 during DNA crosslink repair Cell reports 2021-10-26 [PMID: 34706224] (ICC/IF, Human)
• Adeyemi RO, Willis NA, Elia AEH Et al. The Protexin complex counters resection on stalled forks to promote homologous recombination and crosslink repair Molecular cell 2021-09-28 [PMID: 34597596]
• GonzAlez-Acosta D, Blanco-Romero E, Ubieto-Capella P et al. PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks The EMBO journal 2021-06-15 [PMID: 34128550] (WB)
• Fang X, Dai E, Bai L et al. The HMGB1-AGER-STING1 pathway mediates the sterile inflammatory response to alkaliptosis Biochemical and biophysical research communications 2021-06-30 [PMID: 33992959]
• Duan W, Tang S, Gao L et al. MiRNA-200C expression in Fanconi anemia pathway functionally deficient lung cancers Scientific reports 2021-02-24 [PMID: 33627769] (IHC-P, WB, Human)
• Jones MJK, Gelot C, Munk S et al. Human DDK rescues stalled forks and counteracts checkpoint inhibition at unfired origins to complete DNA replication Molecular cell 2021-02-04 [PMID: 33545059]
• Ishimoto R, Tsuzuki Y, Matsumura T, et al. SLX4-XPF mediates DNA damage responses to replication stress induced by DNA-protein interactions The Journal of cell biology 2021-01-04 [PMID: 33347546]
• Calzetta NL, Gonzalez Besteiro MA, Gottifredi V Mus81-Eme1-dependent aberrant processing of DNA replication intermediates in mitosis impairs genome integrity Science advances 2020-12-01 [PMID: 33298441] (ICC/IF, Human)
• Ruiz-Torres S, Brusadelli MG, Witte DP et al. Inherited DNA Repair Defects Disrupt the Structure and Function of Human Skin Cell Stem Cell 2020-11-12 [PMID: 33232662] (WB, ICC/IF, Human)
  
  Details:
  Human epidermal skin progenitor cells, skin keratinocytes, oral kertinocytes underwent Western blot analysis. Immunofluorescence analysis was performed on human epidermal skin progenitor cells treated with DOX.
• Lach FP, Singh S, Rickman KA et al. Esophageal Cancer as initial presentation of Fanconi anemia in patients with a hypomorphic FANCA variant Cold Spring Harb Mol Case Stud 2020-11-10 [PMID: 33172906] (WB, Human)
  
