IL-3R alpha/CD123 Antibody (6H6)

Images

 
Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Analysis using the PE/Cy7 conjugate of NB600-1185. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy7 ...read more
Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Staining of TF-1 cells with Purified Mouse IgG1, K Iso Cntrl (open histogram) or Purified anti-human CD123 (6H6) (colored histogram) followed by PE ...read more
Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy7 (NBP1-42944) (open histogram) or Anti-Human CD123 PE-Cy7 ...read more
Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Staining of normal human peripheral blood cells with Anti-Human CD85g (ILT7) Alexa Fluor 647 and Mouse IgG1 kappa Isotype Control PerCP-Cy5.5 (NBP1-42839) ...read more
Flow (Intracellular): IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Analysis using the DyLight 488 conjugate of NB600-1185. Staining of Human monocytes were probed using 10 ul of CD123 antibody (right) or 0.05 ug of ...read more
Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Analysis using the DyLight 405 conjugate of NB600-1185. Staining of CD123 in human PBMCs using anti-CD123 antibody. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-IC, IHC, IHC-Fr
Clone
6H6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

IL-3R alpha/CD123 Antibody (6H6) Summary

Immunogen
Tissue / cell preparation. IL3R transfected COS cells.
Localization
Type I membrane protein.
Specificity
IL3RA (6H6)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IL3RA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Flow (Intracellular) 1:10 - 1:1000
Application Notes
Flow Cytometry Use 1ug for 10^6 cells in 100 ul volume or 100 ul of whole blood. This antibody has been tested by tested by immunofluorescent staining with flow cytometric analysis. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Although not tested this antibody may be useful in IHC-P and Immunoprecipitation. WB was reported in the scientific literature (PMID:12576313).
Publications
Read Publications using
NB600-1185 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.2)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for IL-3R alpha/CD123 Antibody (6H6)

  • CD123 antigen
  • CD123
  • hIL3Ra
  • hIL-3Ra
  • IL-3 R alpha
  • IL-3 receptor subunit alpha
  • IL3R alpha
  • IL-3R alpha
  • IL-3R subunit alpha
  • IL3R
  • IL3RA
  • IL-3Ra
  • IL-3R-alpha
  • IL3RAY
  • IL3RX
  • IL3RY
  • interleukin 3 receptor, alpha (low affinity)
  • interleukin-3 receptor subunit alpha
  • MGC34174

Background

IL3RA (CD123) is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either common beta chain (CDw131) or the IL3RB chain to form high affinity heterodimeric IL3 receptors. CDw131 can complex with the Alpha subunits of the mouse IL3R, IL5R and GMCSFR to form high affinity receptors, while the IL3RB subunit is specific for IL3 but binds with low affinity. IL3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. It is present in Hematopoietic progenitors, basophils, mast cells and megakaryocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-3R alpha/CD123 Antibody (NB600-1185)(3)

Reviews for IL-3R alpha/CD123 Antibody (NB600-1185) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IL-3R alpha/CD123 Antibody (NB600-1185). (Showing 1 - 1 of 1 FAQs).

  1. We have an inquiry of your product Cat. ID # NB100-2735, NB600-1185, NB100-77758, NB100-78016, NB100-77855. We are interested in this product and would like to make sure that there is no any other protein added in the buffer. I saw the storage buffer in datasheet doesn't has any other protein but still would like to confirm with you again. Would you please help us to check and provide the purification method and buffer of the antibody?
    • Thank you for your inquiry. Catalog numbers NB100-77758, NB100-78016 and NB100-77855 are in a buffer containing PBS and sodium azide. NB600-1185 is in a buffer containing PBS, 150mM NaCl and sodium azide. NB100-2735 is whole tissue culture supernatant and may therefore contain other proteins.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-1185AF350
Alexa Fluor 405 NB600-1185AF405
Alexa Fluor 488 NB600-1185AF488
Alexa Fluor 532 NB600-1185AF532
Alexa Fluor 594 NB600-1185AF594
Alexa Fluor 647 NB600-1185AF647
Alexa Fluor 700 NB600-1185AF700
Alexa Fluor 750 NB600-1185AF750
Allophycocyanin NB600-1185APC
Allophycocyanin/Cy7 NB600-1185APCCY7
Biotin NB100-2482
DyLight 350 NB600-1185UV
DyLight 405 NB600-1185V
DyLight 488 NB600-1185G
DyLight 550 NB600-1185R
DyLight 594 NB600-1185DL594
DyLight 650 NB600-1185C
DyLight 680 NB600-1185FR
DyLight 755 NB600-1185IR
Janelia Fluor 549 NB600-1185JF549
Janelia Fluor 646 NB600-1185JF646
PE NB600-1185PE
PE/Atto594 NB600-1185PEATT594
PE/Cy5.5 NB600-1185PECY55
PE/Cy7 NB600-1185PECY7
PE/Cy7 NBP2-00467
PerCP NB600-1185PCP
PerCP/Cy5.5 NBP2-00468

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Bioinformatics

Gene Symbol IL3RA
Entrez