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203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-3 Ra/CD123 Monoclonal Antibody (Catalog # MAB301). The ND50 is typically 0.6-1.2 µg/mL." class="big_lightbox" target="_blank">
MAB002, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox" target="_blank">
203-IL) is present at 1.00 ug/mL, the concentration of Recombinant Human IL-3R alpha/CD123 Fc Chimera (Catalog # 11140-R3) that produces 50% of the binding response is 10.0-80.0 ng/mL." class="big_lightbox" target="_blank">
203-IL) was measured at a concentration range between 0.277 nM and 2 µM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=90.9 nM. (Biacore T200)." class="big_lightbox" target="_blank">
IL3RA (CD123) is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either common beta chain (CDw131) or the IL3RB chain to form high affinity heterodimeric IL3 receptors. CDw131 can complex with the Alpha subunits of the mouse IL3R, IL5R and GMCSFR to form high affinity receptors, while the IL3RB subunit is specific for IL3 but binds with low affinity. IL3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. It is present in Hematopoietic progenitors, basophils, mast cells and megakaryocytes.
![Immunocytochemistry IL-3R alpha/CD123 Antibody (32703) [Unconjugated]](http://images.novusbio.com/images/antibody/IL-3_R_alpha_MAB301_Immunocytochemistry_6602.jpg)
![Sandwich ELISA IL-3R alpha/CD123 Antibody Pair [HRP]](http://images.novusbio.com/fullsize/IL-3R-alpha-CD123-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79483-img0001.jpg)
![Binding Activity IL-3R alpha/CD123 [Unconjugated]](http://images.novusbio.com/images/protein/IL-3_R_alpha_301-R3_580.jpg)
![Binding Activity IL-3R alpha/CD123 [Unconjugated]](http://images.novusbio.com/images/11140-r3_recombinant-human-il-3r-alpha-cd123-fc-chimera-protein-cf-bioactivity-7122022134133.jpg)
![Binding Activity IL-3R alpha/CD123 [Unconjugated]](http://images.novusbio.com/images/protein/10597-r3_recombinant-cynomolgus-monkey-il-3r-alpha-fc-protein-cf-binding-activity-1792020101317.jpg)
![Immunohistochemistry IL-3R alpha/CD123 Antibody (32703) [Unconjugated]](http://images.novusbio.com/images/antibody/IL-3_R_alpha_MAB301_Immunohistochemistry_6988.jpg)
![Neutralization IL-3R alpha/CD123 Antibody (32703) [Unconjugated]](http://images.novusbio.com/images/antibody/IL-3_R_alpha_MAB301_Block_Neutralize_2301.jpg)
![Flow Cytometry IL-3R alpha/CD123 Antibody (32703) [Unconjugated]](http://images.novusbio.com/images/antibody/mab301_human-il-3-r-alpha-cd123-mab-clone-32703-flow-cytometry-512024183146.jpg)
![SDS-Page IL-3R alpha/CD123 [Unconjugated]](http://images.novusbio.com/fullsize/protein/IL-3_R_alpha_301-R3_578.jpg)
![SDS-Page IL-3R alpha/CD123 [Unconjugated]](http://images.novusbio.com/fullsize/11140-r3_recombinant-human-il-3r-alpha-cd123-fc-chimera-protein-cf-sds-page-17112022105343.jpg)
![Surface Plasmon Resonance (SPR) IL-3R alpha/CD123 [Unconjugated]](http://images.novusbio.com/images/11140-r3_recombinant-human-il-3r-alpha-cd123-fc-chimera-protein-cf-surface-plasmon-resonance-spr-2492025142019.png)
![SDS-Page IL-3R alpha/CD123 [Unconjugated]](http://images.novusbio.com/fullsize/protein/10597-r3_recombinant-cynomolgus-monkey-il-3r-alpha-fc-protein-cf-sds-page-492020115517.jpg)
