IL-3R alpha/CD123 Antibody (6H6) - BSA Free

Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Analysis using the PE/Cy7 conjugate of NB600-1185. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy7 (NBP1-42944) (open histogram) or Anti-Human CD123 PE-Cy7 (filled histogram). Cells in the lymphocyte gate were

Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Staining of TF-1 cells with Purified Mouse IgG1, K Iso Cntrl (open histogram) or Purified anti-human CD123 (6H6) (colored histogram) followed by PE Anti-Mouse IgG. Total viable cells were used for analysis

Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy7 (NBP1-42944) (open histogram) or Anti-Human CD123 PE-Cy7 (filled histogram). Cells in the lymphocyte gate were used for analysis.

Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Staining of normal human peripheral blood cells with Anti-Human CD85g (ILT7) Alexa Fluor 647 and Mouse IgG1 kappa Isotype Control PerCP-Cy5.5 (NBP1-42839) (left) or Anti-Human CD123 PerCP-Cy5.5 (right). Cells in the monocyte gate were used for analysis.

Flow (Intracellular): IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Analysis using the DyLight 488 conjugate of NB600-1185. Staining of Human monocytes were probed using 10 ul of CD123 antibody (right) or 0.05 ug of isotype control was used for this test.

Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [NB600-1185] - Analysis using the DyLight 405 conjugate of NB600-1185. Staining of CD123 in human PBMCs using anti-CD123 antibody. Image from verified customer review.

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clone: 6H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

IL-3R alpha/CD123 Antibody (6H6) - BSA Free Summary

• Immunogen: Tissue / cell preparation. IL3R transfected COS cells.
• Localization: Type I membrane protein.
• Specificity: IL3RA (6H6)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL3RA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular) 1:10 - 1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Flow Cytometry Use 1ug for 10^6 cells in 100 ul volume or 100 ul of whole blood. This antibody has been tested by tested by immunofluorescent staining with flow cytometric analysis. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Although not tested this antibody may be useful in IHC-P and Immunoprecipitation. WB was reported in the scientific literature (PMID:12576313).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for IL-3R alpha/CD123 Antibody (6H6) - BSA Free

• CD123 antigen
• CD123
• hIL3Ra
• hIL-3Ra
• IL-3 R alpha
• IL-3 receptor subunit alpha
• IL3R alpha
• IL-3R alpha
• IL-3R subunit alpha
• IL3R
• IL3RA
• IL-3Ra
• IL-3R-alpha
• IL3RAY
• IL3RX
• IL3RY
• interleukin 3 receptor, alpha (low affinity)
• interleukin-3 receptor subunit alpha
• MGC34174

Background

IL3RA (CD123) is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either common beta chain (CDw131) or the IL3RB chain to form high affinity heterodimeric IL3 receptors. CDw131 can complex with the Alpha subunits of the mouse IL3R, IL5R and GMCSFR to form high affinity receptors, while the IL3RB subunit is specific for IL3 but binds with low affinity. IL3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. It is present in Hematopoietic progenitors, basophils, mast cells and megakaryocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-3R alpha/CD123 Antibody (NB600-1185)(4)

Publications using NB600-1185

• Yu J, Thomas P, Sciacca M et al. Ad26.COV2.S and SARS-CoV-2 Spike Protein Ferritin Nanoparticle Vaccine Protect Against SARS-CoV-2 Omicron BA.5 Challenge in Macaques Cell Reports Medicine 2023-03-01 [PMID: 37023746] (ICC/IF, Primate)
• Ligresti G, Caporarello N, Meridew JA et al. Primary intestinal NK-cell lymphoma, EBV-negative: A case report and literature review Human Pathology: Case Reports 2019-09-01 (IF/IHC, Human)
• Chung T, Christopher-Stine L, Paik JJ et al. The composition of cellular infiltrates in anti-HMG-CoA reductase-associated myopathy Muscle Nerve 2015-03-03 [PMID: 25737145] (IHC-Fr, Human)
  
  Details:
  IL-3 R alpha/CD123 antibody used in IHC-Fr application for immunostaining of CD123 (the plasmacytoid dendritic cell phenotypic marker) on samples from human anti-3-hydroxy-3-methyl-gulatryl-CoA reductase (HMGCR)-associated myopathy patients and control dermatomyositis patients. The assay involved - 7 um thick frozen muscle biopsy sections, washing of sections with tris buffer pH 7.2 followed by incubation with 1:100 dilution for 1 hour at RT, detection with HRP-conjugated secondary antibody and DAB chromogen (Figure 3).
• Dunphy CH. Comparative analysis of detecting monocytic cells and their aberrancy. Appl Immunohistochem Mol Morphol;19(4):336-40. 2011-07-01 [PMID: 21293259] (IF/IHC, Human)

FAQs for IL-3R alpha/CD123 Antibody (NB600-1185). (Showing 1 - 1 of 1 FAQs).

1. We have an inquiry of your product Cat. ID # NB100-2735, NB600-1185, NB100-77758, NB100-78016, NB100-77855. We are interested in this product and would like to make sure that there is no any other protein added in the buffer. I saw the storage buffer in datasheet doesn't has any other protein but still would like to confirm with you again. Would you please help us to check and provide the purification method and buffer of the antibody?
   • Thank you for your inquiry. Catalog numbers NB100-77758, NB100-78016 and NB100-77855 are in a buffer containing PBS and sodium azide. NB600-1185 is in a buffer containing PBS, 150mM NaCl and sodium azide. NB100-2735 is whole tissue culture supernatant and may therefore contain other proteins.

Bioinformatics

• Gene Symbol: IL3RA
• Entrez:
  • 3563(Human)