Leptin/OB Antibody

Western Blot: Leptin/OB Antibody [NB300-611] - Analysis of leptin in mouse 3T3-L1.

Immunohistochemistry-Frozen: Leptin/OB Antibody [NB300-611] - Staining of Leptin in sheep brain.

• Reactivity: Hu, Mu, Rt, Po, Av, Sh
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 2 mg/ml

Leptin/OB Antibody Summary

• Description: Novus Biologicals Rabbit Leptin/OB Antibody (NB300-611) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Leptin/OB Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin.
• Localization: Secreted
• Specificity: Leptin
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LEP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Frozen 1:50 - 1:200
  • Western Blot 1:200 - 1:1000
• Application Notes: WB: Detects an approx. 16 kDa protein representing leptin from 3T3-L1 cell extract. IHC: Staining of leptin in sheep brain with NB300-611 results in staining of the ventromedial hypothalamus.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23270329). Human reactivity reported in scientific literature (PMID: 21983012). Porcine reactivity reported in scientific literature (PMID: 18413648). Avian reactivity reported in scientific literature (PMID: 16730725).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Leptin/OB Antibody

• FLJ94114
• LEP
• leptin (murine obesity homolog)
• leptin (obesity homolog, mouse)
• Leptin
• OB
• Obese protein
• obese, mouse, homolog of
• Obesity factor
• OBOBS

Background

Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure. Ob gene knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Leptin/OB Antibody (NB300-611)(1)

Publication using NB300-611

• Kuramoto K, Kim YJ, Hong JH, He C The autophagy protein Becn1 improves insulin sensitivity by promoting adiponectin secretion via exocyst binding Cell reports 2021-05-25 [PMID: 34038729]

Bioinformatics

• Gene Symbol: LEP
• Entrez:
  • 3952(Human)
  • 16846(Mouse)
  • 25608(Rat)
• Uniprot:
  • P41160(Mouse)