FANCD2 Antibody - BSA Free

Images

 
Biological Strategies: Knockdown Validated: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - Effects of FANCD2 knockdown on levels of cell cycle regulators. Caki-1 cells were transfected ...read more
Genetic Strategies: Knockdown Validated: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - Resistance of FANCA-mutant cells with defective FANCD2 function to hydrogen peroxide (H2O2). ...read more
Biological Strategies: Western Blot: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - AMPK-activating AICAR treatment activates FANCD2, a pivotal molecule of Fanconi anemia DNA damage ...read more
Simple Western: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - Simple Western lane view shows a specific band for FANCD2 in 0.1 mg/ml of HeLa lysate using FANCD2 Antibody. This experiment was ...read more
Immunocytochemistry/ Immunofluorescence: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - Analysis using the Biotin conjugate of FANCD2 Antibody. FANCD2 colocalizes in vivo with another protein in ...read more
Biological Strategies: Western Blot: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - AICAR-induced FANCD2 monoubiquitination is dependent on AMPK. Inhibitor of AMPK blocks AICAR-induced ...read more
Western Blot: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - The FA-BRCA Pathway Is Activated on the Sex Chromosomes during Meiosis. Schematic of the FA-BRCA pathway. FA proteins analyzed in this ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - HES1K109E mutation does not impair MMC-induced HES1 foci formation. HeLa ...read more
Immunohistochemistry: FANCD2 Antibody - BSA Free [NB100-182] - FANCD2 Antibody [NB100-182] - The tissue sections were incubated with a primary antibody cocktail of FANCD2 Antibody (NB100-182) at a dilution of 1:1000 & a ...read more
Western Blot: FANCD2 Antibody [NB100-182] - Analysis of FANCD2 (Molecular weight: 164.1 KDa) using the HRP conjugate of FANCD2 Antibody (lot C) in HeLa WCE.
Western Blot: FANCD2 Antibody [NB100-182] - Functional assessment of Fanconi anemia pathway. Western blot with FANCD2 Antibody of non-FA control lymphoblasts (LCL), proband LCL-A-2017, proband LCL-B-2017, FANCB mutant ...read more
Immunocytochemistry/ Immunofluorescence: FANCD2 Antibody [NB100-182] - Mouse Embryonic Fibroblasts Untreated and treated with Mitomycin C for 24hr with FANCD2 Antibody diluted 1:500 in 15%BCS+0.3% Triton X 100 in PBS. ...read more
Immunohistochemistry: FANCD2 Antibody [NB100-182] - Staining of human prostate, glandular epithelium using FANCD2 Antibody. Image using the Biotin format of this antibody.
Representative images of the cellular response to RS (induced by a low dose of hydroxyurea; HU) in U2OS cells, as determined by IF staining for gamma H2AX and FANCD2A selected cell is defined by the yellow arrow, and ...read more
Chemical library screening for small molecules that inhibit the Fanconi anemia pathway. (A) Schematic of the screening for small molecules that inhibit IR-induced FANCD2 foci formation. (B) Representative ...read more
The FA-BRCA Pathway Is Activated on the Sex Chromosomes during Meiosis. (F) Western blot analysis with three independent anti-FANCD2 antibodies (G33, E33, and Novus NB100-182 antibody: NB). K561R, PD20 cells expressing ...read more
HES1 mono-ubiquitination is not required for foci formation. a HES1K109E mutation does not impair MMC-induced HES1 foci formation. HeLa cells transfected with HA-tagged HES1 or HES1K109E coding vectors were processed ...read more
Ddx19 is specifically required for the nuclear localization of MKL1.(a) Western blotting (WB) of cells treated with control (Ctrl) or Ddx19 siRNAs (si). (b) Localization of MKL1-GFP (left panels) and endogenous MKL1 ( ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Av, Ca, Ma, Pm, ZeSpecies Glossary
Applications WB, Simple Western, ChIP, Flow, ICC/IF, IHC, IP, KD, WB, ChIP, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