  Details:
  Western blot analysis performed on human fibroblasts transfected with lentivirus.
• Watts LP, Natsume T, Saito Y et al. The RIF1-long splice variant promotes G1 phase 53BP1 nuclear bodies to protect against replication stress Elife 2020-11-03 [PMID: 33141022] (ICC/IF, Human)
• Ma C, Hokutan K, Shen Y et al. TFG-maintaining stability of overlooked FANCD2 confers early DNA-damage response Elife 2020-10-19 [PMID: 33099537]
• Rickman KA, Noonan R, Lach FP et al. Distinct roles of BRCA2 in replication fork protection in response to hydroxyurea and DNA interstrand crosslinks Genes Dev 2020-05-02 [PMID: 32354836]
• Achuthankutty D, Thakur RS, Haahr P et al. Regulation of ETAA1-mediated ATR activation couples DNA replication fidelity and genome stability J. Cell Biol. 2019-10-15 [PMID: 31615875]
• Andreev V, Hristova R, Asparuhova M et al. Mammalian INO80 chromatin remodeler cooperates with FANCM to mediate DNA interstrand crosslink-induced checkpoint activation and repair DNA Repair (Amst.) 2019-02-01 [PMID: 30606611] (ICC/IF, Human)
• Ramanagoudr-Bhojappa R, Carrington B, Ramaswami M et al. Multiplexed CRISPR/Cas9-mediated knockout of 19 Fanconi anemia pathway genes in zebrafish revealed their roles in growth, sexual development and fertility PLoS Genet. 2018-12-12 [PMID: 30540754] (WB, Zebrafish)
• Balcerek J, Jiang J, Li Y et al. Lnk/Sh2b3 deficiency restores hematopoietic stem cell function and genome integrity in Fancd2 deficient Fanconi anemia. Nat Commun 2018-09-25 [PMID: 30254368] (WB, Mouse)
• Higgs MR, Sato K, Reynolds JJ et al. Histone Methylation by SETD1A Protects Nascent DNA through the Nucleosome Chaperone Activity of FANCD2 Mol. Cell 2018-06-07 [PMID: 29937342] (Human)
• Kim J, Sturgill D, Sebastian R et al. Replication Stress Shapes a Protective Chromatin Environment across Fragile Genomic Regions Mol. Cell [PMID: 29249653] (ICC/IF, Human)
• Asur RS, Kimble DC, Lach FP et al. Somatic mosaicism of an intragenic FANCB duplication in both fibroblast and peripheral blood cells observed in a Fanconi anemia patient leads to milder phenotype. Mol Genet Genomic Med 2017-11-30 [PMID: 29193904] (ICC/IF, WB, Human)
• Hermanowski H, Huebert B, Aldrighetti C et al. Role of PARylation and PTEN Mutation on PARP and PARG Inhibitor Efficacy on Glioblastoma bioRxiv 2020-07-01 (ICC/IF, Human)
• Hooks KB, Audoux J, Fazli H et al. New insights into diagnosis and therapeutic options for proliferative hepatoblastoma Hepatology. 2017-11-20 [PMID: 29152775] (WB, ICC/IF, Human)
• Vierra DA, Garzon JL, Rego MA et al. Modulation of the Fanconi anemia pathway via chemically induced changes in chromatin structure Oncotarget. 2017-09-29 [PMID: 29100324] (WB, ICC/IF, Human)
• Sakasai R, Isono M, Wakasugi M et al. Aquarius is required for proper CtIP expression and homologous recombination repair. Sci Rep. 2017-10-23 [PMID: 29061988] (WB, Human)
• Porro A, Berti M, Pizzolato J et al. FAN1 interaction with ubiquitylated PCNA alleviates replication stress and preserves genomic integrity independently of BRCA2. Nat Commun. 2017-10-20 [PMID: 29051491] (WB, Human)
• Chang EY, Novoa CA, Aristizabal MJ et al. RECQ-like helicases Sgs1 and BLM regulate R-loop-associated genome instability. J. Cell Biol. 2017-10-17 [PMID: 29042409] (Human)
• Preiss MR, Cournoyer E, Paquin KL et al. Tuning the Multifunctionality of Iron Oxide Nanoparticles Using Self-Assembled Mixed Lipid Layers. Bioconjug. Chem. 2017-10-30 [PMID: 29035511] (WB)
• Moriel-Carretero M, Ovejero S, Gerus-Durand M et al. Fanconi anemia FANCD2 and FANCI proteins regulate the nuclear dynamics of splicing factors. J. Cell Biol. 2017-10-13 [PMID: 29030393] (Human)
• Fierheller CT, Guitton-Sert L, Alenezi WM, Revil T The genetic analysis of a founder Northern American population of European descent identifies FANCI as a candidate familial ovarian cancer risk gene medRxiv 2020-01-01 (WB, Human)
• Bou Samra E, Buhagiar-Labarchede G, Machon C et al. A role for Tau protein in maintaining ribosomal DNA stability and cytidine deaminase-deficient cell survival. Nat Commun 2017-09-25 [PMID: 28947735] (ICC/IF, Human)
• Feng W, Jasin M. BRCA2 suppresses replication stress-induced mitotic and G1 abnormalities through homologous recombination Nat Commun 2017-09-13 [PMID: 28904335] (ICC/IF, Human)
• Chun MJ, Choi H, Jun DW et al. Fanconi anemia protein FANCD2 is activated by AICAR, a modulator of AMPK and cellular energy metabolism. FEBS OpenBio. 2017-01-09 [PMID: 28174693] (WB, ICC/IF, Human)
• Vilfranc CL Elucidating the Role of BRUCE in Chronic Liver Disease Pathogenesis Thesis 2021-01-01 (WB, ICC/IF)
• Wilkes David C, Sailer Verena, Xue Hui et al. A germline FANCA alteration that is associated with increased sensitivity to DNA damaging agents. Cold Spring Harbor Molecular Case Studies 2017-05-03 [PMID: 28864460] (ICC/IF, Human)
• Tacconi EM, Lai X, Folio C et al. BRCA1 and BRCA2 tumor suppressors protect against endogenous acetaldehyde toxicity EMBO Mol Med 2017-07-20 [PMID: 28729482] (WB, Mouse)
• Marchesini M, Ogoti Y, Fiorini E et al. ILF2 Is a Regulator of RNA Splicing and DNA Damage Response in 1q21-Amplified Multiple Myeloma Cancer Cell 2017-07-10 [PMID: 28669490] (Human)
• Niraj J, Caron MC, Drapeau K et al. The identification of FANCD2 DNA binding domains reveals nuclear localization sequences Nucleic Acids Res. 2017-06-28 [PMID: 28666371] (WB, Human)
• Di Marco S, Hasanova Z et al. RECQ5 Helicase Cooperates with MUS81 Endonuclease in Processing Stalled Replication Forks at Common Fragile Sites during Mitosis. Mol Cell 2017-01-06 [PMID: 28575661] (ICC/IF, Human)
