Glucagon Antibody (181402) [Unconjugated]

Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu, Mu
• Applications: CyTOF-ready, Flow, ICC/IF
• Clone: 181402
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Glucagon Antibody (181402) [Unconjugated] Summary

• Immunogen: Human Glucagon synthetic peptide
• Specificity: Detects human and mouse Glucagon.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GCG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glucagon Antibody (181402) [Unconjugated]

• GCG
• glicentin-related polypeptide
• GLP1
• GLP-1
• Glucagon
• glucagon-like peptide 1
• glucagon-like peptide 2
• GRPP

Background

Glucagon is a 29 amino acid residue pancreatic peptide hormone. It stimulates gluconeogenesis and glycogenolysis to counter the glucose-lowering action of insulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glucagon Antibody (MAB1249)(16)

Publications using MAB1249

• Ng, XW;DiGruccio, MR;Kong, C;Lee, J;Piston, DW; Role of Complexin 2 in the regulation of hormone secretion from the islet of Langerhans bioRxiv : the preprint server for biology 2024-10-30 [PMID: 39554053] (Immunohistochemistry, Mouse)
• Asadi, F;Gunawardana, SC;Dolle, RE;Piston, DW; An orally available compound suppresses glucagon hypersecretion and normalizes hyperglycemia in type 1 diabetes JCI insight 2024-01-23 [PMID: 38258903] (Immunofluorescence, Human)
• Lyu, X;Rowley, MJ;Kulik, MJ;Dalton, S;Corces, VG; Regulation of CTCF loop formation during pancreatic cell differentiation Nature communications 2023-10-09 [PMID: 37813869] (Flow Cytometry, Human)
• M Kohata, J Imai, T Izumi, J Yamamoto, Y Kawana, A Endo, H Sugawara, J Seiko, H Kubo, H Kawamura, T Sato, S Osaka, Y Munakata, Y Asai, S Kodama, K Takahashi, K Kaneko, H Katagiri Roles of FoxM1-driven basal beta-cell proliferation in maintenance of beta-cell mass and glucose tolerance during adulthood Journal of diabetes investigation, 2022-07-15;0(0):. 2022-07-15 [PMID: 35633298] (IHC, Mouse)
• Y Huang, E Motta, C Nanvuma, LD Kuhrt, Y Yuan, P Xia, M Lubas, S Zhu, M Schnauss, N Qazi, F Hu, H Zhang, T Lei, M Synowitz, C Flüh, H Kettenmann Microglia/macrophage-derived human CCL18 promotes glioma progression via CCR8-ACP5 axis analyzed in humanized slice model Cell Reports, 2022-04-12;39(2):110670. 2022-04-12 [PMID: 35417708] (Neutralization, Mouse)
• Laura Nigi, Noemi Brusco, Giuseppina E. Grieco, Daniela Fignani, Giada Licata, Caterina Formichi et al. Increased Expression of Viral Sensor MDA5 in Pancreatic Islets and in Hormone-Negative Endocrine Cells in Recent Onset Type 1 Diabetic Donors Frontiers in Immunology 3/11/2022 [PMID: 35359976]
• XX Yu, WL Qiu, L Yang, YC Wang, MY He, D Wang, Y Zhang, LC Li, J Zhang, Y Wang, CR Xu Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans Cell Research, 2021-03-10;0(0):. 2021-03-10 [PMID: 33692492] (IHC, Mouse)
• Laura Nigi, Noemi Brusco, Giuseppina E. Grieco, Giada Licata, Lars Krogvold, Lorella Marselli et al. Pancreatic Alpha-Cells Contribute Together With Beta-Cells to CXCL10 Expression in Type 1 Diabetes Front Endocrinol (Lausanne) 9/15/2020 [PMID: 33042009]
• P Rattanaamn, YM Roshorm, C Wilasrusme, N Proprom, B Ongphiphad, SC Talchai Direct suppression of human islet dedifferentiation, progenitor genes, but not epithelial to mesenchymal transition by liraglutide Heliyon, 2020-09-15;6(9):e04951. 2020-09-15 [PMID: 32995630] (Flow Cytometry, Human)
• F Vecchio, N Lo Buono, A Stabilini, L Nigi, MJ Dufort, S Geyer, PM Rancoita, F Cugnata, A Mandelli, A Valle, P Leete, F Mancarella, PS Linsley, L Krogvold, KC Herold, HE Larsson, SJ Richardson, NG Morgan, K Dahl-Jørge, G Sebastiani, F Dotta, E Bosi, M Battaglia Abnormal neutrophil signature in the blood and pancreas of presymptomatic and symptomatic type 1 diabetes JCI Insight, 2018-09-20;3(18):. 2018-09-20 [PMID: 30232284] (IHC-P, Human)
• Gomez D, O'Driscoll M, Sheets T, Hruban R, Oberholzer J, McGarrigle J, Shamblott M Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate. PLoS ONE, 2015-08-19;10(8):e0133862. 2015-08-19 [PMID: 26288179] (IHC, Human)
• Kornete , Mara, Beauchemin , Hugues, Polychronakos , Constant, Piccirillo , Ciriaco Pancreatic islet cell phenotype and endocrine function throughout diabetes development in non-obese diabetic mice. Autoimmunity, 2013-02-04;46(4):259-68. 2013-02-04 [PMID: 23256897] (Flow Cytometry, Mouse)
• P. Marchetti, R. Lupi, M. Bugliani, C. L. Kirkpatrick, G. Sebastiani, F. A. Grieco et al. A local glucagon-like peptide 1 (GLP-1) system in human pancreatic islets Diabetologia 12/1/2012 [PMID: 22965295]
• P. Marchetti, R. Lupi, M. Bugliani, C. L. Kirkpatrick, G. Sebastiani, F. A. Grieco et al. A local glucagon-like peptide 1 (GLP-1) system in human pancreatic islets Diabetologia 2012-12-01 [PMID: 22965295] (Immunohistochemistry, Human)
• Carnegie JR, Robert-Cooperman CE, Wu J et al. Characterization of the expression, localization, and secretion of PANDER in alpha-cells. Mol Cell Endocrinol 8/30/2010 [PMID: 20638985]
• Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U Stem cells derived from human fetal membranes display multilineage differentiation potential. Biol. Reprod., 2007-05-09;77(3):577-88. 2007-05-09 [PMID: 17494917] (ICC, Human)

Review for Glucagon Antibody (MAB1249) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 02/04/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 22069255
• Species: Mouse

Bioinformatics

• Gene Symbol: GCG