FANCD2 Antibody

Images

 
Biological Strategies: Knockdown Validated: FANCD2 Antibody [NB100-182] - Effects of FANCD2 knockdown on levels of cell cycle regulators. Caki-1 cells were transfected with control siRNA (siCon) or FANCD2 siRNA ...read more
Genetic Strategies: Knockdown Validated: FANCD2 Antibody [NB100-182] - Resistance of FANCA-mutant cells with defective FANCD2 function to hydrogen peroxide (H2O2). Representative images illustrating staining for ...read more
Biological Strategies: Western Blot: FANCD2 Antibody [NB100-182] - AMPK-activating AICAR treatment activates FANCD2, a pivotal molecule of Fanconi anemia DNA damage signaling pathway. AICAR treatment induces ...read more
Simple Western: FANCD2 Antibody [NB100-182] - Simple Western lane view shows a specific band for FANCD2 in 0.1 mg/ml of HeLa lysate using FANCD2 Antibody. This experiment was performed under reducing conditions using ...read more
Immunocytochemistry/ Immunofluorescence: FANCD2 Antibody [NB100-182] - Analysis using the Biotin conjugate of FANCD2 Antibody. FANCD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to IR. ...read more
Biological Strategies: Western Blot: FANCD2 Antibody [NB100-182] - AICAR-induced FANCD2 monoubiquitination is dependent on AMPK. Inhibitor of AMPK blocks AICAR-induced FANCD2 monoubiquitination in GM00637I normal ...read more
Western Blot: FANCD2 Antibody [NB100-182] - The FA-BRCA Pathway Is Activated on the Sex Chromosomes during Meiosis. Schematic of the FA-BRCA pathway. FA proteins analyzed in this study are shown in color. Western blot ...read more
Immunocytochemistry/ Immunofluorescence: FANCD2 Antibody [NB100-182] - HES1 mono-ubiquitination is not required for foci formation. HES1K109E mutation does not impair MMC-induced HES1 foci formation. HeLa cells ...read more
Immunohistochemistry: FANCD2 Antibody [NB100-182] - Detection of FANCD2 foci formation in human lung tumors by the FATSI staining analysis. The tissue sections were incubated with a primary antibody cocktail of FANCD2 ...read more
Western Blot: FANCD2 Antibody [NB100-182] - Analysis of FANCD2 (Molecular weight: 164.1 KDa) using the HRP conjugate of FANCD2 Antibody (lot C) in HeLa WCE.
Western Blot: FANCD2 Antibody [NB100-182] - Functional assessment of Fanconi anemia pathway. Western blot with FANCD2 Antibody of non-FA control lymphoblasts (LCL), proband LCL-A-2017, proband LCL-B-2017, FANCB mutant ...read more
Immunocytochemistry/ Immunofluorescence: FANCD2 Antibody [NB100-182] - Mouse Embryonic Fibroblasts Untreated and treated with Mitomycin C for 24hr with FANCD2 Antibody diluted 1:500 in 15%BCS+0.3% Triton X 100 in PBS. ...read more
Immunohistochemistry: FANCD2 Antibody [NB100-182] - Staining of human prostate, glandular epithelium using FANCD2 Antibody. Image using the Biotin format of this antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Av, Ca, Ma, Pm, ZeSpecies Glossary
Applications WB, Simple Western, ChIP, Flow, IB, ICC/IF, IHC, IHC-P, IP, RNAi, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

FANCD2 Antibody Summary

Immunogen
This FANCD2 Antibody was developed against human FANCD2 fusion protein (N-terminal fragment). [Swiss-Prot #Q9BXW9]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FANCD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:20000
  • Simple Western 1:25
  • Chromatin Immunoprecipitation
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:200-1:500
  • Immunohistochemistry 2.5-5.0 ug/ml
  • Immunohistochemistry-Paraffin 2.5-5.0 ug/ml
  • Immunoprecipitation 1:10-1:500
  • RNA Inhibition
  • Knockdown Validated
  • Knockout Validated
Application Notes
By Western blot, this antibody should recognize a band at ~166 kDa (post-translationally modified form). Additional bands may be seen at lower molecular weights. For immunofluorescence, it has been tested in human MMC and IR treated MEF cells. This antibody may also be used for Immunoprecipitation and Immunohistochemistry-Paraffin. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in RNA inhibition reported in scientific literature (PMID: 27694619). Use in Immunoblotting reported in multiple pieces of scientific literature. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 28196964).
Theoretical MW
164.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
FANCD2 Knockout HeLa Cell Lysate
FANCD2 Knockout HeLa Cell Lysate
Reviewed Applications
Read 8 Reviews rated 4.5
using
NB100-182 in the following applications:

Publications
Read Publications using
NB100-182 in the following applications:

  • 1 publication
  • 66 publications
  • IF
    1 publication
  • IHC
    2 publications
  • 5 publications
  • IP
    5 publications
  • KO
    1 publication
  • 1 publication
  • WB
    95 publications

Reactivity Notes

This FANCD2 Antibody reacts with human and mouse FANCD2, with Primate and Canine reactivity reported in literature. Zebrafish reactivity reported in scientific literature (PMID: 30540754). Rat reactivity reported in multiple pieces of scientific literature. Kangaroo reactivity reported in scientific literature (PMID: 24982423).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FANCD2 Antibody

  • DKFZp762A223
  • FA4
  • FACD
  • FAD
  • FAD2
  • FA-D2
  • FADFAD2
  • FANCD
  • FANCD2
  • Fanconi anemia complementation group D2
  • Fanconi anemia group D2 protein
  • Fanconi anemia, complementation group D2
  • FLJ23826
  • FPN1
  • HFE4
  • IREG1
  • Protein FACD2
  • SLC11A3

Background

Fanconi Anemia (FA) is an autosomal-recessive cancer-prone disorder characterized by congenital defects, progressive bone marrow failure, increased chromosomal breakage, defective DNA repair and cellular hypersensitivity to mitomycin C. Fanconi Anemia Complementation Group D (FANCD) is comprised of two separate proteins, FANCD1 and FANCD2. MTDH, an RNA binding protein, regulates expression of FANCD2 and FANCD1 (1). FANCD2 is mapped to chromosome 3p25.3, has a theoretical molecular weight of 166 kDa and is localized in the nucleus. FANCD2 is involved in the regulation of DNA-binding transcription factor activity and DNA stability through accurate and efficient pairing of homologs during meiosis to promote repair of double-strand DNA breaks (2). Ubiquitination is required for FANCD2 to bind to chromatin. In response to DNA damage, FANCD2 is monoubiquinated to result in colocalization with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair in the nucleus and is deubiquitinated upon DNA repair completion.

References

1. Bi, J., Areecheewakul, S., Li, Y., Yang, S., Zhang, Y., Ebeid, K.,... Meng, X. (2019). MTDH/AEG-1 downregulation using pristimerin-loaded nanoparticles inhibits Fanconi anemia proteins and increases sensitivity to platinum-based chemotherapy. Gynecol Oncol, 155(2), 349-358. doi:10.1016/j.ygyno.2019.08.014

2. Balcerek, J., Jiang, J., Li, Y., Jiang, Q., Holdreith, N., Singh, B.,... Tong, W. (2018). Lnk/Sh2b3 deficiency restores hematopoietic stem cell function and genome integrity in Fancd2 deficient Fanconi anemia. Nat Commun, 9(1), 3915. doi:10.1038/s41467-018-06380-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FANCD2 Antibody (NB100-182)(171)

We have publications tested in 6 confirmed species: Human, Mouse, Avian, Canine, Primate, Zebrafish.

We have publications tested in 9 applications: ChIP, ICC/IF, IF, IHC, IHC-P, IP, KO, RNAi, WB.