• Jiang J, Bellani M, Li L et al. Arsenite Binds to the RING Finger Domain of FANCL E3 Ubiquitin Ligase and Inhibits DNA Interstrand Cross-link Repair. ACS Chem. Biol. 2017-05-23 [PMID: 28535027]
• Ren L, Chen L et al. Potential biomarkers of DNA replication stress in cancer. Oncotarget 2017-06-06 [PMID: 28445142] (IF/IHC, Human)
• Bharathan SP, Nandy K, Palani D et al. Generation of an induced pluripotent stem cell line that mimics the disease phenotypes from a patient with Fanconi anemia by conditional complementation. Stem Cell Research. 2017-02-22 [PMID: 28395741] (ICC/IF, Human)
• Zhang T, Du W et al. Fancd2 in vivo interaction network reveals a non-canonical role in mitochondrial function. Sci Rep 2017-05-04 [PMID: 28378742] (WB, Mouse)
• Spriggs CC, Laimins LA. FANCD2 Binds Human Papillomavirus Genomes and Associates with a Distinct Set of DNA Repair Proteins to Regulate Viral Replication. MBio. 2017-02-14 [PMID: 28196964]
• Lv Z, Rickman KA, Yuan L et al. S. pombe Uba1-Ubc15 Structure Reveals a Novel Regulatory Mechanism of Ubiquitin E2 Activity. Mol. Cell. 2017-02-16 [PMID: 28162934] (WB)
• Olivieri, M, M Charting the genetic architecture of the DNA damage response in human cells Thesis 2022-01-01 (WB, Human)
• Johnson SF, Cruz C, Greifenberg AK et al. CDK12 INHIBITION REVERSES DE NOVO AND ACQUIRED PARP INHIBITOR RESISTANCE IN BRCA WILD-TYPE AND MUTATED MODELS OF TRIPLE-NEGATIVE BREAST CANCER Cell Rep. 2016-11-21 [PMID: 27880910] (WB, Human)
• Vuono EA, Mukherjee A, Vierra DA et al. The PTEN phosphatase functions cooperatively with the Fanconi anemia proteins in DNA crosslink repair. Sci Rep. 2016-11-07 [PMID: 27819275] (Human)
• Bezine E, Malaise Y, Loeuillet A et al. Cell resistance to the Cytolethal Distending Toxin involves an association of DNA repair mechanisms. Sci Rep 2016-10-24 [PMID: 27775089] (WB, Human)
• Alavattam KG, Kato Y, Sin HS et al. Elucidation of the Fanconi Anemia Protein Network in Meoisis and Its Function in the Regulation of Histone Modifications. Cell Rep 2016-10-18 [PMID: 27760317] (WB, Human)
• Sato K, Shimomuki M, Katsuki Y et al. FANCI-FANCD2 stabilizes the RAD51-DNA complex by binding RAD51 and protects the 5'-DNA end. Nucleic Acids Res. 2016-09-30 [PMID: 27694619] (RNAi, WB, Human)
• Fu C, Begum K, Jordan PW et al. Dearth and Delayed Maturation of Testicular Germ Cells in Fanconi Anemia E Mutant Male Mice. PLoS One 2016-08-03 [PMID: 27486799] (IF/IHC)
• Root H, Larsen A, Komosa M et al. FANCD2 limits BLM-dependent telomere instability in the alternative lengthening of telomeres pathway Hum. Mol. Genet. 2016-07-17 [PMID: 27427384]
• Tian C, Hoffman Te, Jacobsen Nk, Spencer Sl Rapidly induced drug adaptation mediates escape from BRAF inhibition in single melanoma cells bioRxiv 2020-01-01
• Chun MJ, Hwang SK, Kim HG et al. Aurora A kinase is required for activation of the Fanconi anemia/BRCA pathway upon DNA damage. FEBS Open Bio. 2016-05-19 [PMID: 27398318] (WB, ICC/IF, Human)
• Michl J, Zimmer J, Buffa FM et al. FANCD2 limits replication stress and genome instability in cells lacking BRCA2. Nat Struct Mol Biol 2016-08-01 [PMID: 27322732] (WB, Human)
• Kais Z, Rondinelli B, Holmes A et al. FANCD2 Maintains Fork Stability in BRCA1/2-Deficient Tumors and Promotes Alternative End-Joining DNA Repair. Cell Rep 2016-06-14 [PMID: 27264184] (WB)
• Maeda J, Froning CE, Brents CA et al. Intrinsic Radiosensitivity and Cellular Characterization of 27 Canine Cancer Cell Lines. PLoS ONE 2016-06-04 [PMID: 27257868] (WB, Canine)
• Wang F, Gao C, Lu J et al. Leukemic survival factor SALL4 contributes to defective DNA damage repair. Oncogene. 2016-05-02 [PMID: 27132514] (WB, Human)
• Panneerselvam J, Wang H, Zhang J et al. BLM promotes the activation of Fanconi Anemia signaling pathway. Oncotarget. 2016-04-12 [PMID: 27083049] (ICC/IF, WB, Human)
• Minocherhomji S, Ying S, Bjerregaard VA et al. Replication stress activates DNA repair synthesis in mitosis. Nature 2015-12-02 [PMID: 26633632] (WB, ICC/IF, Human)
• Bouffard F, Plourde K, Belanger S et al. Analysis of a FANCE Splice Isoform in Regard to DNA Repair. J. Mol. Biol. 2015-08-12 [PMID: 26277624] (WB, Human)
• Stoepker C, Ameziane N, van der Lelij P et al. Defects in the fanconi anemia pathway and chromatid cohesion in head-and-neck cancer Cancer Res. 2015-06-29 [PMID: 26122845] (ICC/IF, WB, Human)
• Rickman KA, Lach FP, Abhyankar A et al. Deficiency of UBE2T, the E2 Ubiquitin Ligase Necessary for FANCD2 and FANCI Ubiquitination, Causes FA-T Subtype of Fanconi Anemia. Cell Rep 2015-07-07 [PMID: 26119737]
• Zhu J, Su F, Mukherjee S et al. FANCD2 influences replication fork processes and genome stability in response to clustered DSBs. Cell Cycle 2015-01-01 [PMID: 26083937] (WB)
• Zhang T, Wilson AF, Ali AM et al. Loss of Faap20 Causes Hematopoietic Stem and Progenitor Cell Depletion in Mice Under Genotoxic Stress Stem Cells 2015-04-27 [PMID: 25917546]
• Oka Y, Bekker-Jensen S, Mailand N. Ubiquitin-like protein UBL5 promotes the functional integrity of the Fanconi anemia pathway EMBO J. 2015-04-09 [PMID: 25862789] (WB, Human)
• Xie J, Kim H, Moreau LA et al. RNF4-mediated polyubiquitination regulates the Fanconi anemia/BRCA pathway. J. Clin. Invest. 2015-04-01 [PMID: 25751062] (ICC/IF, Human)
• Brem R, Zhang X, Xu YZ et al. UVA Photoactivation of DNA Containing Halogenated Thiopyrimidines Induces Cytotoxic DNA lesions Journal of Photochemistry and Photobiology B: Biology. 2015-02-20 [PMID: 25747491] (WB, Human)
  
