Human Leptin Quantikine ELISA Kit



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Reactivity HuSpecies Glossary
Applications ELISA

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Human Leptin Quantikine ELISA Kit Summary

The Quantikine Human Leptin Immunoassay is a 3.5 hour solid phase ELISA designed to measure soluble human Leptin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Leptin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant Leptin accurately. Results obtained measuring natural huma...n Leptin showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Leptin.
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Natural and recombinant human Leptin
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
Interference observed with 1 or more available related molecules.
Read Publications using DLP00.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Leptin Quantikine ELISA Kit

  • FLJ94114
  • LEP
  • leptin (murine obesity homolog)
  • leptin (obesity homolog, mouse)
  • Leptin
  • OB
  • Obese protein
  • obese, mouse, homolog of
  • Obesity factor


Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. The expression of leptin mRNA is restricted to adipose tissue.

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Applications: WB, ELISA, IHC, Neut
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Applications: IHC, IHC-P
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Species: Hu, Mu, Rt
Applications: WB
Species: Mu, Rt
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for Leptin/OB (DLP00)(104)

We have publications tested in 1 confirmed species: Human.

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Showing Publications 1 - 10 of 104. Show All 104 Publications.
Publications using DLP00 Applications Species
O Hakim, O Bello, M Ladwa, JL Peacock, AM Umpleby, G Charles-Ed, SA Amiel, LM Goff The Link between Obesity and Inflammatory Markers in the Development of Type 2 Diabetes in Men of Black African and White European Ethnicity Nutrients, 2020;12(12):. 2020 [PMID: 33322261] (Human) Human
AE Thackray, SA Willis, DJ Clayton, DR Broom, G Finlayson, FR Goltz, JA Sargeant, RM Woods, DJ Stensel, JA King Influence of Short-Term Hyperenergetic, High-Fat Feeding on Appetite, Appetite-Related Hormones, and Food Reward in Healthy Men Nutrients, 2020;12(9):. 2020 [PMID: 32872401] (Human) Human
T Sarabhai, S Kahl, J Szendroedi, DF Markgraf, OP Zaharia, C Barosa, C Herder, F Wickrath, P Bobrov, JH Hwang, JG Jones, M Roden Monounsaturated fat rapidly induces hepatic gluconeogenesis and whole-body insulin resistance JCI Insight, 2020;5(10):. 2020 [PMID: 32434996] (Human) Human
J Gorwood, T Ejlalmanes, C Bourgeois, M Mantecon, C Rose, M Atlan, D Desjardins, R Le Grand, B Fève, O Lambotte, J Capeau, V Béréziat, C Lagathu SIV Infection and the HIV Proteins Tat and Nef Induce Senescence in Adipose Tissue and Human Adipose Stem Cells, Resulting in Adipocyte Dysfunction Cells, 2020;9(4):. 2020 [PMID: 32244726] (Human) Human
A Basolo, M Hohenadel, QY Ang, P Piaggi, S Heinitz, M Walter, P Walter, S Parrington, DD Trinidad, RJ von Schwar, PJ Turnbaugh, J Krakoff Effects of underfeeding and oral vancomycin on gut microbiome and nutrient absorption in humans Nat. Med., 2020;26(4):589-598. 2020 [PMID: 32235930] (Human) Human
ED Sims, WJ Jennings, B Empringham, A Fleming, C Portwine, DL Johnston, SM Zelcer, SR Rassekh, S Burrow, L Thabane, MC Samaan Circulating leptin levels are associated with adiposity in survivors of childhood brain tumors Sci Rep, 2020;10(1):4711. 2020 [PMID: 32170116] (Human) Human
R Sámano, H Martínez-R, G Chico-Barb, M Hernández-, R Guzmán, G Arteaga-Tr, MA Figueroa-P, RM Morales, G Martínez Associations between prenatal serum levels of leptin, IGF-I, and estradiol and adolescent mothers' height gain during and after pregnancy PLoS ONE, 2020;15(2):e0228706. 2020 [PMID: 32045435] (Human) Human
AE O'Mara, JW Johnson, JD Linderman, RJ Brychta, S McGehee, LA Fletcher, YA Fink, D Kapuria, TM Cassimatis, N Kelsey, C Cero, Z Abdul-Sate, F Piccinini, AS Baskin, BP Leitner, H Cai, CM Millo, W Dieckmann, M Walter, NB Javitt, Y Rotman, PJ Walter, M Ader, RN Bergman, P Herscovitc, KY Chen, AM Cypess Chronic mirabegron treatment increases human brown fat, HDL cholesterol, and insulin sensitivity J. Clin. Invest., 2020;0(0):. 2020 [PMID: 31961826] (Human) Human
T Sawaguchi, T Nakajima, A Haruyama, T Hasegawa, I Shibasaki, T Nakajima, H Kaneda, T Arikawa, S Obi, M Sakuma, H Ogawa, Y Takei, S Toyoda, F Nakamura, S Abe, H Fukuda, T Inoue Association of serum leptin and adiponectin concentrations with echocardiographic parameters and pathophysiological states in patients with cardiovascular disease receiving cardiovascular surgery PLoS ONE, 2019;14(11):e0225008. 2019 [PMID: 31703113] (Human) Human
L Shen, JF Cordero, JS Wang, Y Shen, S Li, L Liang, Z Zou, C Li Association between genetically determined leptin and blood lipids considering alcohol consumption: a Mendelian randomisation study BMJ Open, 2019;9(11):e026860. 2019 [PMID: 31699712] (Human) Human
Show All 104 Publications.

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FAQs for Leptin/OB (DLP00). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Gene Symbol LEP