Filter By Application
ChIP
(1)
ICC/IF
(66)
IF
(1)
IHC
(2)
IHC-P
(5)
IP
(5)
KO
(1)
RNAi
(1)
WB
(95)
All Applications
Filter By Species
Human
(124)
Mouse
(9)
Avian
(1)
Canine
(1)
Primate
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 171. Show All 171 Publications.
Publications using NB100-182 Applications Species
Chang E, Wells J, Tsai S et al. MRE11-RAD50-NBS1 activates Fanconi Anemia R-loop suppression at transcription-replication conflicts. Oncotarget. (WB, Human) WB Human
Gemble S, Amor-Gueret M Identification and Analysis of Different Types of UFBs Methods Mol. Biol. Aug 26 2020 [PMID: 32840780]
Olivieri M, Cho T, Alvarez-QuilOn A et al. A Genetic Map of the Response to DNA Damage in Human Cells Cell Jul 23 2020 [PMID: 32649862] (WB, KO, Human) WB, KO Human
Brannvoll A, Xue X, Kwon Y et al. The ZGRF1 Helicase Promotes Recombinational Repair of Replication-Blocking DNA Damage in Human Cells Cell Rep Jul 7 2020 [PMID: 32640219] (WB, ICC/IF, Human) WB, ICC/IF Human
Kono T, Hoover P, Poropatich K et al. Activation of DNA damage repair factors in HPV positive oropharyngeal cancers Virology Jan 1 2020 [PMID: 32560902] (IHC-P, Human) IHC-P Human
Gemble S, Buhagiar-LabarchEde G, Onclercq-Delic R et al. Topoisomerase II alpha prevents ultrafine anaphase bridges by two mechanisms Open Biol May 1 2020 [PMID: 32400307] (ICC/IF, Human) ICC/IF Human
Xing M, Zhang F, Liao H et al. Replication Stress Induces ATR/CHK1-Dependent Nonrandom Segregation of Damaged Chromosomes Mol. Cell Apr 22 2020 [PMID: 32353258]
Kreutmair S, Erlacher M, Andrieux G et al. Loss of the Fanconi anemia-associated protein NIPA causes bone marrow failure J. Clin. Invest. Jun 1 2020 [PMID: 32338640] (IHC-P, ICC/IF, Mouse, Human) IHC-P, ICC/IF Mouse, Human
Shao X, Joergensen AM, Howlett NG et al. A distinct role for recombination repair factors in an early cellular response to transcription-replication conflicts Nucleic Acids Res. Apr 24 2020 [PMID: 32329774] (ICC/IF, Avian) ICC/IF Avian
Miller HE, Gorthi A, Bassani N et al. Reconstruction of Ewing Sarcoma Developmental Context from Mass-Scale Transcriptomics Reveals Characteristics of EWSR1-FLI1 Permissibility Cancers (Basel) Apr 11 2020 [PMID: 32290418] (WB, Human) WB Human
Show All 171 Publications.

Reviews for FANCD2 Antibody (NB100-182) (8) 4.58

Average Rating: 4.5
(Based on 8 reviews)
We have 8 reviews tested in 4 species: Human, Mouse, Other, humam cell lines.

Reviews using NB100-182:
Filter by Applications
WB
(6)
IF
(1)
All Applications
Filter by Species
Human
(4)
Mouse
(1)
Other
(2)
humam cell lines
(1)
All Species
Images Ratings Applications Species Date Details
 FANCD2 NB100-182
Enlarge
5
reviewed by:
Anonymous
Mouse 01/21/2020
View

Summary

Sample TestedMouse embryonic fibroblasts
SpeciesMouse
LotN/A
Western Blot FANCD2 NB100-182
Enlarge
4
reviewed by:
Anonymous
WB humam cell lines 01/22/2018
View

Summary

ApplicationWestern Blot
Sample TestedHuman Cell lines
Specieshumam cell lines
LotR-5
Western Blot FANCD2 NB100-182
Enlarge
5
reviewed by:
Lisa Moreau
WB Human 12/15/2017
View

Summary

ApplicationWestern Blot
Sample Tested20 ug whole cell lysate
SpeciesHuman
LotR-5

Comments

CommentsThe WB was performed using 3%-8% Gel.
  5
reviewed by:
Anonymous
WB Other 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24124520
SpeciesOther

Blocking

Blocking DetailsSee PMID 24124520

Details

Detection NotesSee PMID 24124520

Comments

CommentsPublished in PMID: 24124520
  4
reviewed by:
Anonymous
IF Other 12/12/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24124520
SpeciesOther

Blocking

Blocking DetailsSee PMID 24124520

Details

Detection NotesSee PMID 24124520

Comments

CommentsPublished in PMID: 24124520
  4
reviewed by:
Anonymous
WB Human 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23318456
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23318456

Details

Detection NotesSee PMID 23318456

Comments

CommentsPublished in PMID: 23318456
Western Blot FANCD2 NB100-182
Enlarge
4
reviewed by:
Alan Yueh-Luen
WB Human 08/31/2011
View

Summary

ApplicationWestern Blot
Sample TestedHuman Cell Lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-Rabbit IgG HRP Conjugated
Western Blot FANCD2 NB100-182
Enlarge
5
reviewed by:
Wei-Ting Lu
WB Human 02/11/2011
View

Summary

ApplicationWestern Blot
Sample TestedPD20 cell line (corrected with FancD2)- whole cell extract, Sample Amount: 20 ug / lane
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 3% BSA in TBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/10000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for FANCD2 Antibody (NB100-182). (Showing 1 - 3 of 3 FAQs).