  Details:
  FANCD2 antibody was used in WB application for the detection of FANCD2 activation in 50ug lysates of HeLa cells which were previously treated with 25 ?M SIdU or SBrdU for 24 hours followed by UVA irradiation (lysates prepared 2 hours after the radiation). The samples showed doublet bands for FANCD2 which represented the FANCD2's un-modified form/lower band and its monoubiquitinated form/top band (Fig 2C).
• Aarts M, Bajrami I, Herrera-Abreu MT et al. Functional genetic screen identifies increased sensitivity to WEE1 inhibition in cells with defects in Fanconi Anaemia and HR pathways Mol. Cancer Ther. 2015-02-11 [PMID: 25673822] (WB, Human)
• Stoepker C, Faramarz A, Rooimans MA, van Mil SE. DNA helicases FANCM and DDX11 are determinants of PARP inhibitor sensitivity. DNA Repair. 2014-12-24 [PMID: 25583207] (ICC/IF, Human)
  
  Details:
  FANCD2 antibody used for ICC-IF in experiments involving head and neck tumor cell lines (VU-SCC-1131 (FANCC-deficient), VU-SCC-1365 (FANCA-deficient) and VU-SCC-1604 (FANCL-deficient)) derived from FA patients (Fig. 5D).
• Duan W, Gao L, Aguila B et al. Fanconi anemia repair pathway dysfunction, a potential therapeutic target in lung cancer. Front Oncol. 2015-01-08 [PMID: 25566506] (WB, IHC-P, Human)
  
  Details:
  The paraffin-embedded human lung tumor tissue sections stained with FANCD2 antibody and analyzed by FATSI method. The FANCD2 antibody was also used for WB analysis on lung cancer cells.
• Bursomanno S, Beli P, Khan AM et al. Proteome-wide analysis of SUMO2 targets in response to pathological DNA replication stress in human cells. DNA Repair (Amst). 2014-11-25 [PMID: 25497329] (WB, Human)
• Zhu B, Yan K, Li L et al. K63-linked ubiquitination of FANCG is required for its association with the Rap80-BRCA1 complex to modulate homologous recombination repair of DNA interstand crosslinks. Oncogene. 2014-08-18 [PMID: 25132264]
• Chlon TM, Hoskins EE, Mayhew CN et al. High risk HPV E6 protein promotes reprogramming of Fanconi Anemia patient cells through repression of p53 but does not allow for sustained growth of iPSC. J. Virol. 2014-07-16 [PMID: 25031356] (WB, Human)
  
  Details:
  FANCD2 antibody used for WB in FA patients-derived cell (FIG 2B) and on induced pluripotent stem cells/iPSC lines as well as parental keratinocytes from patients FA-A2 to -4 treated with 1 mM hydroxyurea (FIG 5J).
• Silva Barbara Alcaraz, Stambaugh Jessica R, Yokomori Kyoko et al. DNA damage to a single chromosome end delays anaphase onset. J Biol Chem. 2014-08-15 [PMID: 24982423]
• Huang Y, Leung JW, Lowery M et al. Modularized functions of the Fanconi anemia core complex. Cell Rep 2014-06-26 [PMID: 24910428]
• Leick L. Analysis of molecular components, causing Fanconi Anemia-like phenotype in HPV-16 infected Head and Neck Squamous Cell Carcinomas. Thesis 2017-01-01 (WB)
• Sarbajna Shriparna, Davies Derek, West Stephen C. Roles of SLX1-SLX4, MUS81-EME1, and GEN1 in avoiding genome instability and mitotic catastrophe. Genes Dev. 2014-05-15 [PMID: 24831703] (ICC/IF, Human)
• Huard CC, Tremblay CS, Magron A, Carreau M. The Fanconi anemia pathway has a dual function in Dickkopf-1 transcriptional repression. Proc. Natl. Acad. Sci. U.S.A. 2014-02-12 [PMID: 24469828] (WB, Human)
• Olivieri, M, M Charting the genetic architecture of the DNA damage response in human cells Thesis 2022-01-01 (WB, Human)
• Panneerselvam J, Pickering A, Han B et al. Basal level of FANCD2 monoubiquitination is required for the maintenance of a sufficient number of licensed-replication origins to fire at a normal rate. Oncotarget 2014-07-15 [PMID: 24658369] (WB, Human)
  
