Leptin R Antibody

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Leptin R was detected in perfusion fixed frozen sections of rat brain using 15 µg/mL Goat Anti-Mouse Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF497) overnight at 4 °C. Tissue was stained with ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Leptin R Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Leptin R
Ala20-Gly839
Accession # Q3US58
Specificity
Detects mouse Leptin R in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Leptin R is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LEPR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
Publications
Read Publications using
AF497 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Leptin R Antibody

  • B219
  • CD295 antigen
  • CD295
  • DB
  • DKFZp686B1731
  • huB219
  • LEPR
  • LEP-R
  • Leptin R
  • leptin receptor
  • LeptinR
  • OB R
  • OB receptor
  • OB-R
  • OBRCD295

Background

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. Circulating soluble OB-R, complexed to leptin, has been detected in mouse serum. Serum soluble OB-R levels have been shown to increase during pregnancy. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R has recently been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype.

Mouse OB-R long form encodes a 1162 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 817 aa residue extracellular domain, a 21 aa residue transmembrane domain, and a 302 aa residue cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant murine soluble OB-R has been shown to bind leptin with high affinity and is a potent leptin antagonist.

  1. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  2. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
  3. Lee, J.I. and J.M. Friedman (1996) Nature 379:632.
  4. Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
  5. Gavrilova, O. et al. (1997) J. Biol. Chem. 272:30546.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Leptin R Antibody (AF497)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: Flow, ICC, IHC, IHC Frozen, IHC-Fr, IP, WB.


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Flow
(2)
ICC
(2)
IHC
(4)
IHC Frozen
(1)
IHC-Fr
(1)
IP
(1)
WB
(2)
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Human
(3)
Mouse
(10)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF497 Applications Species
C Nozaki, E Nent, A Bilkei-Gor, A Zimmer Involvement of leptin signaling in the development of cannabinoid CB2 receptor-dependent mirror image pain Sci Rep, 2018;8(1):10827. 2018 [PMID: 30018366] (IHC,, Mouse) IHC Mouse
X Wang, L Xie, J Crane, G Zhen, F Li, P Yang, M Gao, R Deng, Y Wang, X Jia, C Fan, M Wan, X Cao Aberrant TGF-? activation in bone tendon insertion induces enthesopathy-like disease J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29355842] (IHC,, Mouse) IHC Mouse
DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2018;15(1):39-46. 2018 [PMID: 29320487] (IHC,, Mouse) IHC Mouse
C Li, Y Chai, L Wang, B Gao, H Chen, P Gao, FQ Zhou, X Luo, JL Crane, B Yu, X Cao, M Wan Programmed cell senescence in skeleton during late puberty Nat Commun, 2017;8(1):1312. 2017 [PMID: 29101351] (Flow,, Mouse) Flow Mouse
KT Chang, YL Lin, CT Lin, MJ Tsai, WC Huang, YH Shih, YY Lee, H Cheng, MC Huang Data on the expression of leptin and leptin receptor in the dorsal root ganglion and spinal cord after preganglionic cervical root avulsion Data Brief, 2017;15(0):567-572. 2017 [PMID: 29071294] (IHC-Fr,, Mouse) IHC-Fr Mouse
R Martín, C Cordova, B Gutiérrez, M Hernández, ML Nieto A dangerous liaison: Leptin and sPLA2-IIA join forces to induce proliferation and migration of astrocytoma cells PLoS ONE, 2017;12(3):e0170675. 2017 [PMID: 28249041] (Flow,, Human) Flow Human
N Asada, Y Kunisaki, H Pierce, Z Wang, NF Fernandez, A Birbrair, A Ma'ayan, PS Frenette Differential cytokine contributions of perivascular haematopoietic stem cell niches Nat. Cell Biol, 2017;19(3):214-223. 2017 [PMID: 28218906] (IHC,, Mouse) IHC Mouse
Stratigopoulos G, Martin Carli J, O'Day D, Wang L, Leduc C, Lanzano P, Chung W, Rosenbaum M, Egli D, Doherty D, Leibel R Hypomorphism for RPGRIP1L, a ciliary gene vicinal to the FTO locus, causes increased adiposity in mice. Cell Metab, 2014;19(5):767-79. 2014 [PMID: 24807221] (IP,, Human) IP Human
Verkerke H, Naylor C, Zabeau L, Tavernier J, Petri W, Marie C Kinetics of leptin binding to the Q223R leptin receptor. PLoS ONE, 2014;9(4):e94843. 2014 [PMID: 24743494] (WB,, Human) WB Human
Pitts, Matthew, Reeves, Mariclai, Hashimoto, Ann C, Ogawa, Ashley, Kremer, Penny, Seale, Lucia A, Berry, Marla J Deletion of selenoprotein M leads to obesity without cognitive deficits. J Biol Chem, 2013;288(36):26121-34. 2013 [PMID: 23880772] (IHC Frozen,, Mouse) IHC Frozen Mouse
Show All 13 Publications.

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Bioinformatics

Gene Symbol LEPR
Entrez
Uniprot