Integrin alpha 4/CD49d Antibody (PS/2)

Images

 
Immunohistochemistry-Paraffin: Integrin alpha 4/CD49d Antibody (PS/2) [NBP1-26661] - FFPE human ovarian carcinoma. Antibody at 1:10. HIER antigen retrieval at pH 6. HRP/DAB detetion. Image from verified customer review.
Flow Cytometry: Integrin alpha 4/CD49d Antibody (PS/2) [NBP1-26661] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD49d-UNLB followed by Mouse Anti-Rat IgG2b-AF647.
Flow Cytometry: Integrin alpha 4/CD49d Antibody (PS/2) [NBP1-26661] - Less or equal to 2 ug/10^6 cellsCells from BALB/c bone marrow were stained with rat anti-mouse CD49d: FITC, following which lymphocytes were gated ...read more
Flow Cytometry: Integrin alpha 4/CD49d Antibody (PS/2) [NBP1-26661] - Analysis using the Biotin conjugate of NBP1-26661. Multiple staining of BALB/c splenocytes.
Flow Cytometry: Integrin alpha 4/CD49d Antibody (PS/2) [NBP1-26661] - Analysis using the FITC conjugate of NBP1-26661. Staining of less or equal to 2 ug/10^6 cellsCells from BALB/c bone marrow with rat anti-mouse ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P, IP, B/N
Clone
PS/2
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Catalog# & Conjugate Size Price

Integrin alpha 4/CD49d Antibody (PS/2) Summary

Immunogen
P815 cells
Specificity
CD49d, the alpha 4 chain (100 kDa) of the VLA-4 integrin heterodimer (Mr 150-kDa)
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
ITGA4
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10 - 1:500
  • Block/Neutralize
Reviewed Applications
Read 1 Review rated 4
using
NBP1-26661 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Integrin alpha 4/CD49d Antibody (PS/2)

  • 269C wild type
  • CD49 antigen-like family member D
  • CD49d antigen
  • CD49d
  • CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
  • IA4
  • Integrin alpha 4
  • integrin alpha-4 subunit
  • integrin alpha-4
  • Integrin alpha-IV
  • integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
  • ITGA4
  • MGC90518
  • very late activation protein 4 receptor, alpha 4 subunit
  • VLA-4 subunit alpha
  • VLA-4

Background

VLA-4 belongs to the beta1-integrin family, which functions both as cell-matrix and cell-cell receptors. (1-3) As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1. (1, 2) In addition to CD44/pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1. (2, 3) CD49d/VLA-4 is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive. (1, 2) Antibody PS/2 also reacts with human VLA-4. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin alpha 4/CD49d Antibody (NBP1-26661) (0)

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Review for Integrin alpha 4/CD49d Antibody (NBP1-26661) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-26661:
Filter by Applications
IHC-P
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Integrin alpha 4/CD49d NBP1-26661
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reviewed by:
Haopei Zhang
IHC-P Human 01/04/2019
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman ovarian carcinoma
SpeciesHuman
LotK2416-N398

Comments

CommentsDilution: 1:10
Antigen Retrieval: HIER in pressure cooker for 3 minutes with sodium citrate buffer (pH=6)
Incubated at 4℃ overnight (16 hours)
HRP/DAB detection system

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FAQs for Integrin alpha 4/CD49d Antibody (NBP1-26661) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin NBP1-26663
FITC NBP1-26662

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Haopei Zhang
01/04/2019
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol ITGA4
Entrez