  Details:
  MCM6 antibody used for WB on several experiments involving CRL-1790 and HEK293T cells.
• Boisvert RA, Rego MA, Azzinaro PA et al. Coordinate Nuclear Targeting of the FANCD2 and FANCI Proteins via a FANCD2 Nuclear Localization Signal. PLoS One. 2013-11-21 [PMID: 24278431] (ICC/IF, WB, Human)
• Figueiredo N, Chora A, Raquel H et al. Anthracyclines Induce DNA Damage Response-Mediated Protection against Severe Sepsis. Immunity. 2013-11-14 [PMID: 24184056] (WB, Human)
• Wha Jun D, Hwang M, Kim HJ et al. Ouabain, a Cardiac Glycoside, Inhibits the Fanconi Anemia/BRCA Pathway Activated by DNA Interstrand Cross-Linking Agents. PLoS One. 2013-10-04 [PMID: 24124520] (WB, Human)
• Wang Y, Han X, Wu F et al. Structure analysis of FAAP24 reveals single-stranded DNA-binding activity and domain functions in DNA damage response. Cell Res. 2013-10-01 [PMID: 23999858] (WB, Human)
• Pfaffle HN, Wang M, Gheorghiu L et al. EGFR activating mutations correlate with a Fanconi anemia-like cellular phenotype that includes PARP inhibitor sensitivity. Cancer Res. 2013-08-21 [PMID: 23966292] (ICC/IF, Human)
• Coulthard R, Deans AJ, Swuec P et al. Architecture and DNA Recognition Elements of the Fanconi Anemia FANCM-FAAP24 Complex. Structure. 2013-09-03 [PMID: 23932590] (WB, Human)
• Mason JM, Das I, Arlt M et al. The SNM1B/APOLLO DNA nuclease functions in resolution of replication stress and maintenance of common fragile site stability. Hum Mol Genet 2013-07-30 [PMID: 23863462] (WB, ICC/IF, Human)
• Ying S, Minocherhomji S, Chan KL et al. MUS81 promotes common fragile site expression. Nat Cell Biol 2013-06-30 [PMID: 23811685] (ICC/IF, Human)
• Tomida J, Itaya A, Shigechi T et al. A novel interplay between the Fanconi anemia core complex and ATR-ATRIP kinase during DNA cross-link repair. Nucleic Acids Res 2013-05-30 [PMID: 23723247] (WB, Human)
• Zhang P, Herbig U, Coffman F, Lambert MW. Non-erythroid alpha spectrin prevents telomere dysfunction after DNA interstrand cross-link damage Nucleic Acids Res 2013-04-09 [PMID: 23571757] (ICC/IF, Human)
• Fu D, Dudimah FD, Zhang J et al. Recruitment of DNA polymerase eta by FANCD2 in the early response to DNA damage. Cell Cycle 2013-03-01 [PMID: 23388460] (WB, Human)
• Wang Y, Leung JW, Jiang Y et al. FANCM and FAAP24 Maintain Genome Stability via Cooperative as Well as Unique Functions. Mol Cell 2013-01-16 [PMID: 23333308] (ICC/IF, WB, Human)
• Gravells P, Hoh L, Solovieva S et al. Reduced FANCD2 influences spontaneous SCE and RAD51 foci formation in uveal melanoma and Fanconi anaemia. Oncogene 2013-01-14 [PMID: 23318456] (WB, Human)
• Bhat A, Andersen PL, Qin Z, Xiao W. Rev3, the catalytic subunit of Polzeta, is required for maintaining fragile site stability in human cells Nucleic Acids Res 2013-01-08 [PMID: 23303771] (ICC/IF, Human)
• Hays LE. Multifunctionality of the FA pathway. Blood 2013-01-03 [PMID: 23287621] (IP, Human)
• Yuce O, West SC. Senataxin, defective in the neurodegenerative disorder ataxia with oculomotor apraxia 2, lies at the interface of transcription and the DNA damage response Mol Cell Biol 2013-01-01 [PMID: 23149945] (ICC/IF, Human)
• Duquette ML, Zhu Q, Taylor ER et al. CtIP Is Required to Initiate Replication-Dependent Interstrand Crosslink Repair PLoS Genet 2012-11-01 [PMID: 23144634] (ICC/IF, Human)
• Ogi T, Walker S, Stiff T, Hobson E et al. Identification of the First ATRIP-Deficient Patient and Novel Mutations in ATR Define a Clinical Spectrum for ATR-ATRIP Seckel Syndrome PLoS Genet 2012-11-01 [PMID: 23144622] (WB, Human)
• Kim Y, Spitz GS, Veturi U et al. Regulation of multiple DNA repair pathways by the Fanconi anemia protein SLX4 Blood 2012-10-23 [PMID: 23093618]
• Duan W, Gao L, Zhao W, Leon M et al. Assessment of FANCD2 nuclear foci formation in paraffin-embedded tumors: a potential patient-enrichment strategy for treatment with DNA interstrand crosslinking agents Transl Res 2012-10-11 [PMID: 23063585] (IF/IHC, ICC/IF, Human)
• Rego MA, Kolling FW 4th, Vuono EA et al. Regulation of the Fanconi anemia pathway by a CUE ubiquitin-binding domain in the FANCD2 protein Blood 2012-09-06 [PMID: 22855611]
• Panneerselvam J, Park HK, Zhang J et al. FAVL impairment of the Fanconi anemia pathway promotes the development of human bladder cancer Cell Cycle 2012-08-01 [PMID: 22828653] (WB, ICC/IF, Human)
• Brem R, Karran P. Oxidation-mediated DNA cross-linking contributes to the toxicity of 6-thioguanine in human cells Cancer Res 2012-09-15 [PMID: 22822082] (WB, Human)
• Zhou W, Otto EA, Cluckey A et al. FAN1 mutations cause karyomegalic interstitial nephritis, linking chronic kidney failure to defective DNA damage repair. Nat Genet 2012-08-01 [PMID: 22772369]
• Wang Y, Huang JW, Calses P et al. MiR-96 Downregulates REV1 and RAD51 to Promote Cellular Sensitivity to Cisplatin and PARP Inhibition Cancer Res 2012-08-15 [PMID: 22761336] (ICC/IF, Human)
• Ceccaldi R, Parmar K, Mouly E et al. Bone Marrow Failure in Fanconi Anemia Is Triggered by an Exacerbated p53/p21 DNA Damage Response that Impairs Hematopoietic Stem and Progenitor Cells Cell Stem Cell 2012-06-07 [PMID: 22683204] (ICC/IF, WB, Human)
• Hoskins EE, Morreale RJ, Werner SP et al. The Fanconi Anemia Pathway Limits Human Papillomavirus Replication J Virol 2012-08-01 [PMID: 22623785] (WB, Human)
