Human Total Adiponectin/Acrp30 Quantikine ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Catalog# & Conjugate Size Price

Human Total Adiponectin/Acrp30 Quantikine ELISA Kit Summary

Background
The Quantikine Human Total Adiponectin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure total (low, middle, and high molecular weight) human Adiponectin in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Adiponectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Adiponectin showed... linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Adiponectin.
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Specificity
Natural and recombinant human total Adiponectin (low, middle, and high molecular weight)
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
ADIPOQ

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
DRP300 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Total Adiponectin/Acrp30 Quantikine ELISA Kit

  • ACDC
  • Acrp30
  • ACRP30ADPN
  • adipocyte, C1Q and collagen domain containing
  • Adiponectin
  • adiponectin, C1Q and collagen domain containing
  • AdipoQ
  • ADIPQTL1
  • ApM1
  • apM-1
  • APM1APM-1
  • C1q and collagen domain-containing protein
  • GBP28
  • GBP28apM1
  • Gelatin-binding protein

Background

Adiponectin, alternately named Adipocyte complement-related protein of 30 kDa (Acrp30), adipoQ, adipose most abundant gene transcript 1 (apM1), and gelatin-binding protein of 28 kDa (GBP28), is an adipocyte-specific, secreted protein with potential roles in glucose and lipid homeostasis. Circulating Adiponectin levels are high, accounting for approximately 0.01% of total plasma protein (1-4). Adiponectin contains a modular structure that includes an N-terminal collagen-like domain followed by a C-terminal globular domain with significant sequence and structural resemblance to the complement factor C1q (1, 5, 6). Although they share little sequence identity, similar threedimensional structure and certain conserved amino acid residues suggest an evolutionary link between the C1q-like domain of Adiponectin and members of the TNF superfamily (7). Adiponectin assembles into different complexes including trimers (low molecular weight), hexamers (middle molecular weight), and higher order oligomeric structures (high molecular weight) that may affect biological activity (1, 7, 8). Adiponectin is induced during adipocyte differentiation and its secretion is stimulated by insulin (1, 9). Two receptors for Adiponectin, termed AdipoR1 and AdipoR2, have been cloned (10). Although functionally distinct from G-protein-coupled receptors, the genes encode predicted proteins containing 7 transmembrane domains. AdipoR1 is highly expressed in skeletal muscle, while AdipoR2 is primarily found in hepatic tissues. 
Injection of Adiponectin into non-obese diabetic mice leads to an insulin-independent decrease in glucose levels (11). This is likely due to insulin-sensitizing effects involving Adiponectin regulation of triglyceride metabolism (11). A truncated form of Adiponectin (gAdiponectin) containing only the C-terminal globular domain has been identified in the blood, and recombinant gAdiponectin has been shown to regulate weight reduction as well as free fatty acid oxidation in mouse muscle and liver (2, 12). The full-length recombinant Adiponectin protein is apparently less potent at mediating these effects (2, 12). The mechanism underlying the role of Adiponectin in lipid oxidation may involve the regulation of expression or activity of proteins associated with triglyceride metabolism including CD36, acyl CoA oxidase, AMPK, and PPAR gamma (12-14). 
Although Adiponectin-regulation of glucose and lipid metabolism in humans is less clear, similar mechanisms may also be in place (15). A negative correlation between obesity and circulating Adiponectin has been well established (6, 16, 17), and Adiponectin levels increase concomitantly with weight loss (18). Decreased Adiponectin levels are associated with insulin resistance and hyperinsulinemia, and patients with type-2 diabetes are reported to exhibit decreased circulating Adiponectin (19, 20). Thiazolidinediones, a class of insulin-sensitizing, anti-diabetic drugs, elevate Adiponectin in insulin-resistant patients (21). In addition, high Adiponectin levels are associated with a reduced risk of type-2 diabetes (22). Using magnetic resonance spectroscopy it has been demonstrated that intracellular lipid content in human muscle negatively correlates with Adiponectin levels, potentially due to Adiponectin-induced fatty acid oxidation (15). 
Adiponectin may also play anti-atherogenic and anti-inflammatory roles. Adiponectin plasma levels are decreased in patients with coronary artery disease (20). Furthermore, neointimal thickening of damaged arteries is exacerbated in Adiponectin-deficient mice and is inhibited by exogenous Adiponectin (23). Adiponectin inhibits endothelial cell expression of adhesion molecules in vitro, suppressing the attachment of monocytes (24). In addition, Adiponectin negatively regulates myelomonocytic progenitor cell growth and TNF-alpha production in macrophages (25, 26).
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Adiponectin/Acrp30 (DRP300)(176)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Chlorocebus aethiops (African Green Monkey).

