IL-3R alpha/CD123 Antibody (6H6) [PerCP/Cy5.5]

Flow Cytometry: IL-3 R alpha/CD123 Antibody (6H6) [PerCP/Cy5.5] [NBP2-00468] - Staining of normal human peripheral blood cells with Anti-Human CD85g (ILT7) Alexa Fluor 647 and Mouse IgG1 kappa Isotype Control PerCP-Cy5.5 (NBP1-42839) (left) or Anti-Human CD123 PerCP-Cy5.5 (right). Cells in the monocyte gate were used for analysis.

• Reactivity: Hu
• Applications: Flow
• Clone: 6H6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PerCP/Cy5.5
• Concentration: 5 mg/ml

IL-3R alpha/CD123 Antibody (6H6) [PerCP/Cy5.5] Summary

• Immunogen: Tissue / cell preparation. IL3R transfected COS cells.
• Localization: Type I membrane protein.
• Specificity: IL3RA (6H6)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL3RA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
• Application Notes: Flow Cytometry Use 1ug for 10^6 cells in 100 ul volume or 100 ul of whole blood. This antibody has been tested by tested by immunofluorescent staining with flow cytometric analysis. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Detects a band of approximately 70 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Although not tested this antibody may be useful in IHC-P and Immunoprecipitation.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (pH 7.2) with 0.1% gelatin and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 5 mg/ml
• Purity: Protein A or G purified

Alternate Names for IL-3R alpha/CD123 Antibody (6H6) [PerCP/Cy5.5]

• CD123 antigen
• CD123
• hIL3Ra
• hIL-3Ra
• IL-3 R alpha
• IL-3 receptor subunit alpha
• IL3R alpha
• IL-3R alpha
• IL-3R subunit alpha
• IL3R
• IL3RA
• IL-3Ra
• IL-3R-alpha
• IL3RAY
• IL3RX
• IL3RY
• interleukin 3 receptor, alpha (low affinity)
• interleukin-3 receptor subunit alpha
• MGC34174

Background

The 6H6 monoclonal antibody reacts with human CD123, the a chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL3RA