• An JY, Kim E, Zakrzewska A, Yoo YD, Jang JM, Han DH, Lee MJ, Seo JW, Lee YJ, Kim TY, de Rooij DG, Kim BY, Kwon YT. UBR2 of the N-End Rule Pathway Is Required for Chromosome Stability via Histone Ubiquitylation in Spermatocytes and Somatic Cells. PLoS One;7(5):e37414. 2012-01-01 [PMID: 22616001] (ICC/IF, Mouse)
• Jacquemont C, Simon JA, D'Andrea AD, Taniguchi T. Non-specific chemical inhibition of the Fanconi anemia pathway sensitizes cancer cells to cisplatin Mol Cancer 2012-04-26 [PMID: 22537224] (WB, ICC/IF, Human)
• Leung JW, Wang Y, Fong KW et al. Fanconi anemia (FA) binding protein FAAP20 stabilizes FA complementation group A (FANCA) and participates in interstrand cross-link repair. Proc Natl Acad Sci U S A 2012-03-01 [PMID: 22396592] (WB, ICC/IF, Human)
• Bilardi RA, Kimura KI, Phillips DR, Cutts SM. Processing of anthracycline-DNA adducts via DNA replication and interstrand crosslink repair pathways. Biochem Pharmacol. 2012-02-02 [PMID: 22326903] (ICC/IF, Mouse)
• Jones MJ, Colnaghi L, Huang TT. Dysregulation of DNA polymerase ? recruitment to replication forks results in genomic instability. EMBO J 2011-12-13 [PMID: 22157819] (ICC/IF, Human)
• Hovest MG, Krieg T, Herrmann G. Differential roles for Chk1 and FANCD2 in ATR-mediated signalling for psoralen photoactivation-induced senescence. Exp Dermatol;20(11):883-9. 2011-11-01 [PMID: 21995812] (WB, ICC/IF, Human)
• Le VT, Trilling M, Hengel H. The cytomegaloviral protein pUL138 acts as potentiator of tumor necrosis factor (TNF) receptor 1 surface density to enhance ULb'-encoded modulation of TNF-alpha signaling. J Virol 2011-12-01 [PMID: 21976655] (WB)
• Lu WT, Lemonidis K, Drayton RM, Nouspikel T et al. The Fanconi anemia pathway is downregulated upon macrophage differentiation through two distinct mechanisms. Cell Cycle 2011-10-01 [PMID: 21926477] (Human)
• Jacome A, Fernandez-Capetillo O. Lac operator repeats generate a traceable fragile site in mammalian cells. EMBO Rep. 2011-08-12 [PMID: 21836640] (ICC/IF, Mouse)
• Puspasari N, Rowley SM, Gordon L et al. Long range regulation of human FXN gene expression. PLoS One 2011-01-01 [PMID: 21760943] (ICC/IF)
• Brem R, Daehn I, Karran P. Efficient DNA interstrand crosslinking by 6-thioguanine and UVA radiation. DNA Repair (Amst);10(8):869-76. 2011-08-15 [PMID: 21723207] (WB, Human)
• Rego MA, Harney JA, Mauro M et al. Regulation of the activation of the Fanconi anemia pathway by the p21 cyclin-dependent kinase inhibitor. Oncogene 2012-01-19 [PMID: 21685936] (ICC/IF, WB, Human)
• Kavanaugh GM, Wise-Draper TM, Morreale RJ et al. The human DEK oncogene regulates DNA damage response signaling and repair. Nucleic Acids Res;39(17):7465-76. 2011-09-01 [PMID: 21653549] (ICC/IF, Human)
• Mason JM, Sekiguchi JM. SNM1B/APOLLO functions in the Fanconi anemia pathway in response to DNA interstrand crosslinks. Hum Mol Genet. 2011-04-08 [PMID: 21478198] (WB, ICC/IF, Human)
• Baldeyron C, Soria G, Roche D et al. HP1alpha recruitment to DNA damage by p150CAF-1 promotes homologous recombination repair. J Cell Biol 2011-04-01 [PMID: 21464229] (ICC/IF, WB, Mouse, Human)
• Wright JW, Pejovic T, Jurevic L et al. Ovarian surface epitheliectomy in the non-human primate: continued cyclic ovarian function and limited epithelial replacement. Hum Reprod. 2011-06-01 [PMID: 21421661] (IHC-P, Primate)
• Stoepker C, Hain K, Schuster B et al. SLX4, a coordinator of structure-specific endonucleases, is mutated in a new Fanconi anemia subtype. Nat Genet 2011-02-01 [PMID: 21240277] (ICC/IF, Human)
• Kim Y, Lach FP, Desetty R et al. Mutations of the SLX4 gene in Fanconi anemia. Nat Genet 2011-02-01 [PMID: 21240275] (WB, Human)
• Ceccaldi R, Briot D, Larghero J, et al. Spontaneous abrogation of the G2DNA damage checkpoint has clinical benefits but promotes leukemogenesis in Fanconi anemia patients J Clin Invest 2011-01-01 [PMID: 21183791] (WB, Human)
• Ellwood SR, Liu Z, Syme RA et al. A first genome assembly of the barley fungal pathogen Pyrenophora teres f. teres. Genome Biol 2010-01-01 [PMID: 21067574]
• Colnaghi L, Jones MJ, Cotto-Rios XM et al. Patient-derived C-terminal mutation of FANCI causes protein mislocalization and reveals putative EDGE motif function in DNA repair. Blood 2011-02-17 [PMID: 20971953] (ICC/IF, Human)
• Fairclough SR, Dayel MJ, King N. Multicellular development in a choanoflagellate. Curr Biol 2010-10-26 [PMID: 20971426] (ICC/IF)
• Rivera T, Losada A. Recycling cohesin rings by deacetylation. Mol Cell 2010-09-10 [PMID: 20832715]
• Smogorzewska A, Desetty R, Saito TT et al. A genetic screen identifies FAN1, a Fanconi anemia-associated nuclease necessary for DNA interstrand crosslink repair. Mol Cell 2010-07-09 [PMID: 20603073]
• MacKay C, Declais AC, Lundin C et al. Identification of KIAA1018/FAN1, a DNA repair nuclease recruited to DNA damage by monoubiquitinated FANCD2. Cell 2010-07-09 [PMID: 20603015] (ICC/IF, Human)
• Puri S, Hohle TH, O'Brian MR. Control of bacterial iron homeostasis by manganese. Proc Natl Acad Sci U S A 2010-06-08 [PMID: 20498065] (ICC/IF)
• Lyakhovich A, Surralles J. Constitutive activation of caspase-3 and Poly ADP ribose polymerase cleavage in fanconi anemia cells. Mol Cancer Res;8(1):46-56. 2010-01-01 [PMID: 20068062] (WB, Human)
• Deans AJ, West SC., et al. FANCM Connects the Genome Instability Disorders Bloom's Syndrome and Fanconi Anemia. Mol Cell 2009-12-25 [PMID: 20064461] (WB, Human)