FANCD2 Antibody - BSA Free Summary

Immunogen
This FANCD2 Antibody was developed against human FANCD2 fusion protein (N-terminal fragment). [Swiss-Prot #Q9BXW9]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FANCD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) reported in scientific literature (PMID 28196964)
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 28196964)
  • Flow Cytometry 2 - 5 ug/ml
  • Immunoblotting reported in multiple pieces of scientific literature
  • Immunocytochemistry/ Immunofluorescence 5 ug/ml
  • Immunohistochemistry 2.5-5.0 ug/ml
  • Immunohistochemistry-Paraffin 2.5-5.0 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Knockout Validated
  • RNA Inhibition reported in scientific literature (PMID 27694619)
  • Simple Western 1:25
  • Western Blot 1 - 2 ug/ml
Application Notes
By Western blot, this antibody should recognize a band at ~166 kDa (post-translationally modified form). Additional bands may be seen at lower molecular weights. For immunofluorescence, it has been tested in human MMC and IR treated MEF cells. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
164.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 8 Reviews rated 4.5
using
NB100-182 in the following applications:

Publications
Read Publications using
NB100-182 in the following applications:

Reactivity Notes

Primate reactivity reported in literature (PMID: 21421661). Canine reactivity reported in literature (PMID: 27257868). Zebrafish reactivity reported in scientific literature (PMID: 30540754). Rat reactivity reported in multiple pieces of scientific literature. Kangaroo reactivity reported in scientific literature (PMID: 24982423).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FANCD2 Antibody - BSA Free

  • DKFZp762A223
  • FA4
  • FACD
  • FAD
  • FAD2
  • FA-D2
  • FADFAD2
  • FANCD
  • FANCD2
  • Fanconi anemia complementation group D2
  • Fanconi anemia group D2 protein
  • Fanconi anemia, complementation group D2
  • FLJ23826
  • FPN1
  • HFE4
  • IREG1
  • Protein FACD2
  • SLC11A3

Background

Fanconi Anemia (FA) is an autosomal-recessive cancer-prone disorder characterized by congenital defects, progressive bone marrow failure, increased chromosomal breakage, defective DNA repair and cellular hypersensitivity to mitomycin C. Fanconi Anemia Complementation Group D (FANCD) is comprised of two separate proteins, FANCD1/BRCA2 and FANCD2. LYRIC/MTDH, an RNA binding protein, regulates expression of FANCD2 and FANCD1 (1). FANCD2 is mapped to chromosome 3p25.3, has a theoretical molecular weight of 166 kDa and is localized in the nucleus. FANCD2 is involved in the regulation of DNA-binding transcription factor activity and DNA stability through accurate and efficient pairing of homologs during meiosis to promote repair of double-strand DNA breaks (2). Ubiquitination is required for FANCD2 to bind to chromatin. In response to DNA damage, FANCD2 is monoubiquinated to result in colocalization with other proteins (BRCA1 and BRCA2) involved in homology-directed DNA repair in the nucleus and is deubiquitinated upon DNA repair completion.

References

1. Bi, J., Areecheewakul, S., Li, Y., Yang, S., Zhang, Y., Ebeid, K., . . . Meng, X. (2019). MTDH/AEG-1 downregulation using pristimerin-loaded nanoparticles inhibits Fanconi anemia proteins and increases sensitivity to platinum-based chemotherapy. Gynecol Oncol, 155(2), 349-358. doi:10.1016/j.ygyno.2019.08.014

2. Balcerek, J., Jiang, J., Li, Y., Jiang, Q., Holdreith, N., Singh, B., . . . Tong, W. (2018). Lnk/Sh2b3 deficiency restores hematopoietic stem cell function and genome integrity in Fancd2 deficient Fanconi anemia. Nat Commun, 9(1), 3915. doi:10.1038/s41467-018-06380-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FANCD2 Antibody (NB100-182)(215)

We have publications tested in 6 confirmed species: Human, Mouse, Avian, Canine, Primate, Zebrafish.

We have publications tested in 12 applications: B/N, Chemotaxis, FLOW, ICC/IF, IF, IF/IHC, IHC-P, IP, KD, KO, RNAi, WB.


