Integrin alpha 4/CD49d Antibody (PS/2) [Biotin]

Flow Cytometry: Integrin alpha 4/CD49d Antibody (PS/2) [Biotin] [NBP1-26663] - BALB/c mouse splencoytes were staining with Rat Anti Mouse.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC
• Clone: PS/2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin

Integrin alpha 4/CD49d Antibody (PS/2) [Biotin] Summary

• Immunogen: P815 cells
• Specificity: CD49d, the alpha 4 chain (100 kDa) of the VLA-4 integrin heterodimer (Mr 150-kDa)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGA4
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: Sodium Azide
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for Integrin alpha 4/CD49d Antibody (PS/2) [Biotin]

• 269C wild type
• CD49 antigen-like family member D
• CD49d antigen
• CD49d
• CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
• IA4
• Integrin alpha 4
• integrin alpha-4 subunit
• integrin alpha-4
• Integrin alpha-IV
• integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• ITGA4
• MGC90518
• very late activation protein 4 receptor, alpha 4 subunit
• VLA-4 subunit alpha
• VLA-4

Background

VLA-4 belongs to the beta1-integrin family, which functions both as cell-matrix and cell-cell receptors. (1-3) As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1. (1, 2) In addition to CD44/pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1. (2, 3) CD49d/VLA-4 is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive. (1, 2) Antibody PS/2 also reacts with human VLA-4. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA4