Leptin R Antibody

Leptin R was detected in perfusion fixed frozen sections of rat brain using 15 µg/mL Goat Anti-Mouse Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF497) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in the choroid plexus. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Leptin R Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Leptin R
  Ala20-Gly839
  Accession # Q3US58
• Specificity: Detects mouse Leptin R in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Leptin R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LEPR
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Leptin R Antibody

• B219
• CD295 antigen
• CD295
• DB
• DKFZp686B1731
• huB219
• LEPR
• LEP-R
• Leptin R
• leptin receptor
• LeptinR
• OB R
• OB receptor
• OB-R
• OBRCD295

Background

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-R_L) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-R_s) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. Circulating soluble OB-R, complexed to leptin, has been detected in mouse serum. Serum soluble OB-R levels have been shown to increase during pregnancy. OB-R_L transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-R_s transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R has recently been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype.

Mouse OB-R long form encodes a 1162 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 817 aa residue extracellular domain, a 21 aa residue transmembrane domain, and a 302 aa residue cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant murine soluble OB-R has been shown to bind leptin with high affinity and is a potent leptin antagonist.

1. Tartaglia, L.A. et al. (1995) Cell 83:1263.
2. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
3. Lee, J.I. and J.M. Friedman (1996) Nature 379:632.
4. Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
5. Gavrilova, O. et al. (1997) J. Biol. Chem. 272:30546.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Leptin R Antibody (AF497)(19)

