Human Leptin Quantikine ELISA Kit

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Human Leptin Quantikine ELISA Kit Summary

Background
The Quantikine Human Leptin Immunoassay is a 3.5 hour solid phase ELISA designed to measure soluble human Leptin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Leptin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant Leptin accurately. Results obtained measuring natural huma...n Leptin showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Leptin.
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Specificity
Natural and recombinant human Leptin
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
LEP

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Interference observed with 1 or more available related molecules.
Publications
Read Publications using DLP00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Leptin Quantikine ELISA Kit

  • FLJ94114
  • LEP
  • leptin (murine obesity homolog)
  • leptin (obesity homolog, mouse)
  • Leptin
  • OB
  • Obese protein
  • obese, mouse, homolog of
  • Obesity factor
  • OBOBS

Background

Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. The expression of leptin mRNA is restricted to adipose tissue.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Leptin/OB (DLP00)(125)

We have publications tested in 2 confirmed species: Human, Mouse.


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Human
(124)
Mouse
(1)
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Showing Publications 1 - 10 of 125. Show All 125 Publications.
Publications using DLP00 Applications Species
P Kittiskuln, A Banjongjit, K Metta, K Tiranathan, Y Avihingsan, K Praditporn, K Tungsanga, S Eiam-Ong The beneficial effects of intradialytic parenteral nutrition in hemodialysis patients with protein energy wasting: a prospective randomized controlled trial Scientific Reports, 2022;12(1):4529. 2022 [PMID: 35296793] (Human) Human
X Peng, J Huang, H Zou, B Peng, S Xia, K Dong, N Sun, J Tao, Y Yang Roles of plasma leptin and resistin in novel subgroups of type 2 diabetes driven by cluster analysis Lipids in Health and Disease, 2022;21(1):7. 2022 [PMID: 34996484] (Human) Human
T Jagric The Contribution of the Hunger Hormone Leptin in the Aetiology of Postoperative Anorexia after Laparoscopic and Open Gastrectomy in Gastric Cancer Patients Biomolecules, 2021;11(11):. 2021 [PMID: 34827598] (Human) Human
MT Guagnano, C D'Angelo, D Caniglia, P Di Giovann, E Celletti, E Sabatini, L Speranza, M Bucci, F Cipollone, R Paganelli Improvement of Inflammation and Pain after Three Months' Exclusion Diet in Rheumatoid Arthritis Patients Nutrients, 2021;13(10):. 2021 [PMID: 34684536] (Human) Human
F Louis, Y Sowa, S Kitano, M Matsusaki High-throughput drug screening models of mature adipose tissues which replicate the physiology of patients' Body Mass Index (BMI) Bioactive materials, 2022;7(0):227-241. 2022 [PMID: 34466729] (Human) Human
P Duriez, IAK Nilsson, O Le Thuc, D Alexandre, N Chartrel, C Rovere, C Chauveau, P Gorwood, V Tolle, O Viltart Exploring the Mechanisms of Recovery in Anorexia Nervosa through a Translational Approach: From Original Ecological Measurements in Human to Brain Tissue Analyses in Mice Nutrients, 2021;13(8):. 2021 [PMID: 34444945] (Human) Human
I Thomas, I Panagoulia, I Aggeletopo, A Varvarigou, BE Spiliotis, A Mouzaki The Role of Leptin in Childhood Immune Thrombocytopenia (ITP): An Anti-Inflammatory Agent? International Journal of Molecular Sciences, 2021;22(14):. 2021 [PMID: 34299256] (Mouse) Mouse
A Stochmal, A Wa?kiel-Bu, S Chrostowsk, M Zaremba, A Rakowska, J Czuwara, L Rudnicka Adiponectin as a novel biomarker of disease severity in alopecia areata Scientific Reports, 2021;11(1):13809. 2021 [PMID: 34226603] (Human) Human
U Andersson-, H de Maré, F Askeli, M Börjesson, A Holmäng Physical activity during pregnancy and association with changes in fat mass and adipokines in women of normal-weight or with obesity Scientific Reports, 2021;11(1):12549. 2021 [PMID: 34131242] (Human) Human
GP Caviglia, A Armandi, C Rosso, S Gaia, S Aneli, E Rolle, ML Abate, A Olivero, A Nicolosi, M Guariglia, DG Ribaldone, P Carucci, GM Saracco, E Bugianesi Biomarkers of Oncogenesis, Adipose Tissue Dysfunction and Systemic Inflammation for the Detection of Hepatocellular Carcinoma in Patients with Nonalcoholic Fatty Liver Disease Cancers, 2021;13(10):. 2021 [PMID: 34064999] (Human) Human
Show All 125 Publications.

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Bioinformatics

Gene Symbol LEP