IL-3R alpha/CD123 Antibody (6H6) [DyLight 405]

Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [DyLight 405] [NB600-1185V] - analysis of CD123 in human PBMCs using anti-CD123 antibody. Image from verified customer review.

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clone: 6H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 405

IL-3R alpha/CD123 Antibody (6H6) [DyLight 405] Summary

• Immunogen: Tissue / cell preparation. IL3R transfected COS cells.
• Localization: Type I membrane protein.
• Specificity: IL3RA (6H6)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL3RA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for IL-3R alpha/CD123 Antibody (6H6) [DyLight 405]

• CD123 antigen
• CD123
• hIL3Ra
• hIL-3Ra
• IL-3 R alpha
• IL-3 receptor subunit alpha
• IL3R alpha
• IL-3R alpha
• IL-3R subunit alpha
• IL3R
• IL3RA
• IL-3Ra
• IL-3R-alpha
• IL3RAY
• IL3RX
• IL3RY
• interleukin 3 receptor, alpha (low affinity)
• interleukin-3 receptor subunit alpha
• MGC34174

Background

IL3RA (CD123) is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either common beta chain (CDw131) or the IL3RB chain to form high affinity heterodimeric IL3 receptors. CDw131 can complex with the Alpha subunits of the mouse IL3R, IL5R and GMCSFR to form high affinity receptors, while the IL3RB subunit is specific for IL3 but binds with low affinity. IL3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. It is present in Hematopoietic progenitors, basophils, mast cells and megakaryocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for IL-3R alpha/CD123 Antibody (NB600-1185V) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 06/30/2015

Summary

• Application: Flow Cytometry
• Sample Tested: PBMCs
• Species: Human

Bioinformatics

• Gene Symbol: IL3RA