Siglec-3/CD33 Antibody (44M12D3)

Images

 
Western Blot: Siglec-3/CD33 Antibody (44M12D3) [NBP2-22377] - Analysis of CD33 in A) a partial recombinant protein and B) HCT-116 lysate using CD33 antibody at 2 ug/ml. goat anti-mouse Ig HRP secondary antibody and ...read more
Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody (44M12D3) [NBP2-22377] - Human lung tissue stained with CD33 antibody (3 ug/ml), peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
44M12D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Siglec-3/CD33 Antibody (44M12D3) Summary

Immunogen
Amino acids 42-159 of human CD33 were used as the immunogen for the antibody.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD33
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 3-6 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Publications
Read Publication using
NBP2-22377 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Siglec-3/CD33 Antibody (44M12D3)

  • CD33 antigen (gp67)
  • CD33 antigen
  • CD33 molecule
  • CD33
  • FLJ00391
  • gp67
  • myeloid cell surface antigen CD33
  • p67
  • sialic acid binding Ig-like lectin 3
  • Sialic acid-binding Ig-like lectin 3
  • Siglec3
  • Siglec-3
  • SIGLEC3gp67

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Siglec-3/CD33 Antibody (NBP2-22377)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
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Filter By Species
Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22377AF350
Alexa Fluor 405 NBP2-22377AF405
Alexa Fluor 488 NBP2-22377AF488
Alexa Fluor 532 NBP2-22377AF532
Alexa Fluor 594 NBP2-22377AF594
Alexa Fluor 647 NBP2-22377AF647
Alexa Fluor 700 NBP2-22377AF700
Alexa Fluor 750 NBP2-22377AF750
Biotin NBP2-22377B
DyLight 350 NBP2-22377UV
DyLight 405 NBP2-22377V
DyLight 488 NBP2-22377G
DyLight 550 NBP2-22377R
DyLight 594 NBP2-22377DL594
DyLight 650 NBP2-22377C
DyLight 680 NBP2-22377FR
DyLight 755 NBP2-22377IR
FITC NBP2-22377F
HRP NBP2-22377H
Janelia Fluor 549 NBP2-22377JF549
Janelia Fluor 646 NBP2-22377JF646

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Bioinformatics Tool for Siglec-3/CD33 Antibody (NBP2-22377)

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Bioinformatics

Gene Symbol CD33
Uniprot