MPP1 Antibody (1E11-1G11)

Western Blot: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of monoclonal antibody to MPP1 on HeLa cell . Antibody concentration 10 ug/ml.

Western Blot: MPP1 Antibody (1E11-1G11) [H00004354-M01] - MPP1 monoclonal antibody (M01), clone 1E11-1G11. Analysis of MPP1 expression in human placenta.

Immunoprecipitation: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of MPP1 transfected lysate using anti-MPP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MPP1 monoclonal antibody.

Sandwich ELISA: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Detection limit for recombinant GST tagged MPP1 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 1E11-1G11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MPP1 Antibody (1E11-1G11) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MPP1 (AAH02392, 1 a.a. ~ 466 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTLKASEGESGGSMHTALSDLYLEHLLQKRSRPEAVSHPLNTVTEDMYTNGSPAPGSPAQVKGQEVRKVRLIQFEKVTEEPMGITLKLNEKQSCTVARILHGGMIHRQGSLHVGDEILEINGTNVTNHSVDQLQKAMKETKGMISLKVIPNQQSRLPALQMFMRAQFDYDPKKDNLIPCKEAGLKFATGDIIQIINKDDSNWWQGRVEGSSKESAGLIPSPELQEWRVASMAQSAPSEAPSCSPFGKKKKYKDKYLAKHSSIFDQLDVVSYEEVVRLPAFKRKTLVLIGASGVGRSHIKNALLSQNPEKFVYPVPYTTRPPRKSEEDGKEYHFISTEEMTRNISANEFLEFGSYQGNMFGTKFETVHQIHKQNKIAILDIEPQTLKIVRTAELSPFIVFIAPTDQGTQTEALQQLQKDSEAIRSQYAHYFDLSLVNNGVDETLKKLQEAFDQACSSPQWVPVSWVY
• Specificity: MPP1 - membrane protein, palmitoylated 1, 55kDa
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MPP1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MPP1 Antibody (1E11-1G11)

• 55 kDa erythrocyte membrane protein
• AAG12
• EC 2.7.11
• Membrane protein, palmitoylated 1
• membrane protein, palmitoylated 1, 55kDa
• MRG1
• palmitoylated erythrocyte membrane protein
• PEMPpalmitoylated 1 (55kD)

Background

Palmitoylated membrane protein 1 is the prototype of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 1 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MPP1 Antibody (H00004354-M01)(2)

Publications using H00004354-M01

• Agnieszka B, Karolina W, Tomasz T et al. MPP1 Determines the Mobility of Flotillins and Controls the Confinement of Raft-Associated Molecules. Cells. 2022-01-18 [PMID: 35159121]
• Biernatowska A, Olszewska P, Tabaczar S, and Sikorski AF The microdomain-organizing protein MPP1 is required for insulin-stimulated activation of H-Ras. Oncotarget. 2018-04-06 [PMID: 29719614]

Bioinformatics

• Gene Symbol: MPP1
• Entrez:
  • 4354(Human)
• OMIM:
  • 305360(Human)
• Uniprot:
  • AAH02392(Human)