Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric

Flow Cytometry: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volociximab). PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) (NBP2-52680, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunofluorescence: Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric [NBP2-52680] - Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 (NBP2-52680) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52680, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, B/N, CyTOF-ready, ICC/IF
• Clone: M200 (Volociximab)
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Heterodimeric alpha 5 Beta 1 Fc fusion protein
• Specificity: The antibody binds to human (alpha)5(beta)1 integrin with a Kd of 0.367 nM (determined by Biacore), an EC50 of 0.2 nM and an IC50 of 2.3 nM.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ITGA5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Block/Neutralize
  • CyTOF-ready
  • Immunofluorescence 1:10 - 1:500Blocking/Neutralizing 50 - 200 molar excess
• Application Notes: This antibody is Cytof ready. NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques. Use in ICC/IF was reported in scientific literature (PMID: 30611716). The antibody binds specifically to Integrin alpha 5 beta 1, which is part of the superfamily of glycoprotein transmembrane receptors for ligands such as fibronectin, vitronectin, laminins and collagens. The Integrin alpha 5 beta 1 receptor plays a key role in cellular processes such as inflammation, cell proliferation, angiogenesis and tumour metastasis. Comprising the variable domain of the original mouse antibody, a chimeric mouse:human IgG4 version of this antibody has been tested in vivo: In cynomolgus monkeys with laser-induced choroidal neovascularization (CNV), in rabbit models and in human volunteers. The antibody reached Phase II clinical trials with PDL Biopharma and Biogen-Idec. Use in WB reported in secitific publication PMID: 32449990

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin alpha 5 beta 1 Antibody (M200 (Volociximab)) - Chimeric

• biosimilar antibody
• biosimilars
• CD49 antigen-like family member E
• CD49e
• Fibronectin receptor subunit alpha
• fibronectin receptor, alpha polypeptide
• fibronectin receptor, alpha subunit
• FNRA
• Integrin alpha 5 beta 1
• integrin alpha-5
• Integrin alpha-F
• integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• ITGA5/SLC9B1 fusion
• very late activation protein 5, alpha subunit
• VLA-5
• VLA5A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 5 beta 1 Antibody (NBP2-52680)(4)

Publications using NBP2-52680

• Moller N, Ziesemer S et al. S. aureus alpha-toxin monomer binding and heptamer formation in host cell membranes - Do they determine sensitivity of airway epithelial cells toward the toxin? PLoS One May 30 2020 [PMID: 32470006] (Flow, Human)
• Smith PC, Metz C, de la PeNa A et al. Galectin-8 mediates fibrogenesis induced by cyclosporine in human gingival fibroblasts J. Periodont. Res. May 25 2020 [PMID: 32449990] (WB, Human)
• Li L, Hu X, Eid JE et al. Mutant IDH1 Depletion Downregulates Integrins and Impairs Chondrosarcoma Growth Cancers (Basel) Jan 6 2020 [PMID: 31935911] (Human)
• Laurenzana A, Margheri F, Biagioni A, et al EGFR/uPAR interaction as druggable target to overcome vemurafenib acquired resistance in melanoma cells EBioMedicine. 2019 Jan 02 [PMID: 30611716] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: ITGA5