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B/N
(1)
Chemotaxis
(1)
FLOW
(1)
ICC/IF
(83)
IF
(1)
IF/IHC
(4)
IHC-P
(6)
IP
(5)
KD
(1)
KO
(1)
RNAi
(1)
WB
(110)
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Filter By Species
Human
(144)
Mouse
(11)
Avian
(1)
Canine
(1)
Primate
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 215. Show All 215 Publications.
Publications using NB100-182 Applications Species
Peake JD, Horne KI, Noguchi C et al. The p53 DNA damage response and Fanconi anemia DNA repair pathway protect against acetaldehyde-induced replication stress in esophageal keratinocytes Cell cycle (Georgetown, Tex.) 2023-09-25 [PMID: 37749911]
Tomita A, Sasanuma H, Owa T et al. Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells Nat Commun 2023-09-15 [PMID: 37714828]
Gelot C, Kovacs MT, Miron S et al. Pol? is phosphorylated by PLK1 to repair double-strand breaks in mitosis Nature 2023-09-06 [PMID: 37674080]
Tsui V, Lyu R, Novakovic S et al. Fancm has dual roles in the limiting of meiotic crossovers and germ cell maintenance in mammals Cell Genomics 2023-06-01 [PMID: 37601968] (ICC/IF) ICC/IF
Stok C, Tsaridou S, van den Tempel N et al. FIRRM/C1orf112 is synthetic lethal with PICH and mediates RAD51 dynamics Cell reports 2023-06-21 [PMID: 37347663] (WB, ICC/IF, Human)

Details:
Dilution: 1:200; HAP1 Cells
WB, ICC/IF Human
Barreto-Galvez A, Niljikar M, Gagliardi J et al. Acetyl transferase EP300 deficiency leads to chronic replication stress mediated by defective fork protection at stalled replication forks bioRxiv : the preprint server for biology 2023-04-29 [PMID: 37163075] (Human)

Details:
Used in Mitotic DNA Synthesis (MiDAS); Dilution: 1:1000
Human
Blaize JL, Noori BM, Hunter KP et al. Differential Regulation of Retinoic Acid Metabolism in Fanconi Anemia bioRxiv : the preprint server for biology 2023-04-27 [PMID: 37066159] (ICC/IF, WB, Human) ICC/IF, WB Human
Moore CE, Yalcindag SE, Czeladko H et al. RFWD3 promotes ZRANB3 recruitment to regulate the remodeling of stalled replication forks The Journal of cell biology 2023-05-01 [PMID: 37036693] (WB) WB
Andrs M, Stoy H, Boleslavska B et al. Excessive reactive oxygen species induce transcription-dependent replication stress Nature communications 2023-03-30 [PMID: 36997515] (WB, Human)

Details:
Dilution used in WB 1:1000
WB Human
Garribba L, De Feudis G, Martis V et al. Short-term molecular consequences of chromosome mis-segregation for genome stability Nature communications 2023-03-11 [PMID: 36906648] (ICC/IF, Human)

Details:
Dilution used in ICC/IF 1:200
ICC/IF Human
Show All 215 Publications.

Reviews for FANCD2 Antibody (NB100-182) (8) 4.58

Average Rating: 4.5
(Based on 8 reviews)
We have 8 reviews tested in 4 species: Human, Mouse, Other, humam cell lines.

Reviews using NB100-182:
Filter by Applications
IF experiment
(1)
WB
(6)
IF
(1)
All Applications
Filter by Species
Human
(4)
Mouse
(1)
Other
(2)
humam cell lines
(1)
All Species
Images Ratings Applications Species Date Details
Other FANCD2 NB100-182
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5
reviewed by:
Verified Customer
IF experiment Mouse 01/21/2020
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Summary

ApplicationOther
Sample TestedMouse embryonic fibroblasts
SpeciesMouse
LotN/A
Western Blot FANCD2 NB100-182
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4
reviewed by:
Verified Customer
WB humam cell lines 01/22/2018
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Summary

ApplicationWestern Blot
Sample TestedHuman Cell lines
Specieshumam cell lines
LotR-5
Western Blot FANCD2 NB100-182
Enlarge
5
reviewed by:
Lisa Moreau
WB Human 12/15/2017
View