• Bhagwat N, Olsen AL, Wang AT et al. XPF-ERCC1 participates in the Fanconi anemia pathway of crosslink repair. Mol Cell Biol :MCB.00086-00009. 2009-01-01 [PMID: 19805513] (ICC/IF, WB, Human)
• Howlett NG, Harney JA, Rego MA et al. Functional Interaction between the Fanconi Anemia D2 Protein and Proliferating Cell Nuclear Antigen (PCNA) via a Conserved Putative PCNA Interaction Motif. J Biol Chem;284(42):28935-28942. 2009-01-01 [PMID: 19704162] (WB, Human)
• Raya A, Rodriguez-Piza I, Guenechea G et al. Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells. Nature 2009-07-02 [PMID: 19483674] (ICC/IF, Human)
• Tremblay CS, Huang FF, Habi O, Huard CC, Godin C, Levesque G, Carreau M. HES1 is a novel interactor of the Fanconi anemia core complex. Blood. 112(5):2062-70. Epub 2008 Jun 11. Erratum in: Blood. 2009 Oct 29;114(18):3974. 2008-09-01 [PMID: 18550849] (ICC/IF, WB, Human)
• Willers H, Kachnic LA, Luo CM et al. Biomarkers and mechanisms of FANCD2 function. J Biomed Biotechnol 2008-01-01 [PMID: 18483568] (ICC/IF, Human)
• Kolas NK, Chapman JR, Nakada S et al. Orchestration of the DNA-damage response by the RNF8 ubiquitin ligase. Science;318(5856):1637-40. 2007-12-07 [PMID: 18006705]
• Jacquemont, C Taniguchi, T. Proteasome Function Is Required for DNA Damage Response Fanconi Anemia Pathway Activation. Cancer Res; 67: 7395 - 7405. 2007-08-01 [PMID: 17671210] (ICC/IF, Human)
• Collis SJ, Barber LJ, Clark AJ et al. HCLK2 is essential for the mammalian S-phase checkpoint and impacts on Chk1 stability. Nat Cell Biol 2007-04-01 [PMID: 17384638] (WB, Human)
• Oda T, Hayano T, Miyaso H, Takahashi N, Yamashita T. Hsp90 regulates the Fanconi anemia DNA damage response pathway. Blood;109(11):5016-26. 2007-06-01 [PMID: 17327415] (WB, Human)
• Dunn J, Potter M, Rees A, Runger TM. Activation of the Fanconi anemia/BRCA pathway and recombination repair in the cellular response to solar ultraviolet light. Cancer Res;66(23):11140-7. 2006-12-01 [PMID: 17145857]
• Mukhopadhyay SS, Leung KS, Hicks MJ, Hastings PJ, Youssoufian H, Plon SE. Defective mitochondrial peroxiredoxin-3 results in sensitivity to oxidative stress in Fanconi anemia. J Cell Biol;175(2):225-35. 2006-10-23 [PMID: 17060495] (WB, Human)
• Tremblay S, Pintor Dos Reis P, Bradley G, Galloni NN, Perez-Ordonez B, Freeman J, Brown D, Gilbert R, Gullane P, Irish J, Kamel-Reid S. Young patients with oral squamous cell carcinoma: study of the involvement of GSTP1 and deregulation of the Fanconi anemia genes. Arch Otolaryngol Head Neck Surg;132 (9):958-66. 2006-09-01 [PMID: 16982972] (IHC-P, Human)
• Bekker-Jensen, S, Lukas, C, Kitagawa, R, Meler, F, Kastan, M B, Bartek, J, Lukas, J. Spatial organization of the mammalian genome surveillance machinery in response to DNA str breaks, . 173, 195-206. 2006-01-01 [PMID: 16618811] (ICC/IF, Human)
• Stiff T, Reis C, Alderton GK, Woodbine L, O'Driscoll M, Jeggo PA. Nbs1 is required for ATR-dependent phosphorylation events. EMBO J;24(1):199-208. 2005-01-12 [PMID: 15616588] (ICC/IF, Human)
• Demuth I, Digweed M, Concannon P. Human SNM1B is required for normal cellular response to both DNA interstrand crosslink-inducing agents and ionizing radiation. Oncogene 2004-11-11 [PMID: 15467758] (IP, Human)
• Donahue, S et al. A Rad50-dependent pathway of DNA repair is deficient in Fanconi anemia fibroblasts. Nucleic Acids Res;32(10):3248-57. 2004-06-15 [PMID: 15199173] (Human)
• Rothfuss, A et al. Repair kinetics of genomic interstrand DNA cross-links: evidence for DNA double-strand break-dependent activation of the Fanconi anemia/BRCA pathway. Mol Cell Biol;24(1):123-34. 2004-01-01 [PMID: 14673148] (WB, ICC/IF, Human)
• Olopade OI, Wei M. FANCF methylation contributes to chemoselectivity in ovarian cancer. Cancer Cell;3(5):417-20. 2003-05-01 [PMID: 12781358]
• Taniguchi, T et al. Disruption of the Fanconi anemia-BRCA pathway in cisplatin-sensitive ovarian tumors. Nat Med;9(5):568-74. 2003-05-01 [PMID: 12692539] (IP, WB, ICC/IF, Human)
• Lensch, MW et al. Acquired FANCA dysfunction and cytogenetic instability in adult acute myelogenous leukemia. Blood;102(1):7-16. 2003-07-01 [PMID: 12637330] (WB, Human)
• Sridharan, D et al. Nonerythroid alphaII spectrin is required for recruitment of FANCA and XPF to nuclear foci induced by DNA interstrand cross-links. J Cell Sci;116(Pt 5):823-35. 2003-03-01 [PMID: 12571280] (IP, WB, ICC/IF, Human)
• Taniguchi, T et al. S-phase-specific interaction of the Fanconi anemia protein, FANCD2, with BRCA1 and RAD51. Blood;100(7):2414-20. 2002-10-01 [PMID: 12239151] (ICC/IF, WB, Human)
• Digweed M, Rothe S, Demuth I, Scholz R, Schindler D, Stumm M, Grompe M, Jordan A, Sperling K. Attenuation of the formation of DNA-repair foci containing RAD51 in Fanconi anaemia. Carcinogenesis;23(7):1121-6. 2002-07-01 [PMID: 12117768]
• Yang Y, Kuang Y, Montes De Oca R, Hays T, Moreau L, Lu N, Seed B, D'Andrea AD. Targeted disruption of the murine Fanconi anemia gene, Fancg/Xrcc9. Blood;98(12):3435-40. 2001-12-01 [PMID: 11719385] (WB, Mouse)
• McMahon, L et al. Human alpha spectrin II and the FANCA, FANCC, and FANCG proteins bind to DNA containing psoralen interstrand cross-links. Biochemistry;40(24):7025-34. 2001-06-19 [PMID: 11401546] (WB, IP, Human)
• Garcia-Higuera, I et al. Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway. Mol Cell;7(2):249-62. 2001-02-01 [PMID: 11239454] (WB, ICC/IF, Human)

