Home » MPP1 » MPP1 Antibodies » MPP1 Antibody (1E11-1G11)

MPP1 Antibody (1E11-1G11)

 Submit a Review

Images

 
(
Enlarge)
Western Blot: MPP1 Antibody (1E11-1G11) [H00004354-M01] - MPP1 monoclonal antibody (M01), clone 1E11-1G11. Analysis of MPP1 expression in human placenta.
Western Blot: MPP1 Antibody (1E11-1G11) [H00004354-M01] - MPP1 monoclonal antibody (M01), clone 1E11-1G11. Analysis of MPP1 expression in human placenta.
Immunocytochemistry/ Immunofluorescence: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of monoclonal antibody to MPP1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 ...read more
Immunoprecipitation: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of MPP1 transfected lysate using anti-MPP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MPP1 monoclonal antibody.
Sandwich ELISA: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Detection limit for recombinant GST tagged MPP1 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
1E11-1G11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

MPP1 Antibody (1E11-1G11) Summary

Immunogen
MPP1 (AAH02392, 1 a.a. - 466 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTLKASEGESGGSMHTALSDLYLEHLLQKRSRPEAVSHPLNTVTEDMYTNGSPAPGSPAQVKGQEVRKVRLIQFEKVTEEPMGITLKLNEKQSCTVARILHGGMIHRQGSLHVGDEILEINGTNVTNHSVDQLQKAMKETKGMISLKVIPNQQSRLPALQMFMRAQFDYDPKKDNLIPCKEAGLKFATGDIIQIINKDDSNWWQGRVEGSSKESAGLIPSPELQEWRVASMAQSAPSEAPSCSPFGKKKKYKDKYLAKHSSIFDQLDVVSYEEVVRLPAFKRKTLVLIGASGVGRSHIKNALLSQNPEKFVYPVPYTTRPPRKSEEDGKEYHFISTEEMTRNISANEFLEFGSYQGNMFGTKFETVHQIHKQNKIAILDIEPQTLKIVRTAELSPFIVFIAPTDQGTQTEALQQLQKDSEAIRSQYAHYFDLSLVNNGVDETLKKLQEAFDQACSSPQWVPVSWVY
Specificity
MPP1 - membrane protein, palmitoylated 1, 55kDa
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MPP1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MPP1 Antibody (1E11-1G11)

  • 55 kDa erythrocyte membrane protein
  • AAG12
  • EC 2.7.11
  • Membrane protein, palmitoylated 1
  • membrane protein, palmitoylated 1, 55kDa
  • MRG1
  • palmitoylated erythrocyte membrane protein
  • PEMPpalmitoylated 1 (55kD)

Background

Palmitoylated membrane protein 1 is the prototype of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 1 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

