| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3468-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3468-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87885-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22622-50ul | |||
![Immunocytochemistry/Immunofluorescence: LEDGF Antibody [NBP1-87885] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87885-img0008.jpg "Immunocytochemistry/Immunofluorescence: LEDGF Antibody [NBP1-87885] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LEDGF Antibody [NBP1-87885] - Staining in human cerebral cortex and liver tissues using anti-PSIP1 antibody. Corresponding PSIP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunohistochemistry-Paraffin-NBP1-87885-img0007.jpg)
![Immunohistochemistry-Paraffin: LEDGF Antibody [NBP1-87885] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunohistochemistry-Paraffin-NBP1-87885-img0005.jpg)
![Immunohistochemistry-Paraffin: LEDGF Antibody [NBP1-87885] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunohistochemistry-Paraffin-NBP1-87885-img0006.jpg)
![Immunohistochemistry-Paraffin: LEDGF Antibody [NB110-40560] - Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (NB110-40560). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunohistochemistry-Paraffin-NB110-40560-img0005.jpg "Immunohistochemistry-Paraffin: LEDGF Antibody [NB110-40560] - Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (NB110-40560). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: LEDGF Antibody [NB110-40560] - Detection of human LEDGF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LEDGF antibody NB110-40560 used for IP at 3 ug per reaction. LEDGF was also immunoprecipitated by rabbit anti-LEDGF antibody NB110-40561. For blotting immunoprecipitated LEDGF, NB110-40560 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunoprecipitation-NB110-40560-img0006.jpg)
![Western Blot: LEDGF Antibody [NB110-40561] - Detection of Human LEDGF/p75 on HeLa whole cell lysate using NB110-40561. LEDGF/p75 was also immunoprecipitated by another rabbit anti-LEDGF/p75 antibody.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Western-Blot-NB110-40561-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: LEDGF Antibody [NB110-40561] - Detection of Human LEDGF/p75 on HeLa whole cell lysate using NB110-40561. LEDGF/p75 was also immunoprecipitated by another rabbit anti-LEDGF/p75 antibody.")
![Western Blot: LEDGF Antibody (PSH02-55) [NBP3-32860] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: THP-1 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32860) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-288202412442993.jpg "Western Blot: LEDGF Antibody (PSH02-55) [NBP3-32860] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: THP-1 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32860) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32860) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-298202414333682.jpg)
![Immunohistochemistry: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling LEDGF with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32860, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-29820241420980.jpg)
![Immunocytochemistry/ Immunofluorescence: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunocytochemistry analysis of SH-SY5Y cells labeling LEDGF with Mouse anti-LEDGF antibody (NBP3-32860) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-LEDGF antibody (NBP3-32860) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-29202410234271.jpg)
![Western Blot: LEDGF Antibody (3H1) [H00011168-M01] - PSIP1 monoclonal antibody (M01), clone 3H1. Analysis of PSIP1 expression in human liver.](http://images.novusbio.com/fullsize/LEDGF-Antibody-3H1-Western-Blot-H00011168-M01-img0007.jpg "Western Blot: LEDGF Antibody (3H1) [H00011168-M01] - PSIP1 monoclonal antibody (M01), clone 3H1. Analysis of PSIP1 expression in human liver.")
![Immunohistochemistry-Paraffin: LEDGF Antibody (3H1) [H00011168-M01] - Analysis of monoclonal antibody to PSIP1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/LEDGF-Antibody-3H1-Immunohistochemistry-Paraffin-H00011168-M01-img0005.jpg)
![Sandwich ELISA: LEDGF Antibody (3H1) [H00011168-M01] - Detection limit for recombinant GST tagged PSIP1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LEDGF-Antibody-3H1-Sandwich-ELISA-H00011168-M01-img0006.jpg)
![Western Blot: LEDGF Antibody (1C4) [H00011168-M02] - PSIP1 monoclonal antibody (M02), clone 1C4. Analysis of PSIP1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/LEDGF-Antibody-1C4-Western-Blot-H00011168-M02-img0006.jpg "Western Blot: LEDGF Antibody (1C4) [H00011168-M02] - PSIP1 monoclonal antibody (M02), clone 1C4. Analysis of PSIP1 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: LEDGF Antibody (1C4) [H00011168-M02] - Analysis of monoclonal antibody to PSIP1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/LEDGF-Antibody-1C4-Immunocytochemistry-Immunofluorescence-H00011168-M02-img0005.jpg)
![Western Blot: LEDGF Antibody (1C4) [H00011168-M02] - PSIP1 monoclonal antibody (M02), clone 1C4. Analysis of PSIP1 expression in human kidney.](http://images.novusbio.com/fullsize/LEDGF-Antibody-1C4-Western-Blot-H00011168-M02-img0007.jpg)
![Western Blot: LEDGF Antibody (JE75-02) [NBP3-32859] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32859) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: Huh7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32859) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32859_rabbit-ledgf-mab-je75-02-288202412461663.jpg "Western Blot: LEDGF Antibody (JE75-02) [NBP3-32859] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32859) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: Huh7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32859) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LEDGF Antibody (JE75-02) [NBP3-32859] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-LEDGF antibody (NBP3-32859) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32859) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32859_rabbit-ledgf-mab-je75-02-29820241420993.jpg)
![Western Blot: LEDGF Antibody (SR2191) [NBP3-22622] - Western blot analysis using NBP3-22622 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22622_rabbit-ledgf-mab-sr2191-5720231624742.jpg "Western Blot: LEDGF Antibody (SR2191) [NBP3-22622] - Western blot analysis using NBP3-22622 in HeLa cell lysate.")