Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody

Images

 
Western Blot: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - Analysis of JMJD2C in HeLa nuclear extracts.
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - Antibody was tested at 1:50 in HeLa cells with Dylight 488 (green). Nuclei (Blue) were counterstained with ...read more
Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - Staining of JMJD2C in paraffin embedded mouse pancreas using DAB with hematoxylin counterstain.
Simple Western: Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody [NBP1-49600] - Simple Western lane view shows a specific band for JMJD2C in 1.0 mg/ml of HeLa lysate. This experiment was performed under ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human JMJD2C protein (within residues 450-600). [Swiss-Prot Q9H3R0]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM4C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Simple Western 1:12.5
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This JMJD2C antibody is useful for immunohistochemistry on paraffin tissues, immunocytochemistry/immunofluorescence and Western blot where a band is seen ~119 kDa in HeLa cells. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC-P, staining was observed in the nuclei of mouse pancreas. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Chromatin Immunoprecipitation reported in scientific literature (PMID: 23884959, PMID: 23129632).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
HeLa Lysate (NB800-PC9)
HeLa Lysate (NB820-59462)
NTERA-2 Lysate (NBP1-44968)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-49600 in the following applications:

Publications
Read Publications using
NBP1-49600 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody

  • EC 1.14.11
  • EC 1.14.11.-
  • GASC-1 protein
  • GASC1
  • GASC1JmjC domain-containing histone demethylation protein 3C
  • Gene amplified in squamous cell carcinoma 1 protein
  • JHDM3C
  • JMJD2C
  • JMJD2CbA146B14.1
  • jumonji domain containing 2C
  • Jumonji domain-containing protein 2C
  • KDM4C
  • KIAA0780FLJ25949
  • Lysine (K)specific Demethylase 4C
  • Lysine (K)-specific Demethylase 4C
  • lysine-specific demethylase 4C

Background

JMJD2C (Jumonji C domain-containing oxygenase D2C; also called GASC1, JHDM3C or KDM4C) belongs to JMJD2 family and is a histone demethylase for di- and trimethylated lysine 9 and 36 on histone H3 (H3K9me3/2 and H3K36me3/2). H3K9me3/2 is generally associated with transcriptional repression and heterochromatin formation, whereas, H3K36me3/2 is linked to transcriptionaly active genes where it plays a key role in suppression of incorrect transcription. JMJD2C regulates the expression of pluripotency genes including NOTCH1, NANOG, Sox2 and Pou5, and because of its dual role in modifying H3 either by removing the repressive H3K9me3/2 or the active H3K36me3/2 factor, JMJD2C has been considered a fine-tuning regulator of gene expression in normal development/differentiation as well as in carcinogenic progression. JMJD2C is overexpressed in aggressive, basal-like breast cancers compared with non basal-like breast cancers, and its overexpression has been shown to cause transformation of immortalized, non-transformed mammary epithelial cells and to regulate expression of genes responsible for stem cell phenotype/self-renewal in breast cancer cells. Moreover oncogenic role of JMJD2C has been documented in prostate cancer where it enhances the transcription of AR-dependent genes and cell proliferation by interaction with ligand-bound AR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (NBP1-49600)(3)

Review for Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (NBP1-49600) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-49600:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C NBP1-49600
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reviewed by:
Anonymous
WB Human 05/03/2012
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Summary

ApplicationWestern Blot
Sample TestedHeLa lysates
SpeciesHuman

Blocking

Blocking Details5% milk

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG-HRP
Secondary Concentration1:5000

Details

Detection NotesECL plus

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibody (NBP1-49600). (Showing 1 - 2 of 2 FAQs).

  1. I am interested in the KDM4cJMJD2c antibody NBP1-49600. According to their website, it is used against human and murine JMJD2c protein. In addition, there is an image of an Immunhisto (Mouse pancreas).We need the antibody for the main part for Western blots with mouse protein. Are there positive reviews about it? Did they specify the reactivity? How specific does it recognize the protein? Does it recognize the protein properly? For an antibody of a different company, we already had the problem that mouse was specified on their datasheet but in the end wasn't recognized.
    • This antibody, being a polyclonal does recognize both the human and mouse proteins. It has been shown to work much better on the native mouse proteins in IHC than in Western blot. It does recognize mouse JMJD2C in Western blot but we have seen that it does give some background staining. Regardless, we have a 100% guarantee on all of our products for the applications and species listed on our datasheet, so we will guarantee this for Western blot on mouse samples.
  2. How has this antibody been tested for reactivity with mouse other than immunohistochemistry?
    • We do not have data for NBP1-49600 with mouse other than in IHC-P. However, if you use NBP1-49600 to analyze your mouse samples in any of the validated applications (those stated on the datasheet) for this antibody, you would be covered by our Novus Guarantee, which means that if you did have any issues, we would resolve them.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49600AF405
Alexa Fluor 488 Labeled NBP1-49600AF488
Alexa Fluor 647 Labeled NBP1-49600AF647
Alexa Fluor 700 Labeled NBP1-49600AF700
Biotin Labeled NBP1-49600B
DyLight 405 Labeled NBP1-49600V
DyLight 488 Labeled NBP1-49600G
DyLight 550 Labeled NBP1-49600R
DyLight 650 Labeled NBP1-49600C
FITC Labeled NBP1-49600F
HRP Labeled NBP1-49600H

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Recent Reviews

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1
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1
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Anonymous
05/03/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol KDM4C
Entrez
Uniprot