CD83 Antibody

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CD83 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse CD83 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1437) for 3 hours at room temperature. Cells were stained with the ...read more
CD83 was detected in immersion fixed LPS-stimulated mouse dendritic cells using Goat Anti-Mouse CD83 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1437) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

CD83 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD83
Met22-Ala134
Accession # O88324
Specificity
Detects mouse CD83 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD83
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Adhesion Blockade
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF1437 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD83 Antibody

  • B-cell activation protein
  • BL11
  • BL11CD83 antigen
  • CD83 antigen (activated B lymphocytes, immunoglobulin superfamily)
  • CD83 molecule
  • CD83
  • Cell surface protein HB15
  • cell-surface glycoprotein
  • HB15
  • HB15hCD83

Background

Mouse CD83 is a 30‑35 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 114 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and a 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4). Relative to mouse, human CD83 has an 11 aa insertion in its extracellular domain and is expressed as a 45‑55 kDa protein (1, 4, 5, 6). No alternate splice variants have been reported for mouse. In human, however, one soluble splice form has been reported and proteolytic processing is suggested to generate a second circulating isoform (6, 7). Notably, although soluble CD83 has the potential to exist as either a monomer or disulfide-linked dimer, both show immunosuppressive activity (4, 8, 9). Membrane CD83, by contrast, is immunostimulatory (10). CD83 is a primary marker for dendritic cells (3, 5, 6). It is also found on B cells (6, 11), neutrophils (12), monocytes and macrophages (13). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on monocytes (3). The function of CD83 is only now becoming clear. As noted, membrane-immobilized CD83 appears to promote T cell proliferation, particularly of CD8+ cytotoxic T cells (14). On monocytes, CD83 may also drive monocytes into a fibrocyte phenotype (14). And a lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4+ T cell phenotype (15).

  1. Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
  2. Fujimoto, Y. and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
  3. Scholler, N. et. al. (2001) J. Immunol. 166:3865.
  4. Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
  5. Zhou, L-J. et. al. (1992) J. Immunol. 149:735.
  6. Hock, B.D. et al. (2001) Int. Immunol. 13:959.
  7. Dudziak, D. et al. (2005) J. Immunol. 174:6672.
  8. Kotzor, N. et al. (2004) Immunobiology 209:129.
  9. Zinser, E. et al. (2006) Immunobiology 21:449.
  10. Hirano, N. et al. (2006) Blood 107:1528.
  11. Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
  12. Yamashiro, S. et al. (2000) Blood 96:3958.
  13. Cao, W. et al. (2005) Biochem. J. 385:85.
  14. Scholler, N. et al. (2002) J. Immunol. 168:2599.
  15. Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD83 Antibody (AF1437)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ELISA Development (Capture), Flow Cytometry.


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ELISA Development (Capture)
(1)
Flow Cytometry
(1)
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Mouse
(2)
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Bioinformatics

Gene Symbol CD83
Uniprot