LAMP-1/CD107a Antibody

Images

 
Western Blot: LAMP-1/CD107a Antibody [NB120-19294] - Analysis of 20 ug of HeLa cell lysate and 5 ul of Molecular Weight Protein Ladder per well.
Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Cathepsin D (CathD) expression and localization in primary neurons. Colocalization of transfected CathD with LAMP-1/CD107a. CathD ...read more
Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Lamp1 (red) in HeLa cells.
Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody [NB120-19294] - Cathepsin B (CathB) expression and localization in primary neurons. Colocalization of transfected CathB with LAMP-1/CD107a. CathB ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

LAMP-1/CD107a Antibody Summary

Immunogen
Synthetic peptide corresponding to residues C K(407) R S H A G Y Q T I(416) of human LAMP1.
Localization
Type I membrane protein. Lysosomal. This protein shuttles between lysosomes, endosomes, and the plasma membrane.
Marker
Late Endosome Marker
Specificity
LAMP1 - Lysosome Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LAMP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
ICC usage was reported and validated in scientific literature. Reactivity for this application may vary lot to lot.
Control
Human Brain Cerebellum Membrane Tissue Lysate (Adult Membrane Normal)
Reviewed Applications
Read 2 Reviews rated 3.5
using
NB120-19294 in the following applications:

Publications
Read Publications using
NB120-19294 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22453828 ). Rat reactivity reported in scientific literature (PMID: 22544351).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02M tris (pH 7.4) and 0.1M glycine
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LAMP-1/CD107a Antibody

  • CD107 antigen-like family member A
  • CD107a antigen
  • CD107a
  • LAMP1
  • LAMP-1
  • LAMPA
  • LGP120
  • lysosomal-associated membrane protein 1
  • lysosome-associated membrane glycoprotein 1
  • Lysosome-associated membrane protein 1

Background

Lysosomes are membrane-bound vacuoles which play a critical role in cellular metabolism. These organelles participate in endocytosis, the synthesis and assembly of acid hydrolases, act as sites for digestion of foreign materials and for specialized autolytic cellular processes. Membrane-associated glycoproteins have an important role in normal lysosomal function. Lamp1, also known as lysosome associated membrane protein 1, is a heavily glycosylated membrane protein of the lysosome. It contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LAMP-1/CD107a Antibody (NB120-19294)(24)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(18)
IHC
(1)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(7)
Mouse
(8)
Rat
(4)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB120-19294 Applications Species
Pandey K, Yu XW, Steinmetz A, Alberini CM Autophagy coupled to translation is required for long-term memory Autophagy Jun 5 2020 [PMID: 32501746] (ICC/IF, Rat) ICC/IF Rat
Saha P, Shumate JL, Caldwell JG et al. Inter-domain dynamics drive cholesterol transport by NPC1 and NPC1L1 proteins Elife May 15 2020 [PMID: 32410728] (ICC/IF, Mouse) ICC/IF Mouse
Mittal E, Skowyra ML, Uwase G et al. Mycobacterium tuberculosis Type VII Secretion System Effectors Differentially Impact the ESCRT Endomembrane Damage Response. MBio. Nov 27 2018 [PMID: 30482832] (ICC/IF, Mouse) ICC/IF Mouse
Xie W, Zheng W, Liu M et al. BRF1 ameliorates LPS-induced inflammation through autophagy crosstalking with MAPK/ERK signaling Genes Dis Sep 1 2018 [PMID: 30320187] (ICC/IF, Mouse) ICC/IF Mouse
Kevadiya BD, Woldstad C, Ottemann BM et al. Multimodal Theranostic Nanoformulations Permit Magnetic Resonance Bioimaging of Antiretroviral Drug Particle Tissue-Cell Biodistribution Theranostics 2018 Jan 01 [PMID: 29290806] (ICC/IF, Human) ICC/IF Human
Koster S, Upadhyay S, Chandra P et al. Mycobacterium tuberculosis is protected from NADPH oxidase and LC3-associated phagocytosis by the LCP protein CpsA. PNAS. 2017 Sep 05 [PMID: 28973896] (ICC/IF) ICC/IF
Kjellman M AN IN VITRO MODEL FOR LYSOSOMAL STORAGE DISEASES USING ASPARTYLGLUCOSAMINURIA PATIENT CELLS Thesis Jan 1 2020 (ICC/IF, Human) ICC/IF Human
Ahmed N, Yang P, Huang Y et al. Entosis Acts as a Novel Way within Sertoli Cells to Eliminate Spermatozoa in Seminiferous Tubule. Front Physiol. 2017 Jun 14 [PMID: 28611685] (IHC-P) IHC-P
Gouwens Lisa K, Makoni Nyasha J, Rogers Victoria A et al. Amyloid-B42 protofibrils are internalized by microglia more extensively than monomers. Brain Research 2016 [PMID: 27531183] (ICC/IF, Mouse) ICC/IF Mouse
Sidjanin DJ, Park AK, Ronchetti A et al. TBC1D20 mediates autophagy as a key regulator of autophagosome maturation. Autophagy. Aug 3 2016 [PMID: 27487390] (IHC, Mouse) IHC Mouse
Show All 24 Publications.

Reviews for LAMP-1/CD107a Antibody (NB120-19294) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB120-19294:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry LAMP-1/CD107a NB120-19294
Enlarge
reviewed by:
Scott Messenger
ICC Human 12/15/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedMDA-MB-231
SpeciesHuman
Lotsg251759
 LAMP-1/CD107a NB120-19294
Enlarge
reviewed by:
Anonymous
Mouse 12/08/2017
View

Summary

Sample TestedPrimary mouse hepatocytes
SpeciesMouse
LotRD232021

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LAMP-1/CD107a Antibody (NB120-19294) (0)

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Control Lysate(s)

Secondary Antibodies

 

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Discover related pathways, diseases and genes to LAMP-1/CD107a Antibody (NB120-19294). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

3.5
5
0
4
1
3
1
2
0
1
0

Scott Messenger
12/15/2017
Application: ICC
Species: Human

Anonymous
12/08/2017
Application:
Species: Mouse

Bioinformatics

Gene Symbol LAMP1
Entrez
Uniprot