We have publications tested in 1 application: ELISA Capture.


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Human
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Mouse
(2)
Primate - Chlorocebus aethiops (African Green Monkey)
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Showing Publications 1 - 10 of 176. Show All 176 Publications.
Publications using DRP300 Applications Species
McNaughton, BA;Burrows, K;Choquette, E;Poplin, T;Kuplicki, R;Paulus, MP;Ironside, M;Stewart, JL; Impaired eating behaviors but intact metabolic hormone levels in individuals with major depressive disorder and generalized anxiety disorder Journal of psychiatric research 2023-10-26 [PMID: 37918032] (Human) Human
Gu, Y;Avolio, E;Alvino, VV;Thomas, AC;Herman, A;Miller, PJ;Sullivan, N;Faulkner, A;Madeddu, P; The tyrosine kinase inhibitor Dasatinib reduces cardiac steatosis and fibrosis in obese, type 2 diabetic mice Cardiovascular diabetology 2023-08-17 [PMID: 37592236] (Human) Human
Sparks, L;Whytock, K;Divoux, A;Sun, Y;Pino, M;Yu, G;Smith, S;Walsh, M; A single nuclei atlas of aging human abdominal subcutaneous white adipose tissue Research square 2023-07-13 [PMID: 37503028] (Human) Human
Burrows, K;McNaughton, BA;Figueroa-Hall, LK;Spechler, PA;Kuplicki, R;Victor, TA;Aupperle, R;Khalsa, SS;Savitz, JB;Teague, TK;Paulus, MP;Stewart, JL; Elevated serum leptin is associated with attenuated reward anticipation in major depressive disorder independent of peripheral C-reactive protein levels Scientific reports 2023-07-13 [PMID: 37443383] (Human) Human
Pletikosic, I;Marasovic Krstulovic, D;Bakovic, D;Susilovic Grabovac, Z;Tandara, L;Martinovic Kaliterna, D; Association of inflammatory biomarkers and disease activity with subclinical myocardial dysfunction in psoriatic arthritis Scientific reports 2023-06-26 [PMID: 37365233] (Human) Human
Policastro, V;Righelli, D;Ravà, L;Vernocchi, P;Bianchi, M;Vallone, C;Signore, F;Manco, M; Dietary Fatty Acids Contribute to Maintaining the Balance between Pro-Inflammatory and Anti-Inflammatory Responses during Pregnancy Nutrients 2023-05-23 [PMID: 37299395] (Human) Human
Bilak, JM;Yeo, JL;Gulsin, GS;Marsh, AM;Sian, M;Dattani, A;Ayton, SL;Parke, KS;Bain, M;Pang, W;Boulos, S;Pierre, TGS;Davies, MJ;Yates, T;McCann, GP;Brady, EM; Impact of the Remission of Type 2 Diabetes on Cardiovascular Structure and Function, Exercise Capacity and Risk Profile: A Propensity Matched Analysis Journal of cardiovascular development and disease 2023-04-24 [PMID: 37233158] (Human) Human
Coimbra, S;Catarino, C;Sameiro Faria, M;Nunes, JPL;Rocha, S;Valente, MJ;Rocha-Pereira, P;Bronze-da-Rocha, E;Bettencourt, N;Beco, A;Marques, SHM;Oliveira, JG;Madureira, J;Fernandes, JC;Miranda, V;Belo, L;Santos-Silva, A; The Association of Leptin with Left Ventricular Hypertrophy in End-Stage Kidney Disease Patients on Dialysis Biomedicines 2023-03-27 [PMID: 37189644] (Human) Human
LV Trevor, K Riches-Sum, AL Mahajan, MJ Thornton Stromal Vascular Fraction Cells from Individuals Who Have Previously Undergone Radiotherapy Retain Their Pro-Wound Healing Properties Journal of Clinical Medicine, 2023-03-04;12(5):. 2023-03-04 [PMID: 36902839] (Human) Human
M Kanazashi, T Iida, R Nakanishi, M Tanaka, H Ikeda, N Takamiya, N Maeshige, H Kondo, T Nishigami, T Harada, H Fujino Brazilian Propolis Intake Decreases Body Fat Mass and Oxidative Stress in Community-Dwelling Elderly Females: A Randomized Placebo-Controlled Trial Nutrients, 2023-01-11;15(2):. 2023-01-11 [PMID: 36678234] (Human) Human
Show All 176 Publications.

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Bioinformatics

Gene Symbol ADIPOQ