Reviews for FANCD2 Antibody (NB100-182) (8)

Average Rating: 4.5

(Based on 8 reviews)

We have 8 reviews tested in 4 species: Human, Mouse, Other, humam cell lines.

reviewed by: L P IF experiment Mouse 01/21/2020

Summary

• Application: Other
• Sample Tested: Mouse embryonic fibroblasts
• Species: Mouse
• Lot: N/A

reviewed by: Wei Du WB humam cell lines 01/22/2018

Summary

• Application: Western Blot
• Sample Tested: Human Cell lines
• Species: humam cell lines
• Lot: R-5

reviewed by: Verified Customer WB Human 12/15/2017

Summary

• Application: Western Blot
• Sample Tested: 20 ug whole cell lysate
• Species: Human
• Lot: R-5

Comments

• Comments: The WB was performed using 3%-8% Gel.

reviewed by: cynthia morimoto WB Other 12/12/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 24124520
• Species: Other

reviewed by: cynthia morimoto IF Other 12/12/2014

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 24124520
• Species: Other

reviewed by: cynthia morimoto WB Human 12/12/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23318456
• Species: Human

reviewed by: Verified Customer WB Human 08/31/2011

Summary

• Application: Western Blot
• Sample Tested: Human Cell Lysate
• Species: Human

reviewed by: Verified Customer WB Human 02/11/2011

Summary

• Application: Western Blot
• Sample Tested: PD20 cell line (corrected with FancD2)- whole cell extract, Sample Amount: 20 ug / lane
• Species: Human

FAQs for FANCD2 Antibody (NB100-182). (Showing 1 - 3 of 3 FAQs).

1. Which clone is used to produce this antibody? I have some suspicion that it might be the same as the one from Abcam (ab2187). As I want to have two different staining of FANCD2, I would like to be sure that it’s not the same antibody.
   • These are polyclonal antibodies, so there will not be a clone ID associated with them. However, I can tell you that NB100-182 is the same as ab2187.
2. We would like to know if the antibody is specific for isoform a, 1471aa (Q9BXW9) or isoform b, 1451aa (Q9BXW9-2)?
   • This antibody has been generated using human FANCD2 fusion protein (N-terminal fragment) as an immunogen. Because the isoforms a and b of FANCD2 are different in their c-terminal sequence, this antibody should not be able to differentiate the isoforms.
3. Where does the FANCD2 Ab specifically recognizes on the protein? I see that it recognizes the N-terminus but could you tell me which AA range exactly?
   • The amino acid range for this antibody corresponds to amino acids 11-230 [Swiss-Prot #Q9BXW9].https://www.uniprot.org/uniprot/Q9BXW9#sequences

Bioinformatics

• Gene Symbol: FANCD2