Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric

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Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volociximab). PBMC's (A) and U93 cells (B) were stained with ...read more
Immunofluorescence: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, B/N, CyTOF-ready, ICC/IF
Clone
M200 (Volociximab)
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Heterodimeric alpha 5 Beta 1 Fc fusion protein
Specificity
The antibody binds to human (alpha)5(beta)1 integrin with a Kd of 0.367 nM (determined by Biacore), an EC50 of 0.2 nM and an IC50 of 2.3 nM.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
ITGA5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Block/Neutralize
  • CyTOF-ready
  • Immunofluorescence 1:10 - 1:500Blocking/Neutralizing 50 - 200 molar excess
Application Notes
This antibody is Cytof ready. NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques. Use in ICC/IF was reported in scientific literature (PMID: 30611716). The antibody binds specifically to Integrin alpha 5 beta 1, which is part of the superfamily of glycoprotein transmembrane receptors for ligands such as fibronectin, vitronectin, laminins and collagens. The Integrin alpha 5 beta 1 receptor plays a key role in cellular processes such as inflammation, cell proliferation, angiogenesis and tumour metastasis. Comprising the variable domain of the original mouse antibody, a chimeric mouse:human IgG4 version of this antibody has been tested in vivo: In cynomolgus monkeys with laser-induced choroidal neovascularization (CNV), in rabbit models and in human volunteers. The antibody reached Phase II clinical trials with PDL Biopharma and Biogen-Idec. Use in WB reported in secitific publication PMID: 32449990
Publications
Read Publications using
NBP2-52680 in the following applications:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Buffer
PBS
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric

  • biosimilar antibody
  • biosimilars
  • CD49 antigen-like family member E
  • CD49e
  • Fibronectin receptor subunit alpha
  • fibronectin receptor, alpha polypeptide
  • fibronectin receptor, alpha subunit
  • FNRA
  • Integrin alpha 5 beta 1
  • integrin alpha-5
  • Integrin alpha-F
  • integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
  • ITGA5/SLC9B1 fusion
  • very late activation protein 5, alpha subunit
  • VLA-5
  • VLA5A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Integrin alpha 5 beta 1 Antibody (NBP2-52680)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Integrin alpha 5 beta 1 Antibody (NBP2-52680) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-52680AF350
Alexa Fluor 405 NBP2-52680AF405
Alexa Fluor 488 NBP2-52680AF488
Alexa Fluor 532 NBP2-52680AF532
Alexa Fluor 594 NBP2-52680AF594
Alexa Fluor 647 NBP2-52680AF647
Alexa Fluor 700 NBP2-52680AF700
Alexa Fluor 750 NBP2-52680AF750
Allophycocyanin NBP2-52680APC
Biotin NBP2-52680B
DyLight 350 NBP2-52680UV
DyLight 405 NBP2-52680V
DyLight 488 NBP2-52680G
DyLight 550 NBP2-52680R
DyLight 650 NBP2-52680C
DyLight 680 NBP2-52680FR
DyLight 755 NBP2-52680IR
FITC NBP2-52680F
HRP NBP2-52680H
Janelia Fluor 549 NBP2-52680JF549
Janelia Fluor 646 NBP2-52680JF646
PE NBP2-52680PE
PerCP NBP2-52680PCP

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Bioinformatics

Gene Symbol ITGA5