Publications using AF497

• CH Lee, DK Song, CB Park, J Choi, GM Kang, SH Shin, I Kwon, S Park, S Kim, JY Kim, H Dugu, JW Park, JH Choi, SH Min, JW Sohn, MS Kim Primary cilia mediate early life programming of adiposity through lysosomal regulation in the developing mouse hypothalamus Nat Commun, 2020;11(1):5772. 2020 [PMID: 33188191] (ICC, Mouse)
• M Saçma, J Pospiech, R Bogeska, W de Back, JP Mallm, V Sakk, K Soller, G Marka, A Vollmer, R Karns, N Cabezas-Wa, A Trumpp, S Méndez-Fer, MD Milsom, MA Mulaw, H Geiger, MC Florian Haematopoietic stem cells in perisinusoidal niches are protected from ageing Nat. Cell Biol., 2019;21(11):1309-1320. 2019 [PMID: 31685996] (ICC, Mouse)
• HA Himburg, M Roos, T Fang, Y Zhang, CM Termini, L Schlussel, MM Kim, A Pang, J Kan, L Zhao, H Suh, JP Sasine, G Sapparapu, PM Bowers, G Schiller, JP Chute Chronic myelogenous leukemia stem cells require cell-autonomous pleiotrophin signaling J. Clin. Invest., 2019;0(0):. 2019 [PMID: 31613796] (IHC-Fr, Mouse)
• N Severe, NM Karabacak, K Gustafsson, N Baryawno, G Courties, Y Kfoury, KD Kokkaliari, C Rhee, D Lee, EW Scadden, JE Garcia-Rob, T Brouse, M Nahrendorf, M Toner, DT Scadden Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping Cell Stem Cell, 2019;0(0):. 2019 [PMID: 31279774] (IHC, Mouse)
• F Piattini, C Le Foll, J Kisielow, E Rosenwald, P Nielsen, T Lutz, C Schneider, M Kopf A spontaneous leptin receptor point mutation causes obesity and differentially affects leptin signaling in hypothalamic nuclei resulting in metabolic dysfunctions distinct from db/db mice Mol Metab, 2019;0(0):. 2019 [PMID: 31076350] (Flow Cytometry, Mouse)
• BJ Frisch, CM Hoffman, SE Latchney, MW LaMere, J Myers, J Ashton, AJ Li, J Saunders, J Palis, AS Perkins, A McCabe, JN Smith, KE McGrath, F Rivera-Esc, A McDavid, JL Liesveld, VA Korshunov, MR Elliott, KC MacNamara, MW Becker, LM Calvi Aged marrow macrophages expand platelet-biased hematopoietic stem cells via Interleukin1B JCI Insight, 2019;5(0):. 2019 [PMID: 30998506] (IHC, Mouse)
• C Nozaki, E Nent, A Bilkei-Gor, A Zimmer Involvement of leptin signaling in the development of cannabinoid CB2 receptor-dependent mirror image pain Sci Rep, 2018;8(1):10827. 2018 [PMID: 30018366] (IHC, Mouse)
• X Wang, L Xie, J Crane, G Zhen, F Li, P Yang, M Gao, R Deng, Y Wang, X Jia, C Fan, M Wan, X Cao Aberrant TGF-? activation in bone tendon insertion induces enthesopathy-like disease J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29355842] (IHC, Mouse)
• DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2018;15(1):39-46. 2018 [PMID: 29320487] (IHC, Mouse)
• C Li, Y Chai, L Wang, B Gao, H Chen, P Gao, FQ Zhou, X Luo, JL Crane, B Yu, X Cao, M Wan Programmed cell senescence in skeleton during late puberty Nat Commun, 2017;8(1):1312. 2017 [PMID: 29101351] (Flow Cytometry, Mouse)
• KT Chang, YL Lin, CT Lin, MJ Tsai, WC Huang, YH Shih, YY Lee, H Cheng, MC Huang Data on the expression of leptin and leptin receptor in the dorsal root ganglion and spinal cord after preganglionic cervical root avulsion Data Brief, 2017;15(0):567-572. 2017 [PMID: 29071294] (IHC-Fr, Mouse)
• R Martín, C Cordova, B Gutiérrez, M Hernández, ML Nieto A dangerous liaison: Leptin and sPLA2-IIA join forces to induce proliferation and migration of astrocytoma cells PLoS ONE, 2017;12(3):e0170675. 2017 [PMID: 28249041] (Flow Cytometry, Human)
• N Asada, Y Kunisaki, H Pierce, Z Wang, NF Fernandez, A Birbrair, A Ma'ayan, PS Frenette Differential cytokine contributions of perivascular haematopoietic stem cell niches Nat. Cell Biol, 2017;19(3):214-223. 2017 [PMID: 28218906] (IHC, Mouse)
• Stratigopoulos G, Martin Carli J, O'Day D, Wang L, Leduc C, Lanzano P, Chung W, Rosenbaum M, Egli D, Doherty D, Leibel R Hypomorphism for RPGRIP1L, a ciliary gene vicinal to the FTO locus, causes increased adiposity in mice. Cell Metab, 2014;19(5):767-79. 2014 [PMID: 24807221] (Immunoprecipitation, Human)
• Verkerke H, Naylor C, Zabeau L, Tavernier J, Petri W, Marie C Kinetics of leptin binding to the Q223R leptin receptor. PLoS ONE, 2014;9(4):e94843. 2014 [PMID: 24743494] (Western Blot, Human)
• Pitts , Matthew, Reeves , Mariclai, Hashimoto , Ann C, Ogawa , Ashley, Kremer , Penny, Seale , Lucia A, Berry , Marla J Deletion of selenoprotein M leads to obesity without cognitive deficits. J Biol Chem, 2013;288(36):26121-34. 2013 [PMID: 23880772] (IHC-Fr, Mouse)
• Kiguchi N, Maeda T, Kobayashi Y, Fukazawa Y, Kishioka S Leptin enhances CC-chemokine ligand expression in cultured murine macrophage. Biochem. Biophys. Res. Commun., 2009;384(3):311-5. 2009 [PMID: 19409880] (ICC, Mouse)
• Tu H, Kastin AJ, Hsuchou H, Pan W Soluble receptor inhibits leptin transport. J. Cell. Physiol., 2008;214(2):301-5. 2008 [PMID: 17620316] (ICC, Mouse)
• Cohen SE, Kokkotou E, Biddinger SB, Kondo T, Gebhardt R, Kratzsch J, Mantzoros CS, Kahn CR High circulating leptin receptors with normal leptin sensitivity in liver-specific insulin receptor knock-out (LIRKO) mice. J. Biol. Chem., 2007;282(32):23672-8. 2007 [PMID: 17556363] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: LEPR
• Uniprot:
  • Q3US58()