LEDGF Antibody - BSA Free

Immunohistochemistry-Paraffin: LEDGF Antibody [NB110-40560] - Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (NB110-40560). Secondary: HRP-conjugated goat anti-rabbit IgG.

Immunoprecipitation: LEDGF Antibody [NB110-40560] - Detection of human LEDGF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LEDGF antibody NB110-40560 used for IP at 3 ug per reaction. LEDGF was also immunoprecipitated by rabbit anti-LEDGF antibody NB110-40561. For blotting immunoprecipitated LEDGF, NB110-40560 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

• Reactivity: Hu, Mu, Fe
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

LEDGF Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LEDGF Antibody - BSA Free (NB110-40560) is a polyclonal antibody validated for use in IHC and IP. Anti-LEDGF Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 225 and 275 of human Lens Epithelium Derived Growth Factor/Transcriptional Coactivator p75 using the numbering given in entry NP_150091.2 (GeneID 11168).
• Predicted Species: Feline (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSIP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500- 1:2000
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: For western blot, use rabbit anti-LEDGF Antibody NB110-40561. Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Reactivity Notes

Cat (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LEDGF Antibody - BSA Free

• CLL-associated antigen KW-7
• Dense fine speckles 70 kDa protein
• DFS 70
• DFS70
• LEDGF
• LEDGFDFS 70
• Lens epithelium-derived growth factor
• MGC74712
• p52
• p75
• PAIP
• PC4 and SFRS1 interacting protein 1
• PC4 and SFRS1 interacting protein 2
• PC4 and SFRS1-interacting protein
• PSIP1
• PSIP2
• transcriptional coactivator p52/p75
• Transcriptional coactivator p75/p52

Background

The human p75 protein is a non TAF transcription coactivator that mediates activator dependent transcription by RNA polymerase II in vitro through most tested activators. Although p75 and p52 are derived from alternatively splicing of a single gene and share most coding sequence, they reveal different function in several aspects. In addition to functioning as a transcription coactivator, p75 has been reported to be involved in regulating growth of epithelial cells, pathogenesis of atopic dermatitis and activity of HIV1 integrase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LEDGF Antibody (NB110-40560)(5)

Publications using NB110-40560

• Basu A, Rojas H, Banerjee H et al. Expression of the stress response oncoprotein LEDGF/p75 in human cancer: a study of 21 tumor types. PLoS One. 2012-01-01 [PMID: 22276150]
  
  Details:
  Tissue Microarrays cited for analysis of LEDGF/p75 protein expression (Tables 1, 3, 4; Figs 3, 4): IMH-303: Prostate cancer IMH-326: Common cancers (A) IMH-327: Common cancers (B) IMH-328: Common cancers (C) IMH-336: Matched normal adjacent of IMH-326 IMH-337: Matched normal adjacent of IMH-327 IMH-338: Matched normal adjacent of IMH-328
• Hendrix J, Gijsbers R, De Rijck J et al. The transcriptional co-activator LEDGF/p75 displays a dynamic scan-and-lock mechanism for chromatin tethering. Nucleic Acids Res 2011-03-01 [PMID: 20974633]
• Bartholomeeusen K, Christ F, Hendrix J et al. Lens epithelium-derived growth factor/p75 interacts with the transposase-derived DDE domain of PogZ. J Biol Chem 2009-04-01 [PMID: 19244240]
• Shun MC, Botbol Y, Li X et al. Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity. J Virol 2008-12-01 [PMID: 18799576]
• Shun MC, Raghavendra NK, Vandegraaff N et al. LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration. Genes Dev. 2007-07-15 [PMID: 17639082]

Bioinformatics

• Gene Symbol: PSIP1
• Entrez:
  • 11168(Human)
• Uniprot:
  • O75475()