Integrin alpha 4/CD49d Antibody (PS/2) - Azide and BSA Free

Immunohistochemistry-Paraffin: Integrin alpha 4/CD49d Antibody (PS/2) [NBP1-26661] - FFPE human ovarian carcinoma. Antibody at 1:10. HIER antigen retrieval at pH 6. HRP/DAB detetion. IHC-P image submitted by a verified customer review.

Flow Cytometry: Integrin alpha 4/CD49d Antibody (PS/2) [NBP1-26661] - BALB/c mouse splenocytes were stained with Rat Anti-Mouse CD49d-UNLB followed by Mouse Anti-Rat IgG2b-AF647.

• Reactivity: Hu, Mu
• Applications: Flow, IHC
• Clone: PS/2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

Integrin alpha 4/CD49d Antibody (PS/2) - Azide and BSA Free Summary

• Immunogen: P815 cells
• Specificity: CD49d, the alpha 4 chain (100 kDa) of the VLA-4 integrin heterodimer (Mr 150-kDa)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGA4
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin Validated from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Integrin alpha 4/CD49d Antibody (PS/2) - Azide and BSA Free

• 269C wild type
• CD49 antigen-like family member D
• CD49d antigen
• CD49d
• CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
• IA4
• Integrin alpha 4
• integrin alpha-4 subunit
• integrin alpha-4
• Integrin alpha-IV
• integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• ITGA4
• MGC90518
• very late activation protein 4 receptor, alpha 4 subunit
• VLA-4 subunit alpha
• VLA-4

Background

VLA-4 belongs to the beta1-integrin family, which functions both as cell-matrix and cell-cell receptors. (1-3) As a matrix receptor VLA-4 binds to an alternatively spliced domain of fibronectin, while as a cellular receptor it associates with its counter-receptor ligand VCAM-1. (1, 2) In addition to CD44/pgp-1, VLA-4 has been shown to be important for lymphopoiesis, and is also believed to be important for extravasation of lymphocytes into sites of inflammation as a second signal in addition to LFA/ICAM-1. (2, 3) CD49d/VLA-4 is expressed in low amounts on resting lymphocytes and monocytes, is absent or minimally detectable on stromal cells, whereas virtually all lymphoid and myeloid cells in bone marrow and in long-term culture are positive. (1, 2) Antibody PS/2 also reacts with human VLA-4. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 4/CD49d Antibody (NBP1-26661)(1)

Publication using NBP1-26661

• Grilo GA, Cakir SN, Shaver PR et al. Collagen matricryptin promotes cardiac function by mediating scar formation Life sciences 2023-03-22 [PMID: 36963720] (B/N, Mouse)
  
  Details:
  Used as a blocking antibody at 15ug/ml in a migration assay

Review for Integrin alpha 4/CD49d Antibody (NBP1-26661) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Haopei Zhang IHC-P Human 01/04/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human ovarian carcinoma
• Species: Human
• Lot: K2416-N398

Comments

• Comments: Dilution: 1:10
  Antigen Retrieval: HIER in pressure cooker for 3 minutes with sodium citrate buffer (pH=6)
  Incubated at 4℃ overnight (16 hours)
  HRP/DAB detection system

Bioinformatics

• Gene Symbol: ITGA4
• Entrez:
  • 3676(Human)