Rat IgG2a Isotype Control (54447) [Unconjugated]

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B16-F1 mouse melanoma cell line was stained with RatAnti-Mouse Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405,filled histogram) or Rat IgG Isotype Control Antibody (Catalog #MAB006,open histogram), ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Ctrl
Clone
54447
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Rat IgG2a Isotype Control (54447) [Unconjugated] Summary

Immunogen
KLH
Specificity
Selected for use as rat IgG2A isotype control. In direct ELISAs, does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhACT II (rhMIP-1 beta ), rhBLC/BCA-1, rmBLC, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhCK beta 8-1, recombinant viral (rv) CMV UL146, rmCRG-2, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin 2, rhEotaxin-3, rmEotaxin 2, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, recombinant porcine IL-8, rhIP-10, rhI-TAC, rmJE, rmKC, rhLeukotactin -1, rhLymphytactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rvMCV Type 2, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-1 gamma, rhMIP-1δ, rmMIP-2, rhMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 alpha, rmMIP-3 beta, rvMIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhParc, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTarc, rmTARC, rmTCA-3, rhTeck, or rmTeck.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

Reviewed Applications
Read 1 Review rated 5
using
MAB006 in the following application:

Publications
Read Publications using
MAB006 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IgG2a Isotype Control (54447) [Unconjugated]

  • IgG2A

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2a Isotype Control (MAB006)(105)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 12 applications: Bioassay, Bioassay Control, Control, ELISA Development, Flow Cytometry, Flow Cytometry Control, IHC, IHC-Fr, In Vivo, Isotype Control, Neutralization, Neutralization Control.


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Bioassay
(1)
Bioassay Control
(1)
Control
(44)
ELISA Development
(1)
Flow Cytometry
(10)
Flow Cytometry Control
(2)
IHC
(4)
IHC-Fr
(2)
In Vivo
(3)
Isotype Control
(6)
Neutralization
(19)
Neutralization Control
(5)
All Applications
Filter By Species
Human
(8)
Mouse
(92)
Rat
(2)
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Showing Publications 1 - 10 of 105. Show All 105 Publications.
Publications using MAB006 Applications Species
S An, I Raju, B Surenkhuu, JE Kwon, S Gulati, M Karaman, A Pradeep, S Sinha, C Mun, S Jain Neutrophil extracellular traps (NETs) contribute to pathological changes of ocular graft-vs.-host disease (oGVHD) dry eye: Implications for novel biomarkers and therapeutic strategies Ocul Surf, 2019;0(0):. 2019 [PMID: 30965123] (Isotype Control, Human) Isotype Control Human
S Mukai, Y Ogawa, H Saya, Y Kawakami, K Tsubota Therapeutic potential of tranilast for the treatment of chronic graft-versus-host disease in mice PLoS ONE, 2018;13(10):e0203742. 2018 [PMID: 30307955] (Isotype Control, Mouse) Isotype Control Mouse
BL Hoh, K Rojas, L Lin, HZ Fazal, S Hourani, KW Nowicki, MB Schneider, K Hosaka Estrogen Deficiency Promotes Cerebral Aneurysm Rupture by Upregulation of Th17 Cells and Interleukin-17A Which Downregulates E-Cadherin J Am Heart Assoc, 2018;7(8):. 2018 [PMID: 29654199] (Neutralization, Mouse) Neutralization Mouse
X Shi, S Wei, KJ Simms, DN Cumpston, TJ Ewing, P Zhang Sonic Hedgehog Signaling Regulates Hematopoietic Stem/Progenitor Cell Activation during the Granulopoietic Response to Systemic Bacterial Infection Front Immunol, 2018;9(0):349. 2018 [PMID: 29535725] (Flow Cytometry, Mouse) Flow Cytometry Mouse
S Mukai, Y Ogawa, Y Kawakami, Y Mashima, K Tsubota Inhibition of Vascular Adhesion Protein-1 for Treatment of Graft-Versus-Host Disease in Mice FASEB J., 2018;0(0):fj201700176R. 2018 [PMID: 29490166] (IHC-Fr, Mouse) IHC-Fr Mouse
JM Gu, S Yuan, D Sim, K Abe, P Liu, M Rosenbruch, P Bringmann, K Kauser Blockade of placental growth factor reduces vaso-occlusive complications in murine models of sickle cell disease Exp. Hematol., 2018;0(0):. 2018 [PMID: 29337222] (Neutralization, Mouse) Neutralization Mouse
A Okuma, A Hanyu, S Watanabe, E Hara p16Ink4a and p21Cip1/Waf1 promote tumour growth by enhancing myeloid-derived suppressor cells chemotaxis Nat Commun, 2017;8(1):2050. 2017 [PMID: 29234059] (In Vivo, Mouse) In Vivo Mouse
I Flamme, P Ellinghaus, D Urrego, T Krüger FGF23 expression in rodents is directly induced via erythropoietin after inhibition of hypoxia inducible factor proline hydroxylase PLoS ONE, 2017;12(10):e0186979. 2017 [PMID: 29073196] (Control, Rat) Control Rat
H Matsumoto, N Matsumoto, J Shimazaki, J Nakagawa, Y Imamura, K Yamakawa, T Yamada, M Ikeda, H Hiraike, H Ogura, T Shimazu Therapeutic Effectiveness of Anti-RAGE Antibody Administration in a Rat Model of Crush Injury Sci Rep, 2017;7(1):12255. 2017 [PMID: 28947744] (IHC, Rat) IHC Rat
K Watanabe, CA Gilchrist, MJ Uddin, SL Burgess, MM Abhyankar, SN Moonah, Z Noor, JR Donowitz, BN Schneider, T Arju, E Ahmed, M Kabir, M Alam, R Haque, P Pramoonjag, B Mehrad, WA Petri Microbiome-mediated neutrophil recruitment via CXCR2 and protection from amebic colitis PLoS Pathog., 2017;13(8):e1006513. 2017 [PMID: 28817707] (Neutralization, Mouse) Neutralization Mouse
Show All 105 Publications.

Review for IgG2a Isotype Control (MAB006) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using MAB006:
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ICC/IF
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry/Immunofluorescence IgG2a MAB006
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reviewed by:
Anonymous
ICC/IF 06/13/2017
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
LotCA02315081

FAQs for IgG2a Isotype Control (MAB006) (0)

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Anonymous
06/13/2017
Application: ICC/IF
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