Human Total Adiponectin/Acrp30 Quantikine ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

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Catalog# & Conjugate Size Price

Human Total Adiponectin/Acrp30 Quantikine ELISA Kit Summary

Background
The Quantikine Human Total Adiponectin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure total (low, middle, and high molecular weight) human Adiponectin in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Adiponectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Adiponectin showed... linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Adiponectin.
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Specificity
Natural and recombinant human total Adiponectin (low, middle, and high molecular weight)
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
ADIPOQ

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
DRP300 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Total Adiponectin/Acrp30 Quantikine ELISA Kit

  • ACDC
  • Acrp30
  • ACRP30ADPN
  • adipocyte, C1Q and collagen domain containing
  • Adiponectin
  • adiponectin, C1Q and collagen domain containing
  • AdipoQ
  • ADIPQTL1
  • ApM1
  • apM-1
  • APM1APM-1
  • C1q and collagen domain-containing protein
  • GBP28
  • GBP28apM1
  • Gelatin-binding protein

Background

Adiponectin, alternately named Adipocyte complement-related protein of 30 kDa (Acrp30), adipoQ, adipose most abundant gene transcript 1 (apM1), and gelatin-binding protein of 28 kDa (GBP28), is an adipocyte-specific, secreted protein with potential roles in glucose and lipid homeostasis. Circulating Adiponectin levels are high, accounting for approximately 0.01% of total plasma protein (1-4). Adiponectin contains a modular structure that includes an N-terminal collagen-like domain followed by a C-terminal globular domain with significant sequence and structural resemblance to the complement factor C1q (1, 5, 6). Although they share little sequence identity, similar threedimensional structure and certain conserved amino acid residues suggest an evolutionary link between the C1q-like domain of Adiponectin and members of the TNF superfamily (7). Adiponectin assembles into different complexes including trimers (low molecular weight), hexamers (middle molecular weight), and higher order oligomeric structures (high molecular weight) that may affect biological activity (1, 7, 8). Adiponectin is induced during adipocyte differentiation and its secretion is stimulated by insulin (1, 9). Two receptors for Adiponectin, termed AdipoR1 and AdipoR2, have been cloned (10). Although functionally distinct from G-protein-coupled receptors, the genes encode predicted proteins containing 7 transmembrane domains. AdipoR1 is highly expressed in skeletal muscle, while AdipoR2 is primarily found in hepatic tissues. 
Injection of Adiponectin into non-obese diabetic mice leads to an insulin-independent decrease in glucose levels (11). This is likely due to insulin-sensitizing effects involving Adiponectin regulation of triglyceride metabolism (11). A truncated form of Adiponectin (gAdiponectin) containing only the C-terminal globular domain has been identified in the blood, and recombinant gAdiponectin has been shown to regulate weight reduction as well as free fatty acid oxidation in mouse muscle and liver (2, 12). The full-length recombinant Adiponectin protein is apparently less potent at mediating these effects (2, 12). The mechanism underlying the role of Adiponectin in lipid oxidation may involve the regulation of expression or activity of proteins associated with triglyceride metabolism including CD36, acyl CoA oxidase, AMPK, and PPAR gamma (12-14). 
Although Adiponectin-regulation of glucose and lipid metabolism in humans is less clear, similar mechanisms may also be in place (15). A negative correlation between obesity and circulating Adiponectin has been well established (6, 16, 17), and Adiponectin levels increase concomitantly with weight loss (18). Decreased Adiponectin levels are associated with insulin resistance and hyperinsulinemia, and patients with type-2 diabetes are reported to exhibit decreased circulating Adiponectin (19, 20). Thiazolidinediones, a class of insulin-sensitizing, anti-diabetic drugs, elevate Adiponectin in insulin-resistant patients (21). In addition, high Adiponectin levels are associated with a reduced risk of type-2 diabetes (22). Using magnetic resonance spectroscopy it has been demonstrated that intracellular lipid content in human muscle negatively correlates with Adiponectin levels, potentially due to Adiponectin-induced fatty acid oxidation (15). 
Adiponectin may also play anti-atherogenic and anti-inflammatory roles. Adiponectin plasma levels are decreased in patients with coronary artery disease (20). Furthermore, neointimal thickening of damaged arteries is exacerbated in Adiponectin-deficient mice and is inhibited by exogenous Adiponectin (23). Adiponectin inhibits endothelial cell expression of adhesion molecules in vitro, suppressing the attachment of monocytes (24). In addition, Adiponectin negatively regulates myelomonocytic progenitor cell growth and TNF-alpha production in macrophages (25, 26).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Adiponectin/Acrp30 (DRP300)(117)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: ELISA Capture.


