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498-OB) was serially diluted and captured by Goat Anti-Mouse Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF498) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF498) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
498-OB) was serially diluted and captured by Goat Anti-Mouse Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF498) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF498) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
398-LP) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Leptin/OB (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Human Leptin/OB Monoclonal Antibody (Catalog # MAB398). The ND50 is typically 0.4-1.6 µg/mL." class="big_lightbox" target="_blank">
398-LP) was serially diluted and captured by Mouse Anti-Human Leptin/OB Monoclonal Antibody (Catalog # MAB398) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Leptin/OB Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox" target="_blank">
398-LP), Recombinant Mouse Leptin/OB (Catalog # 498-OB) and Recombinant Rat Leptin/OB (Catalog # 598-LP). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Leptin/OB at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox" target="_blank">
398-LP) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Leptin/OB (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398). The ND50 is typically 0.5-3.0 µg/mL." class="big_lightbox" target="_blank">
Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure. Ob gene knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.
![Western Blot Leptin/OB Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Leptin_AF398_Western_Blot_21838.jpg)
![Sandwich ELISA Leptin/OB Antibody Pair [HRP]](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79538-img0001.jpg)
![N/A Leptin/OB [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_Leptin_DLP00_ELISA_749.jpg)
![N/A Leptin/OB [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Leptin_DY398_ELISA_1995.jpg)
![N/A Leptin/OB [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Leptin_DY498_ELISA_2068.jpg)
![SEC-MALS Leptin/OB [Unconjugated]](http://images.novusbio.com/fullsize/498-ob_recombinant-mouse-leptin-protein-cf-sec-mals-81202612141.jpg)
![SEC-MALS Leptin/OB [Unconjugated]](http://images.novusbio.com/fullsize/398-lp_recombinant-human-leptin-protein-cf-sec-mals-612026133924.jpg)
![Immunohistochemistry Leptin/OB Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Leptin_AF398_Immunohistochemistry_17359.jpg)
![Neutralization Leptin/OB Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Leptin_AF398_Block_Neutralize_8871.jpg)
![Immunohistochemistry Leptin/OB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af398_human-leptin-affinity-purified-polyclonal-ab-immunohistochemistry-1212202592089.jpg)
![Immunohistochemistry Leptin/OB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af398_human-leptin-affinity-purified-polyclonal-ab-immunohistochemistry-121220258541124.jpg)
![Immunocytochemistry/ Immunofluorescence Leptin/OB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af398_human-leptin-affinity-purified-polyclonal-ab-immunocytochemistry-immunofluorescence-121220259161715.jpg)
![Immunocytochemistry/ Immunofluorescence Leptin/OB Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af398_human-leptin-affinity-purified-polyclonal-ab-immunocytochemistry-immunofluorescence-121220259231617.jpg)
![N/A Leptin/OB [HRP]](http://images.novusbio.com/fullsize/elisa/Leptin_DLP00_ELISA_134.jpg)
![Bioactivity Leptin/OB [Unconjugated]](http://images.novusbio.com/fullsize/protein/Leptin_398-LP_432.jpg)
![SDS-Page Leptin/OB [Unconjugated]](http://images.novusbio.com/fullsize/protein/Leptin_398-LP_431.jpg)