Summary

ApplicationWestern Blot
Sample Tested20 ug whole cell lysate
SpeciesHuman
LotR-5

Comments

CommentsThe WB was performed using 3%-8% Gel.
  5
reviewed by:
Verified Customer
WB Other 12/12/2014
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 24124520
SpeciesOther
  4
reviewed by:
Verified Customer
IF Other 12/12/2014
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Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24124520
SpeciesOther
  4
reviewed by:
Verified Customer
WB Human 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23318456
SpeciesHuman
Western Blot FANCD2 NB100-182
Enlarge
4
reviewed by:
Alan Yueh-Luen
WB Human 08/31/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman Cell Lysate
SpeciesHuman
Western Blot FANCD2 NB100-182
Enlarge
5
reviewed by:
Wei-Ting Lu
WB Human 02/11/2011
View

Summary

ApplicationWestern Blot
Sample TestedPD20 cell line (corrected with FancD2)- whole cell extract, Sample Amount: 20 ug / lane
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for FANCD2 Antibody (NB100-182). (Showing 1 - 3 of 3 FAQs).

  1. Which clone is used to produce this antibody? I have some suspicion that it might be the same as the one from Abcam (ab2187). As I want to have two different staining of FANCD2, I would like to be sure that it’s not the same antibody.
    • These are polyclonal antibodies, so there will not be a clone ID associated with them. However, I can tell you that NB100-182 is the same as ab2187.
  2. We would like to know if the antibody is specific for isoform a, 1471aa (Q9BXW9) or isoform b, 1451aa (Q9BXW9-2)?
    • This antibody has been generated using human FANCD2 fusion protein (N-terminal fragment) as an immunogen. Because the isoforms a and b of FANCD2 are different in their c-terminal sequence, this antibody should not be able to differentiate the isoforms.
  3. Where does the FANCD2 Ab specifically recognizes on the protein? I see that it recognizes the N-terminus but could you tell me which AA range exactly?
    • The amino acid range for this antibody corresponds to amino acids 11-230 [Swiss-Prot #Q9BXW9].https://www.uniprot.org/uniprot/Q9BXW9#sequences

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional FANCD2 Products

Research Areas for FANCD2 Antibody (NB100-182)

Find related products by research area.

Blogs on FANCD2.

Sample collection from mammalian culture cells for kinomic analysis
By Jamshed Arslan Pharm.D., PhD.IntroductionKinome describes kinases, and kinomics refers to the kinase signaling. Studying the effects of reagent (exogenously applied growth factor or inhibitor) on kinase activit...  Read full blog post.

FANCD2 and DNA damage repair
Fanconi anemia (FA) is a genetically inherited disorder that yields cytogenetic instability, hypersensitivity to DNA crosslinking compounds and defective DNA repair. A variety of genes have been identified within the FA pathway that are referred t...  Read full blog post.

FANCD2 (Fanconi anemia subunit D2 protein)
Fanconi anemia (FANC) is a rare, autosomal-recessive genetic disorder that is a heterogeneous cancer susceptibility condition that manifests with a wide range of symptoms such as congenital malformations, deteriorating bone marrow failure, DNA-dama...  Read full blog post.

FANCD2: A big component of the DNA repair crew
The genetic disorder known as Fanconi anemia (FANC) is a heterogeneous, autosomal-recessive cancer susceptibility condition characterized by a wide array of symptoms. These include congenital malformations, progressive bone marrow failure, DNA-damage ...  Read full blog post.

FANCD2: DNA Repair and Beyond
Fanconi anemia (FANC) is a heterogenous, autosomal-recessive cancer susceptibility genetic disorder that is characterized by a wide array of symptoms, including congenital defects, progressive bone marrow failure due to DNA-damage hypersensitivity, ch...  Read full blog post.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

Fanconi Antibodies and Cancer Research
We at Novus Biologicals have an extensive antibody databasedevoted to the 13 Fanconi anaemia complementation (FANC) genes, which are involved in the recognition and repair of damaged DNA.The core complex of 8 proteins (FANCA, B, C, E, F, G, L and M)...  Read full blog post.

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Recent Reviews

4.5
5
4
4
4
3
0
2
0
1
0

Verified Customer
01/21/2020
Application: IF experiment
Species: Mouse

Verified Customer
01/22/2018
Application: WB
Species: humam cell lines

Lisa Moreau
12/15/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol FANCD2