  1. Which clone is used to produce this antibody? I have some suspicion that it might be the same as the one from Abcam (ab2187). As I want to have two different staining of FANCD2, I would like to be sure that it’s not the same antibody.
    • These are polyclonal antibodies, so there will not be a clone ID associated with them. However, I can tell you that NB100-182 is the same as ab2187.
  2. We would like to know if the antibody is specific for isoform a, 1471aa (Q9BXW9) or isoform b, 1451aa (Q9BXW9-2)?
    • This antibody has been generated using human FANCD2 fusion protein (N-terminal fragment) as an immunogen. Because the isoforms a and b of FANCD2 are different in their c-terminal sequence, this antibody should not be able to differentiate the isoforms.
  3. Where does the FANCD2 Ab specifically recognizes on the protein? I see that it recognizes the N-terminus but could you tell me which AA range exactly?
    • The amino acid range for this antibody corresponds to amino acids 11-230 [Swiss-Prot #Q9BXW9].https://www.uniprot.org/uniprot/Q9BXW9#sequences

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB100-182AF405
Alexa Fluor 488 NB100-182AF488
Alexa Fluor 647 NB100-182AF647
Alexa Fluor 700 NB100-182AF700
Biotin NB100-182B
DyLight 405 NB100-182V
DyLight 488 NB100-182G
DyLight 550 NB100-182R
DyLight 650 NB100-182C
FITC NB100-182F
HRP NB100-182H

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Blogs on FANCD2.

Sample collection from mammalian culture cells for kinomic analysis
By Jamshed Arslan Pharm.D., PhD.IntroductionKinome describes kinases, and kinomics refers to the kinase signaling. Studying the effects of reagent (exogenously applied growth factor or inhibitor) on kinase activit...  Read full blog post.

FANCD2 and DNA damage repair
Fanconi anemia (FA) is a genetically inherited disorder that yields cytogenetic instability, hypersensitivity to DNA crosslinking compounds and defective DNA repair. A variety of genes have been identified within the FA pathway that are referred t...  Read full blog post.

FANCD2 (Fanconi anemia subunit D2 protein)
Fanconi anemia (FANC) is a rare, autosomal-recessive genetic disorder that is a heterogeneous cancer susceptibility condition that manifests with a wide range of symptoms such as congenital malformations, deteriorating bone marrow failure, DNA-dama...  Read full blog post.

FANCD2: A big component of the DNA repair crew
The genetic disorder known as Fanconi anemia (FANC) is a heterogeneous, autosomal-recessive cancer susceptibility condition characterized by a wide array of symptoms. These include congenital malformations, progressive bone marrow failure, DNA-damage...  Read full blog post.

FANCD2: DNA Repair and Beyond
Fanconi anemia (FANC) is a heterogenous, autosomal-recessive cancer susceptibility genetic disorder that is characterized by a wide array of symptoms, including congenital defects, progressive bone marrow failure due to DNA-damage hypersensitivity, ch...  Read full blog post.

NUP153 & 53BP1: A Novel DNA Repair Pathway
Mediating DNA damage is a crucial process, and one of the most important cellular guards against cancer. In response to DNA damage, sophisticated cellular machinery is recruited to repair the breaks, and if it fails, the cell is committed to death. De...  Read full blog post.

Fanconi Antibodies and Cancer Research
We at Novus Biologicals have an extensive antibody databasedevoted to the 13 Fanconi anaemia complementation (FANC) genes, which are involved in the recognition and repair of damaged DNA.The core complex of 8 proteins (FANCA, B, C, E, F, G, L and M)...  Read full blog post.

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Recent Reviews

4.5
5
4
4
4
3
0
2
0
1
0

Anonymous
01/21/2020
Application:
Species: Mouse

Anonymous
01/22/2018
Application: WB
Species: humam cell lines

Lisa Moreau
12/15/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol FANCD2