Recombinant Human IL-3 Protein

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1 μg/lane of Recombinant Human IL-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human IL-3 (Catalog # 203-IL) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.02-0.1 ng/mL.

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-3 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.02-0.1 ng/mL.
The specific activity of Recombinant Human IL-3 is approximately 1.8 x 103 IU/μg, which is calibrated against recombinant human IL-3 WHO International Standard (NIBSC code: 91/510).
Source
E. coli-derived human IL-3 protein
Ala20-Phe152, with and without an N-terminal Met
Accession #
N-terminal Sequence
Ala20 and Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL3
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
203-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-3 Protein

  • Hematopoietic growth factor
  • IL3
  • IL-3
  • IL-3MGC79398
  • interleukin 3 (colony-stimulating factor, multiple)
  • interleukin-3
  • Mast cell growth factor
  • mast-cell growth factor
  • MCGFMGC79399
  • MULTI-CSF
  • multilineage-colony-stimulating factor
  • Multipotential colony-stimulating factor
  • P-cell stimulating factor
  • P-cell-stimulating factor

Background

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the  alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

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Publications for IL-3 (203-IL)(131)

We have publications tested in 6 confirmed species: Human, Mouse, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Xenograft.

We have publications tested in 4 applications: Bioassay, Cell Culture, Differentiation, In Vivo.


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Bioassay
(128)
Cell Culture
(1)
Differentiation
(1)
In Vivo
(1)
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Human
(123)
Mouse
(4)
Porcine
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Xenograft
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Showing Publications 1 - 10 of 131. Show All 131 Publications.
Publications using 203-IL Applications Species
K Trakarnsan, D Ferguson, DE Daniels, RE Griffiths, MC Wilson, KE Mordue, A Gartner, TN Andrienko, A Calvert, A Condie, A McCahill, JC Mountford, AM Toye, DJ Anstee, J Frayne Vimentin expression is retained in erythroid cells differentiated from human iPSC and ESC and indicates dysregulation in these cells early in differentiation Stem Cell Res Ther, 2019;10(1):130. 2019 [PMID: 31036072] (Bioassay, Human) Bioassay Human
B Mair, J Tomic, SN Masud, P Tonge, A Weiss, M Usaj, AHY Tong, JJ Kwan, KR Brown, E Titus, M Atkins, KSK Chan, L Munsie, A Habsid, H Han, M Kennedy, B Cohen, G Keller, J Moffat Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies Cell Rep, 2019;27(2):599-615.e12. 2019 [PMID: 30970261] (Differentiation, Human) Differentiation Human
AK Seneviratn, M Xu, JJA Henao, VA Fajardo, Z Hao, V Voisin, GW Xu, R Hurren, S Kim, N MacLean, X Wang, M Gronda, D Jeyaraju, Y Jitkova, T Ketela, M Mullokando, D Sharon, G Thomas, R Chouinard-, JR Hawley, C Schafer, HL Yau, Z Khuchua, A Aman, R Al-Awar, A Gross, SM Claypool, R Bazinet, M Lupien, S Chan, DD De Carvalh, MD Minden, GD Bader, KD Stark, P LeBlanc, AD Schimmer The Mitochondrial Transacylase, Tafazzin, Regulates for AML Stemness by Modulating Intracellular Levels of Phospholipids Cell Stem Cell, 2019;0(0):. 2019 [PMID: 30930145] (Bioassay, Human) Bioassay Human
H Xu, B Wang, M Ono, A Kagita, K Fujii, N Sasakawa, T Ueda, P Gee, M Nishikawa, M Nomura, F Kitaoka, T Takahashi, K Okita, Y Yoshida, S Kaneko, A Hotta Targeted Disruption of HLA Genes via CRISPR-Cas9 Generates iPSCs with Enhanced Immune Compatibility Cell Stem Cell, 2019;0(0):. 2019 [PMID: 30853558] (Bioassay, Human) Bioassay Human
M Suga, T Kondo, K Imamura, R Shibukawa, Y Okanishi, Y Sagara, K Tsukita, T Enami, M Furujo, K Saijo, Y Nakamura, M Osawa, MK Saito, S Yamanaka, H Inoue Generation of a human induced pluripotent stem cell line, BRCi001-A, derived from a patient with mucopolysaccharidosis type I Stem Cell Res, 2019;36(0):101406. 2019 [PMID: 30849633] (Bioassay, Human) Bioassay Human
SL Taylor, J Hogwood, W Guo, EA Yates, JE Turnbull By-Products of Heparin Production Provide a Diverse Source of Heparin-like and Heparan Sulfate Glycosaminoglycans Sci Rep, 2019;9(1):2679. 2019 [PMID: 30804383] (Bioassay, Mouse) Bioassay Mouse
S Vangeti, J Gertow, M Yu, S Liu, F Baharom, S Scholz, D Friberg, M Starkhamma, A Ahlberg, A Smed-Sören Human Blood and Tonsil Plasmacytoid Dendritic Cells Display Similar Gene Expression Profiles but Exhibit Differential Type I IFN Responses to Influenza A Virus Infection J. Immunol., 2019;0(0):. 2019 [PMID: 30760619] (Bioassay, Human) Bioassay Human
P Potirat, M Wattanapan, V Viprakasit, P Kheolamai, S Issaragris An integration-free iPSC line (MUSIi008-A) derived from a patient with severe hemolytic anemia carrying compound heterozygote mutations in KLF1 gene for disease modeling Stem Cell Res, 2019;34(0):101344. 2019 [PMID: 30605837] (Bioassay, Human) Bioassay Human
S Poldee, C Metheetrai, S Nugoolsuks, J Frayne, K Trakarnsan Optimization of an erythroid culture system to reduce the cost of in vitro production of red blood cells MethodsX, 2018;5(0):1626-1632. 2018 [PMID: 30560064] (Bioassay, Human) Bioassay Human
A Hyder, S Ehnert, F Fändrich, H Ungefroren Transfection of Peripheral Blood Monocytes with SOX2 Enhances Multipotency, Proliferation, and Redifferentiation into Neohepatocytes and Insulin-Producing Cells Stem Cells Int, 2018;2018(0):4271875. 2018 [PMID: 30402109] (Bioassay, Human) Bioassay Human
Show All 131 Publications.

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Bioinformatics

Gene Symbol IL3
Uniprot