Recombinant Human IL-3 Protein

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Recombinant Human IL-3 (Catalog # 203-IL) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.02-0.1 ng/mL.
1 μg/lane of Recombinant Human IL-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-3 Protein Summary

Additional Information
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Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.02-0.1 ng/mL.
Source
E. coli-derived human IL-3 protein
Ala20-Phe152, with and without an N-terminal Met
Accession #
N-terminal Sequence
Ala20 and Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL3
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
203-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-3 Protein

  • Hematopoietic growth factor
  • IL3
  • IL-3
  • IL-3MGC79398
  • interleukin 3 (colony-stimulating factor, multiple)
  • interleukin-3
  • Mast cell growth factor
  • mast-cell growth factor
  • MCGF
  • MCGFMGC79399
  • MULTI-CSF
  • multilineage-colony-stimulating factor
  • Multipotential colony-stimulating factor
  • P-cell stimulating factor
  • P-cell-stimulating factor

Background

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the  alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

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203-IL
Species: Hu
Applications: Bioactivity

Publications for IL-3 (203-IL)(198)

We have publications tested in 6 confirmed species: Human, Mouse, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Xenograft.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, In Vivo, SDS-Page.


Filter By Application
Bioassay
(178)
Cell Culture
(16)
Differentiation
(2)
In Vivo
(1)
SDS-Page
(1)
All Applications
Filter By Species
Human
(188)
Mouse
(6)
Porcine
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 198. Show All 198 Publications.
Publications using 203-IL Applications Species
Gillan, JL;Chokshi, M;Hardisty, GR;Clohisey Hendry, S;Prasca-Chamorro, D;Robinson, NJ;Lasota, B;Clark, R;Murphy, L;Whyte, MKB;Baillie, JK;Davidson, DJ;Bao, G;Gray, RD; CAGE sequencing reveals CFTR-dependent dysregulation of type I IFN signaling in activated cystic fibrosis macrophages Science advances 2023-05-26 [PMID: 37235648] (Bioassay, Human) Bioassay Human
Shariatzadeh, M;Nagtzaam, NMA;van Vark-van der Zee, L;van Holten-Neelen, C;Verhoeven, AJM;Dehairs, J;Swinnen, JV;Leijten, F;Ten Berge, JC;Ciriano, JPM;Wong, KT;Mulder, M;Leenen, PJM;Dik, WA; Altered Functionality of Lipoprotein(a) Impacts on Angiogenesis in Diabetic Retinopathy Investigative ophthalmology & visual science 2023-05-01 [PMID: 37159194] (Bioassay, Human) Bioassay Human
A Di Nardo, YL Chang, S Alimohamma, K Masuda-Kur, Z Wang, K Sriram, PA Insel Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts Cell Reports, 2023-04-28;42(5):112453. 2023-04-28 [PMID: 37120813] (Bioassay, Human) Bioassay Human
AA Almad, L Garcia, A Takanohash, A Gagne, W Yang, J Ann McGuir, D French, A Vanderver Generation of three induced Pluripotent Stem Cell lines from individuals with Hypomyelination with Atrophy of Basal Ganglia and Cerebellum caused by a c.745G&gt;A (p.D249N) autosomal dominant mutation in TUBB4A Stem Cell Research, 2023-03-26;69(0):103083. 2023-03-26 [PMID: 37003180] (Bioassay, Human) Bioassay Human
JR James, J Curd, JC Ashworth, M Abuhantash, M Grundy, CH Seedhouse, KP Arkill, AJ Wright, CLR Merry, A Thompson Hydrogel-Based Pre-Clinical Evaluation of Repurposed FDA-Approved Drugs for AML International Journal of Molecular Sciences, 2023-02-20;24(4):. 2023-02-20 [PMID: 36835644] (Bioassay, Human) Bioassay Human
L Feige, T Kozaki, G Dias de Me, V Guillemot, F Larrous, F Ginhoux, H Bourhy Susceptibilities of CNS Cells towards Rabies Virus Infection Is Linked to Cellular Innate Immune Responses Viruses, 2022-12-29;15(1):. 2022-12-29 [PMID: 36680128] (Bioassay, Human) Bioassay Human
S Takase, T Hiroyama, F Shirai, Y Maemoto, A Nakata, M Arata, S Matsuoka, T Sonoda, H Niwa, S Sato, T Umehara, M Shirouzu, Y Nishigaya, T Sumiya, N Hashimoto, R Namie, M Usui, T Ohishi, SI Ohba, M Kawada, Y Hayashi, H Harada, T Yamaguchi, Y Shinkai, Y Nakamura, M Yoshida, A Ito A specific G9a inhibitor unveils BGLT3 lncRNA as a universal mediator of chemically induced fetal globin gene expression Nature Communications, 2023-01-12;14(1):23. 2023-01-12 [PMID: 36635268] (Bioassay, Human) Bioassay Human
X Wang, S Cooper, HE Broxmeyer, R Kapur Transient regulation of RNA methylation in human hematopoietic stem cells promotes their homing and engraftment Leukemia, 2022-12-02;0(0):. 2022-12-02 [PMID: 36460765] (Cell Culture, Human) Cell Culture Human
PY Su, W Lee, J Zernant, SH Tsang, T Nagasaki, B Corneo, R Allikmets Establishment of the iPSC line CUIMCi005-A from a patient with Stargardt disease for retinal organoid culture Oncogene, 2022-11-14;65(0):102973. 2022-11-14 [PMID: 36455383] (Bioassay, Human) Bioassay Human
X Liu, N Sato, T Yabushita, J Li, Y Jia, M Tamura, S Asada, T Fujino, T Fukushima, T Yonezawa, Y Tanaka, T Fukuyama, A Tsuchiya, S Shikata, H Iwamura, C Kinouchi, K Komatsu, S Yamasaki, T Shibata, AT Sasaki, J Schibler, M Wunderlich, E O'Brien, B Mizukawa, JC Mulloy, Y Sugiura, H Takizawa, T Shibata, K Miyake, T Kitamura, S Goyama IMPDH inhibition activates TLR-VCAM1 pathway and suppresses the development of MLL-fusion leukemia Embo Molecular Medicine, 2022-12-01;0(0):e15631. 2022-12-01 [PMID: 36453131] (Cell Culture, Human) Cell Culture Human
Show All 198 Publications.

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Bioinformatics

Gene Symbol IL3
Uniprot