TNF-alpha
[Unconjugated]
210-TA
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
(20 Reviews)
(516 Publications)
Species: Hu
IL-2 [Unconjugated]
202-IL
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
(11 Reviews)
(239 Publications)
Species: Hu
IL-2 R beta
Antibody (27302)
MAB224
IL‑2 R beta was detected in immersion fixed frozen sections of human lymph node using 15 µg/mL Mouse Anti-Human IL‑2 R beta Monoclonal Antibody (Catalog # MAB224) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑2 R beta Monoclonal Antibody (Catalog # MAB224). The ND50 is typically 0.05‑0.15 µg/mL.
(5 Publications)
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
CDP/CUTL1 Antibody
NBP2-13883
Western Blot: CDP/CUTL1 Antibody [NBP2-13883] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted)
Immunocytochemistry/Immunofluorescence: CDP/CUTL1 Antibody [NBP2-13883] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CDP/CUTL1 Antibody [NBP2-13883] - Staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.
(1 Review)
(1 Publication)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
LEDGF Antibody
NB110-40561
Western Blot: LEDGF Antibody [NB110-40561] - Detection of Human LEDGF/p75 on HeLa whole cell lysate using NB110-40561. LEDGF/p75 was also immunoprecipitated by another rabbit anti-LEDGF/p75 antibody.
(7 Publications)
Species: Hu
Applications: WB, IP
KHSRP Antibody
NBP1-18910
Western Blot: KHSRP Antibody [NBP1-18910] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antiKSRP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: KHSRP Antibody [NBP1-18910] - FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-KSRP used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Western Blot: KHSRP Antibody [NBP1-18910] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. KSRP was also immunoprecipitated by rabbit anti-KSRP antibody NBP1-18911.
(1 Review)
(5 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
HCLS1 Antibody
AF5789
Western blot shows lysates of L1.2 mouse pro‑B cell line, Nb2‑11 rat lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat HCLS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5789) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HCLS1 at approximately 75 to 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu, Mu, Rt
Applications: WB
Siglec-7/CD328
Antibody [Unconjugate
AF1138
Recombinant Human Siglec‑7 Fc Chimera (Catalog # 1138-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec‑7 Fc Chimera (1.5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Siglec‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1138). The ND50 is typically 0.3-0.9 µg/mL.
(3 Publications)
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
CTLA-4 Antibody
[Unconjugated]
AF-386-PB
Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human CTLA-4. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CTLA‑4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NS0 mouse myeloma cell line co-transfected with human CTLA-4 and eGFP was stained with either (A) Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
CTLA‑4 was detected in immersion fixed human peripheral blood mononuclear cells treated with treated with PMA and calcium ionomycin using Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
(2 Reviews)
(2 Publications)
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
HLA DQA1 Antibody
NBP1-84550
Western Blot: HLA DQA1 Antibody [NBP1-84550] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line Daudi.
Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human lung shows high expression.
Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
Species: Hu
Applications: WB, IHC, IHC-P
NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
(1 Review)
(13 Publications)
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
IL-4 [Unconjugated]
204-IL
1 μg/lane of Recombinant Human IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human IL-4 (Catalog # 204-IL) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.05-02 ng/mL.
(8 Reviews)
(286 Publications)
Species: Hu
TPPP/p25 Antibody
NBP1-49833
Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) Analysis of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, PEP-ELISA
IL-1 beta/IL-1F2
[Unconjugated]
201-LB
1 μg/lane of Recombinant Human IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is <12 pg/mL.
(7 Reviews)
(329 Publications)
Species: Hu

Calcium-binding-protei
NBP1-33736
Western Blot: Calcium-binding-protein-P22 Antibody [NBP1-33736] - A. 50 ug mouse liver lysate/extract 12 % SDS-PAGE
Immunocytochemistry/Immunofluorescence: Calcium-binding-protein-P22 Antibody [NBP1-33736] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcium binding protein P22 protein stained by Calcium binding protein P22 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Calcium-binding-protein-P22 Antibody [NBP1-33736] - Paraffin-embedded mouse lung. Calcium binding protein P22 antibody dilution: 1:500.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MPP1 Antibody
(1E11-1G11)
H00004354-M01
MPP1 Antibody (1E11-1G11)
Species: Hu
Applications: WB, ELISA, ICC/IF, IP

Publications for MPP1 Antibody (H00004354-M01) (0)

There are no publications for MPP1 Antibody (H00004354-M01).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MPP1 Antibody (H00004354-M01) (0)

There are no reviews for MPP1 Antibody (H00004354-M01). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MPP1 Antibody (H00004354-M01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for MPP1 Antibody (H00004354-M01)

Discover related pathways, diseases and genes to MPP1 Antibody (H00004354-M01). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MPP1 Antibody (H00004354-M01)

Discover more about diseases related to MPP1 Antibody (H00004354-M01).
 

Pathways for MPP1 Antibody (H00004354-M01)

View related products by pathway.

PTMs for MPP1 Antibody (H00004354-M01)

Learn more about PTMs related to MPP1 Antibody (H00004354-M01).
 

Research Areas for MPP1 Antibody (H00004354-M01)

Find related products by research area.

Blogs on MPP1

There are no specific blogs for MPP1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MPP1 Antibody (1E11-1G11) and receive a gift card or discount.


Bioinformatics

Gene Symbol MPP1
Entrez
OMIM
Uniprot