Leptin R Antibody


Leptin R was detected in perfusion fixed frozen sections of rat brain using 15 µg/mL Goat Anti-Mouse Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF497) overnight at 4 °C. Tissue was stained with ...read more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready

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Leptin R Antibody Summary

Mouse myeloma cell line NS0-derived recombinant mouse Leptin R
Accession # Q3US58
Detects mouse Leptin R in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Leptin R is observed.
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Read Publications using
AF497 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Leptin R Antibody

  • B219
  • CD295 antigen
  • CD295
  • DB
  • DKFZp686B1731
  • huB219
  • LEPR
  • LEP-R
  • Leptin R
  • leptin receptor
  • LeptinR
  • OB R
  • OB receptor
  • OB-R
  • OBRCD295


Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. Circulating soluble OB-R, complexed to leptin, has been detected in mouse serum. Serum soluble OB-R levels have been shown to increase during pregnancy. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R has recently been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype.

Mouse OB-R long form encodes a 1162 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 817 aa residue extracellular domain, a 21 aa residue transmembrane domain, and a 302 aa residue cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant murine soluble OB-R has been shown to bind leptin with high affinity and is a potent leptin antagonist.

  1. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  2. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
  3. Lee, J.I. and J.M. Friedman (1996) Nature 379:632.
  4. Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
  5. Gavrilova, O. et al. (1997) J. Biol. Chem. 272:30546.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Leptin R Antibody (AF497)(22)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, Immunoprecipitation, Western Blot.

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Flow Cytometry
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Transgenic Mouse
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Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using AF497 Applications Species
S Yuan, KS Stewart, Y Yang, MD Abdusselam, SM Parigi, TY Feinberg, K Tumaneng, H Yang, JM Levorse, L Polak, D Ng, E Fuchs Ras drives malignancy through stem cell crosstalk with the microenvironment Nature, 2022-11-30;0(0):. 2022-11-30 [PMID: 36450983] (IHC, Mouse) IHC Mouse
W Zhang, Z Xu, X Hao, T He, J Li, Y Shen, K Liu, Y Gao, J Liu, D Edwards, AM Muscarella, L Wu, L Yu, L Xu, X Chen, YH Wu, IL Bado, Y Ding, S Aguirre, H Wang, Z Gugala, RL Satcher, ST Wong, XH Zhang Bone metastasis initiation is coupled with bone remodeling through osteogenic differentiation of NG2+ cells Cancer Discovery, 2023-02-06;0(0):. 2023-02-06 [PMID: 36287038] (IHC, Transgenic Mouse) IHC Transgenic Mouse
EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6. 2022-10-21 [PMID: 36272401] (Flow Cytometry, IHC, Mouse) Flow Cytometry, IHC Mouse
CH Lee, DK Song, CB Park, J Choi, GM Kang, SH Shin, I Kwon, S Park, S Kim, JY Kim, H Dugu, JW Park, JH Choi, SH Min, JW Sohn, MS Kim Primary cilia mediate early life programming of adiposity through lysosomal regulation in the developing mouse hypothalamus Nat Commun, 2020-11-13;11(1):5772. 2020-11-13 [PMID: 33188191] (ICC, Mouse) ICC Mouse
M Saçma, J Pospiech, R Bogeska, W de Back, JP Mallm, V Sakk, K Soller, G Marka, A Vollmer, R Karns, N Cabezas-Wa, A Trumpp, S Méndez-Fer, MD Milsom, MA Mulaw, H Geiger, MC Florian Haematopoietic stem cells in perisinusoidal niches are protected from ageing Nat. Cell Biol., 2019-11-04;21(11):1309-1320. 2019-11-04 [PMID: 31685996] (ICC, Mouse) ICC Mouse
HA Himburg, M Roos, T Fang, Y Zhang, CM Termini, L Schlussel, MM Kim, A Pang, J Kan, L Zhao, H Suh, JP Sasine, G Sapparapu, PM Bowers, G Schiller, JP Chute Chronic myelogenous leukemia stem cells require cell-autonomous pleiotrophin signaling J. Clin. Invest., 2020-01-02;0(0):. 2020-01-02 [PMID: 31613796] (IHC-Fr, Mouse) IHC-Fr Mouse
N Severe, NM Karabacak, K Gustafsson, N Baryawno, G Courties, Y Kfoury, KD Kokkaliari, C Rhee, D Lee, EW Scadden, JE Garcia-Rob, T Brouse, M Nahrendorf, M Toner, DT Scadden Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping Cell Stem Cell, 2019-07-03;0(0):. 2019-07-03 [PMID: 31279774] (IHC, Mouse) IHC Mouse
F Piattini, C Le Foll, J Kisielow, E Rosenwald, P Nielsen, T Lutz, C Schneider, M Kopf A spontaneous leptin receptor point mutation causes obesity and differentially affects leptin signaling in hypothalamic nuclei resulting in metabolic dysfunctions distinct from db/db mice Mol Metab, 2019-04-25;0(0):. 2019-04-25 [PMID: 31076350] (Flow Cytometry, Mouse) Flow Cytometry Mouse
BJ Frisch, CM Hoffman, SE Latchney, MW LaMere, J Myers, J Ashton, AJ Li, J Saunders, J Palis, AS Perkins, A McCabe, JN Smith, KE McGrath, F Rivera-Esc, A McDavid, JL Liesveld, VA Korshunov, MR Elliott, KC MacNamara, MW Becker, LM Calvi Aged marrow macrophages expand platelet-biased hematopoietic stem cells via Interleukin1B JCI Insight, 2019-04-18;5(0):. 2019-04-18 [PMID: 30998506] (IHC, Mouse) IHC Mouse
C Nozaki, E Nent, A Bilkei-Gor, A Zimmer Involvement of leptin signaling in the development of cannabinoid CB2 receptor-dependent mirror image pain Sci Rep, 2018-07-17;8(1):10827. 2018-07-17 [PMID: 30018366] (IHC, Mouse) IHC Mouse
Show All 22 Publications.

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Gene Symbol LEPR