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ELISA Capture
(1)
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Human
(116)
Mouse
(1)
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Showing Publications 1 - 10 of 117. Show All 117 Publications.
Publications using DRP300 Applications Species
K Oluwagbemi, A Anesi, M Ulaszewska, G Clarke, U Alexy, M Schmid, M Roden, C Herder, F Mattivi, U Nöthlings Longitudinal relationship of amino acids and indole metabolites with long-term body mass index and cardiometabolic risk markers in young individuals Sci Rep, 2020;10(1):6399. 2020 [PMID: 32286421] (Human) Human
J Gorwood, T Ejlalmanes, C Bourgeois, M Mantecon, C Rose, M Atlan, D Desjardins, R Le Grand, B Fève, O Lambotte, J Capeau, V Béréziat, C Lagathu SIV Infection and the HIV Proteins Tat and Nef Induce Senescence in Adipose Tissue and Human Adipose Stem Cells, Resulting in Adipocyte Dysfunction Cells, 2020;9(4):. 2020 [PMID: 32244726] (Human) Human
S Lucht, F Hennig, S Moebus, S Ohlwein, C Herder, B Kowall, KH Jöckel, B Hoffmann All-source and source-specific air pollution and 10-year diabetes Incidence: Total effect and mediation analyses in the Heinz Nixdorf recall study Environ Int, 2020;136(0):105493. 2020 [PMID: 31991234] (Human) Human
AE O'Mara, JW Johnson, JD Linderman, RJ Brychta, S McGehee, LA Fletcher, YA Fink, D Kapuria, TM Cassimatis, N Kelsey, C Cero, Z Abdul-Sate, F Piccinini, AS Baskin, BP Leitner, H Cai, CM Millo, W Dieckmann, M Walter, NB Javitt, Y Rotman, PJ Walter, M Ader, RN Bergman, P Herscovitc, KY Chen, AM Cypess Chronic mirabegron treatment increases human brown fat, HDL cholesterol, and insulin sensitivity J. Clin. Invest., 2020;0(0):. 2020 [PMID: 31961826] (Human) Human
S Sindhu, R Thomas, S Kochumon, A Wilson, M Abu-Farha, A Bennakhi, F Al-Mulla, R Ahmad Increased Adipose Tissue Expression of Interferon Regulatory Factor (IRF)-5 in Obesity: Association with Metabolic Inflammation Cells, 2019;8(11):. 2019 [PMID: 31718015] (Human) Human
T Sawaguchi, T Nakajima, A Haruyama, T Hasegawa, I Shibasaki, T Nakajima, H Kaneda, T Arikawa, S Obi, M Sakuma, H Ogawa, Y Takei, S Toyoda, F Nakamura, S Abe, H Fukuda, T Inoue Association of serum leptin and adiponectin concentrations with echocardiographic parameters and pathophysiological states in patients with cardiovascular disease receiving cardiovascular surgery PLoS ONE, 2019;14(11):e0225008. 2019 [PMID: 31703113] (Human) Human
MJ Valente, S Rocha, S Coimbra, C Catarino, P Rocha-Pere, E Bronze-da-, JG Oliveira, J Madureira, JC Fernandes, M do Sameiro, V Miranda, L Belo, A Santos-Sil Long Pentraxin 3 as a Broader Biomarker for Multiple Risk Factors in End-Stage Renal Disease: Association with All-Cause Mortality Mediators Inflamm., 2019;2019(0):3295725. 2019 [PMID: 31316299] (Human) Human
AMB Menezes, PD Oliveira, FC Wehrmeiste, MCF Assunção, IO Oliveira, L Tovo-Rodri, GD Ferreira, H Gonçalves Association of modifiable risk factors and IL-6, CRP, and adiponectin: Findings from the 1993 Birth Cohort, Southern Brazil PLoS ONE, 2019;14(5):e0216202. 2019 [PMID: 31071114] (Human) Human
HJ Hwang, Y Liu, HS Kim, H Lee, Y Lim, H Park Daily walnut intake improves metabolic syndrome status and increases circulating adiponectin levels: randomized controlled crossover trial Nutr Res Pract, 2019;13(2):105-114. 2019 [PMID: 30984354] (Human) Human
AK Ziegler, SM Jensen, P Schjerling, AL Mackey, JL Andersen, M Kjaer The effect of resistance exercise upon age-related systemic and local skeletal muscle inflammation Exp. Gerontol., 2019;121(0):19-32. 2019 [PMID: 30905721] (Human) Human
Show All 117 Publications.

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Product General Protocols

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FAQs for Adiponectin/Acrp30 (DRP300). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP DRP300
HRP DRP300
HRP DRP300

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Bioinformatics

Gene Symbol ADIPOQ