ERG Antibody (SP06-04)

Western Blot: ERG Antibody (SP06-04) [NBP2-67375] - Analysis of ERG on Jurkat cell lysates using anti-ERG antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: ERG Antibody (SP06-04) [NBP2-67375] - Staining ERG in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ERG Antibody (SP06-04) [NBP2-67375] - Analysis of paraffin-embedded mouse brain tissue using anti-ERG antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/Immunofluorescence: ERG Antibody (SP06-04) [NBP2-67375] - Staining ERG in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ERG Antibody (SP06-04) [NBP2-67375] - Analysis of paraffin-embedded human colon cancer tissue using anti-ERG antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ERG Antibody (SP06-04) [NBP2-67375] - Analysis of paraffin-embedded mouse brain tissue using anti-ERG antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ERG Antibody (SP06-04) [NBP2-67375] - Analysis of paraffin-embedded mouse heart tissue using anti-ERG antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ERG Antibody (SP06-04) [NBP2-67375] - Analysis of paraffin-embedded human colon carcinoma tissue using anti-ERG antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: ERG Antibody (SP06-04) [NBP2-67375] - Analysis of paraffin-embedded human spleen tissue using anti-ERG antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ChIP, ICC/IF, IHC
• Clone: SP06-04
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ERG Antibody (SP06-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ERG aa 430-479 / 479. (SwissProt: P11308 Human; SwissProt: P81270 Mouse; Unigene: 47987 Rat)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ERG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ERG Antibody (SP06-04)

• transcriptional regulator ERG
• 10ets-related
• Erg
• Erg-3
• p55
• TMPRSS2-ERG prostate cancer specific
• transcriptional regulator ERG
• Transforming protein ERG
• v-ets erythroblastosis virus E26 oncogene homolog (avian)
• v-ets erythroblastosis virus E26 oncogene like (avian)
• v-ets erythroblastosis virus E26 oncogene like

Background

"ERG is a transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for ERG Antibody (NBP2-67375). (Showing 1 - 1 of 1 FAQ).

1. I am looking for an antibody that can IP Erg protein and doesn't cross react with Fli protein.
   • I have spoken to the lab and they have informed me that actually that the antibody (catalog #NBP1-95857) is confirmed not to cross react with Fli1 protein so it should be a viable choice for you in your experiments on Erg.

Bioinformatics

• Gene Symbol: ERG