Fascin Antibody (833223) [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Fascin at approximately 61 kDa (as indicated) using 10 µg/mL of Mouse ...read more
Fascin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Fascin Monoclonal Antibody (Catalog # MAB7745) at 15 µg/mL overnight at 4 °C. Before incubation with the primary ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Fascin knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, KO
Clone
833223
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price

Fascin Antibody (833223) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Fascin
Met1-Tyr493
Accession # Q16658
Marker
Dendritic Cell Marker
Specificity
Detects human Fascin in ELISAs and Western blots. Detects mouse and rat Fascin in Western blots. In direct ELISAs, no cross-reactivity with recombinant human Fascin-2 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
FSCN1
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Knockout Validated
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Control
Fascin Knockout HeLa Cell Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
MAB7745 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fascin Antibody (833223) [Unconjugated]

  • FAN1
  • FAN1,55 kDa actin-bundling protein
  • fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
  • Fascin
  • FLJ38511
  • FSCN1
  • HSN
  • p55actin bundling protein
  • singed (Drosophila)-like (sea urchin fascin homolog like)
  • singed-like (fascin homolog, sea urchin)
  • Singed-like Protein
  • SNL
  • SNLfascin

Background

Fascin (that which creates fascicles [bundles] of actin; also known as 55 kDa actin-bundling protein, p55 and singed-like protein) is an intracellular 55-58 kDa member of the fascin family of proteins. It has a restricted expression pattern, being found in oligodendrocytes, select endothelium, cerebellar stellate neurons and blood, interdigitating, and thymic medullary dendritic cells. Fascin is found associated with actin in filopodia, and serves to coordinate and stabilize actin bundle formation, both in normal cells and tumor cells. In the latter cell type, filopodia have been renamed invadopodia, and their appearance is crucial for the creation of a stable platform that coordinates local matrix degradation. Human fascin is 493 amino acids (aa) in length. It contains an N-terminal fascin-like domain (aa 139-256) that contains part of one of two actin-binding sequences (aa 136-143), followed by two additional fascin-like domains (aa 260-378 and 383-493), the latter of which contains the second actin-binding sequence (aa 386-395). There are also two acetylation sites and two utilized phosphorylation sites at Ser38 and Ser39. Phosphorylation of the latter site inhibits fascin interaction with actin. At least two isoform variants may exist. One contains a 12 aa substitution for aa 427-493, while a second shows a deletion of aa 371-426. Full-length human fascin shares 97% aa sequence identity with mouse fascin. Two additional human fascins termed retinal and testis fascin have been identified. They are products of distinct genes and share 56% and 27% aa sequence identity with the standard (p55) fascin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fascin Antibody (MAB7745) (0)

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Reviews for Fascin Antibody (MAB7745) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Rat.

Reviews using MAB7745:
Filter by Applications
WB
(1)
IF
(1)
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Rat
(2)
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Images Ratings Applications Species Date Details
Western Blot Fascin MAB7745
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reviewed by:
Anonymous
WB Rat 06/05/2015
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Summary

ApplicationWestern Blot
Sample TestedNRK whole cell lysate
SpeciesRat

Blocking

Blocking Details5% NFDM for 1 h

Primary Anitbody

Dilution Ratio1:0000, 4 degree, overnight

Secondary Antibody

Secondary DescriptionPeroxidase-conjugated AffiniPure Goat Anti-Mouse IgG Secondary Antibody

Details

Detection NotesECL using the Femto ECL ( Pierce #34095) and image on the Chemdoc. Exposure time: 6s
Immunofluorescence Fascin MAB7745
Enlarge
reviewed by:
Anonymous
IF Rat 05/22/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedNRK cells
SpeciesRat
LotLHPW0113011

Blocking

Blocking Details1% BSA for 1 h

Primary Anitbody

Dilution Ratio10 ug/mL overnight at 4 °C

Secondary Antibody

Secondary DescriptionGoat Anti-Mouse IgG FITC conjugated secondary antibody
Secondary Manufacturer Cat#Sigma, #091M6281
Secondary Concentration1:200 dilution

Details

Detection Notes40*, exposure time 3s.
Wash DescriptionPBS twice for 10min

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Fascin Antibody (MAB7745) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for Fascin Antibody (MAB7745)

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
06/05/2015
Application: WB
Species: Rat

Anonymous
05/22/2015
Application: IF
Species: Rat

Bioinformatics

Gene Symbol FSCN1
Uniprot