FANCD2 Antibody

Images

 
Biological Strategies: Immunocytochemistry/ Immunofluorescence: FANCD2 Antibody [NB100-182] - Analysis using the Biotin conjugate of NB100-182. FancD2 colocalizes in vivo with another protein in SiHa cells after ...read more
Biological Strategies: Western Blot: FANCD2 Antibody [NB100-182] - Analysis of FANCD2 in human PD20 cells. Image courtesy of anonymous customer review
Western Blot: FANCD2 Antibody [NB100-182] - FANCD2 in HeLa WCE using NB 100-182 (lot C).
Western Blot: FANCD2 Antibody [NB100-182] - Human cell lines expressing TOPO1 enzyme were analyzed for FANCD2 expression over time. This image was submitted by customer review.
Immunocytochemistry: FANCD2 Antibody [NB100-182] - Mouse Embryonic Fibroblasts Untreated and treated with Mitomycin C for 24hr with primary antibody diluted 1:500 in 15%BCS+0.3% Triton X 100 in PBS. This image was ...read more
Immunohistochemistry: FANCD2 Antibody [NB100-182] - Analysis using the HRP conjugate of NB100-182. Staining of human prostate, glandular epithelium.
Western Blot: FANCD2 Antibody [NB100-182] - Analysis using the HRP conjugate of NB100-182. FANCD2 in HeLa WCE using NB 100-182 (lot C).
Immunocytochemistry/ Immunofluorescence: FANCD2 Antibody [NB100-182] - Analysis using the Biotin conjugate of NB100-182. FancD2 colocalizes in vivo with another protein in U2OS cells after cell exposure to IR. ...read more
Simple Western: FANCD2 Antibody [NB100-182] - Simple Western lane view shows a specific band for FANCD2 in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Ca, PmSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP, RNAi, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

FANCD2 Antibody Summary

Immunogen
Human FANCD2 fusion protein (N-terminal fragment). [Swiss-Prot #Q9BXW9]
Specificity
This antibody is specific for FANCD2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FANCD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:20000
  • Simple Western 1:25
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:200-1:500
  • Immunohistochemistry 2.5-5.0 ug/ml
  • Immunohistochemistry-Paraffin 2.5-5.0 ug/ml
  • Immunoprecipitation 1:10-1:500
  • RNA Inhibition
  • Immunocytochemistry 1:500
Application Notes
By Western blot, this antibody should recognize a band at ~166 kDa (post-translationally modified form). Additional bands may be seen at lower molecular weights. For immunofluorescence it has been tested in human MMC and IR treated MEF cells. This antibody may also be used for Immunoprecipitation and Immunohistochemistry-Paraffin. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in RNA inhibition reported in scientific literature (PMID: 27694619).
Positive Control
HeLa Lysate (NB800-PC1)
FANCD2 Lysate (NBP2-64710)
FANCD2 Lysate (NBP2-66262)
Reviewed Applications
Read 7 Reviews rated 4.4
using
NB100-182 in the following applications:

Publications
Read Publications using
NB100-182 in the following applications:

  • 56 publications
  • IHC
    2 publications
  • 3 publications
  • IP
    5 publications
  • 1 publication
  • WB
    73 publications

Reactivity Notes

NB 100-182 reacts with human and mouse FANCD2.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FANCD2 Antibody

  • DKFZp762A223
  • FA4
  • FACD
  • FACDFLJ23826
  • FAD
  • FAD2
  • FA-D2
  • FADFAD2
  • FANCD
  • FANCD2
  • Fanconi anemia group D2 protein
  • Fanconi anemia, complementation group D2
  • Protein FACD2

Background

Fanconi anemia (FANC) is a human autosomal-recessive cancer susceptibility disorder characterized by congenital defects, progressive bone marrow failure, and cellular hypersensitivity to mitomycin C (MMC). The FANC subunit D2 protein is vital for cellular resistance to DNA cross-linking and the arrest of DNA synthesis after ionizing radiation (IR). DNA damage activates the monoubiquitination of FANCD2, targeting nuclear foci containing the BRCA1 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FANCD2 Antibody (NB100-182)(133)

We have publications tested in 4 confirmed species: Human, Mouse, Canine, Primate.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-P, IP, RNAi, WB.


Filter By Application
ICC/IF
(56)
IHC
(2)
IHC-P
(3)
IP
(5)
RNAi
(1)
WB
(73)
All Applications
Filter By Species
Human
(98)
Mouse
(6)
Canine
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 133. Show All 133 Publications.
Publications using NB100-182 Applications Species
Tremblay CS, Huang FF, Levesque G, Carreau M. Fanconi anemia core complex-dependent HES1 mono-ubiquitination regulates its transcriptional activity. BMC Res Notes. 2018 Feb 20 [PMID: 29463306] (Human) Human
Asur RS, Kimble DC, Lach FP et al. Somatic mosaicism of an intragenic FANCB duplication in both fibroblast and peripheral blood cells observed in a Fanconi anemia patient leads to milder phenotype. Mol Genet Genomic Med 2017 Nov 30 [PMID: 29193904] (WB, ICC/IF, Human) WB, ICC/IF Human
Hooks KB, Audoux J, Fazli H et al. New insights into diagnosis and therapeutic options for proliferative hepatoblastoma Hepatology. 2017 Nov 20 [PMID: 29152775] (WB, ICC/IF, Human) WB, ICC/IF Human
Vierra DA, Garzon JL, Rego MA et al. Modulation of the Fanconi anemia pathway via chemically induced changes in chromatin structure Oncotarget. 2017 Sep 29 [PMID: 29100324] (WB, ICC/IF, Human) WB, ICC/IF Human
Sakasai R, Isono M, Wakasugi M et al. Aquarius is required for proper CtIP expression and homologous recombination repair. Sci Rep. 2017 Oct 23 [PMID: 29061988] (WB, Human) WB Human
Leick L. Analysis of molecular components, causing Fanconi Anemia-like phenotype in HPV-16 infected Head and Neck Squamous Cell Carcinomas. Thesis 2017 (WB) WB
Porro A, Berti M, Pizzolato J et al. FAN1 interaction with ubiquitylated PCNA alleviates replication stress and preserves genomic integrity independently of BRCA2. Nat Commun. 2017 Oct 20 [PMID: 29051491] (WB, Human) WB Human
Chang EY, Novoa CA, Aristizabal MJ et al. RECQ-like helicases Sgs1 and BLM regulate R-loop-associated genome instability. J. Cell Biol. 2017 Oct 17 [PMID: 29042409] (Human) Human
Preiss MR, Cournoyer E, Paquin KL et al. Tuning the Multifunctionality of Iron Oxide Nanoparticles Using Self-Assembled Mixed Lipid Layers. Bioconjug. Chem. 2017 Oct 30 [PMID: 29035511] (WB) WB
Moriel-Carretero M, Ovejero S, Gerus-Durand M et al. Fanconi anemia FANCD2 and FANCI proteins regulate the nuclear dynamics of splicing factors. J. Cell Biol. 2017 Oct 13 [PMID: 29030393] (Human) Human
Show All 133 Publications.

Reviews for FANCD2 Antibody (NB100-182) (7) 4.47

Average Rating: 4.4
(Based on 7 reviews)
We have 7 reviews tested in 3 species: Human, Other, humam cell lines.

Reviews using NB100-182:
Filter by Applications
WB
(6)
IF
(1)
All Applications
Filter by Species
Human
(4)
Other
(2)
humam cell lines
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot FANCD2 NB100-182
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reviewed by:
Anonymous
WB humam cell lines 01/22/2018
View

Summary

ApplicationWestern Blot
Sample TestedHuman Cell lines
Specieshumam cell lines
LotR-5
Western Blot FANCD2 NB100-182
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reviewed by:
Lisa Moreau
WB Human 12/15/2017
View

Summary

ApplicationWestern Blot
Sample Tested20 ug whole cell lysate
SpeciesHuman
LotR-5

Comments

CommentsThe WB was performed using 3%-8% Gel.
 
reviewed by:
Anonymous
WB Other 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24124520
SpeciesOther

Blocking

Blocking DetailsSee PMID 24124520

Details

Detection NotesSee PMID 24124520

Comments

CommentsPublished in PMID: 24124520
 
reviewed by:
Anonymous
IF Other 12/12/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24124520
SpeciesOther

Blocking

Blocking DetailsSee PMID 24124520

Details

Detection NotesSee PMID 24124520

Comments

CommentsPublished in PMID: 24124520
 
reviewed by:
Anonymous
WB Human 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23318456
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23318456

Details

Detection NotesSee PMID 23318456

Comments

CommentsPublished in PMID: 23318456
Western Blot FANCD2 NB100-182
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reviewed by:
Alan Yueh-Luen
WB Human 08/31/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman Cell Lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-Rabbit IgG HRP Conjugated
Western Blot FANCD2 NB100-182
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reviewed by:
Wei-Ting Lu
WB Human 02/11/2011
View

Summary

ApplicationWestern Blot
Sample TestedPD20 cell line (corrected with FancD2)- whole cell extract, Sample Amount: 20 ug / lane
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 3% BSA in TBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/10000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FANCD2 Antibody (NB100-182). (Showing 1 - 2 of 2 FAQs).

  1. Which clone is used to produce this antibody? I have some suspicion that it might be the same as the one from Abcam (ab2187). As I want to have two different staining of FANCD2, I would like to be sure that it’s not the same antibody.
    • These are polyclonal antibodies, so there will not be a clone ID associated with them. However, I can tell you that NB100-182 is the same as ab2187.
  2. We would like to know if the antibody is specific for isoform a, 1471aa (Q9BXW9) or isoform b, 1451aa (Q9BXW9-2)?
    • This antibody has been generated using human FANCD2 fusion protein (N-terminal fragment) as an immunogen. Because the isoforms a and b of FANCD2 are different in their c-terminal sequence, this antibody should not be able to differentiate the isoforms.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-182AF405
Alexa Fluor 488 Labeled NB100-182AF488
Alexa Fluor 647 Labeled NB100-182AF647
Alexa Fluor 700 Labeled NB100-182AF700
Biotin Labeled NB100-182B
DyLight 405 Labeled NB100-182V
DyLight 488 Labeled NB100-182G
DyLight 550 Labeled NB100-182R
DyLight 650 Labeled NB100-182C
FITC Labeled NB100-182F
HRP Labeled NB100-182H

Additional Array Products

Bioinformatics Tool for FANCD2 Antibody (NB100-182)

Discover related pathways, diseases and genes to FANCD2 Antibody (NB100-182). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for FANCD2 Antibody (NB100-182)

Discover more about diseases related to FANCD2 Antibody (NB100-182).
 

Pathways for FANCD2 Antibody (NB100-182)

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PTMs for FANCD2 Antibody (NB100-182)

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Research Areas for FANCD2 Antibody (NB100-182)

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Blogs on FANCD2.

FANCD2 and DNA damage repair
Fanconi anemia (FA) is a genetically inherited disorder that yields cytogenetic instability, hypersensitivity to DNA crosslinking compounds and defective DNA repair. A variety of genes have been identified within the FA pathway that are referred t...  Read full blog post.

FANCD2 (Fanconi anemia subunit D2 protein)
Fanconi anemia (FANC) is a rare, autosomal-recessive genetic disorder that is a heterogeneous cancer susceptibility condition that manifests with a wide range of symptoms such as congenital malformations, deteriorating bone marrow failure, DNA-dama...  Read full blog post.

FANCD2: A big component of the DNA repair crew
The genetic disorder known as Fanconi anemia (FANC) is a heterogeneous, autosomal-recessive cancer susceptibility condition characterized by a wide array of symptoms. These include congenital malformations, progressive bone marrow failure, DNA-damage...  Read full blog post.

FANCD2: DNA Repair and Beyond
Fanconi anemia (FANC) is a heterogenous, autosomal-recessive cancer susceptibility genetic disorder that is characterized by a wide array of symptoms, including congenital defects, progressive bone marrow failure due to DNA-damage hypersensitivity, ch...  Read full blog post.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

Fanconi Antibodies and Cancer Research
We at Novus Biologicals have an extensive antibody databasedevoted to the 13 Fanconi anaemia complementation (FANC) genes, which are involved in the recognition and repair of damaged DNA.The core complex of 8 proteins (FANCA, B, C, E, F, G, L and M)...  Read full blog post.

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Recent Reviews

4.4
5
3
4
4
3
0
2
0
1
0

Anonymous
01/22/2018
Application: WB
Species: humam cell lines

Lisa Moreau
12/15/2017
Application: WB
Species: Human

 
Anonymous
12/12/2014
Application: WB
Species: Other

Bioinformatics

Gene Symbol FANCD2
Entrez