DLEC/CLEC4C/BDCA-2 Antibody [Unconjugated]

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Human whole blood were stained with Goat Anti- Human DLEC/CLEC4C/BDCA‑2 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF1376) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody ...read more
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human IL‑3 R alpha /CD123 APC‑conjugated Monoclonal Antibody (Catalog # FAB301A) and either (A) Goat Anti-Human DLEC/CLEC4C/BDCA‑2 ...read more
DLEC/CLEC4C/BDCA‑2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human DLEC/CLEC4C/BDCA‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1376) at 3 ...read more
DLEC/CLEC4C/BDCA-2 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human DLEC/CLEC4C/BDCA-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1376) at 15 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

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DLEC/CLEC4C/BDCA-2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human DLEC/CLEC4C/BDCA‑2
Phe46-Ile213
Accession # Q8WTT0
Specificity
Detects human DLEC/CLEC4C/BDCA‑2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CLEC4C
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF1376 in the following applications:

Publications
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AF1376 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DLEC/CLEC4C/BDCA-2 Antibody [Unconjugated]

  • BDCA2
  • BDCA-2
  • BDCA2MGC125791
  • blood dendritic cell antigen 2 protein
  • Blood dendritic cell antigen 2
  • CD303 antigen
  • CD303
  • CLEC4C
  • CLECSF11
  • CLECSF11PRO34150
  • CLECSF7
  • C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 11
  • C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 7
  • C-type lectin domain family 4 member C
  • C-type lectin superfamily member 7
  • dendritic cell lectin b
  • Dendritic lectin
  • DLEC
  • DLECMGC125792
  • HECL
  • HECLMGC125793
  • MGC125789
  • PRO34150

Background

Dendritic cell lectin (DLEC), also known as BDCA-2, CD303, HECL, and CLEC4C/CLECSF11/CLECSF7, is a 38 kDa type II transmembrane protein in the C-type lectin family (1). Mature human DLEC consists of a 21 amino acid (aa) cytoplasmic domain, a 23 aa transmembrane segment, and a 169 aa extracellular domain (ECD) that contains a juxtamembrane neck region and one carbohydrate recognition domain (CRD) (2, 3). Alternate splicing may generate multiple isoforms that lack the transmembrane segment and/or portions of the cytoplasmic, neck, and CRD regions (2-4). An ortholog of human DLEC has not been described in mouse or rat. DLEC expression is restricted to plasmacytoid dendritic cells (pDC) and is downregulated during their maturation (2, 3, 5). pDC play a role in the innate immune response by producing IFN-alpha / beta following exposure to TLR7 and TLR9 agonists such as microbial CpG DNA (3, 5-8). Antibody ligation of DLEC on pDC attenuates the CpG-stimulated production of interferons as well as a Th1 biased response (3, 5-9). DLEC interactions with HIV-1 gp120 and hepatitis B virus soluble antigen may therefore limit the pDC antiviral response (10, 11). Similar to other C-type lectins, DLEC can mediate antigen uptake for MHC loading and presentation to T cells (3, 12). Crosslinking of DLEC on CpG-stimulated pDC inhibits pDC maturation and induces tyrosine phosphorylation on multiple proteins involved in B cell receptor signaling and endocytosis (5, 7, 8). These functions require the association of DLEC with the ITAM-containing Fc epsilon RI gamma chain (7, 8).
  1. Kanazawa, N. et al. (2004) Immunobiology 209:179.
  2. Arce, I. et al. (2001) Eur. J. Immunol. 31:2733.
  3. Dzionek, A. et al. (2001) J. Exp. Med. 194:1823.
  4. Fernandes, M.J. et al. (2000) Genomics 69:263.
  5. Wu, P. et al. (2008) Clin. Immunol. 129:40.
  6. Fanning, S.L. et al. (2006) J. Immunol. 177:5829.
  7. Rock, J. et al. (2007) Eur. J. Immunol. 37:3564.
  8. Cao, W. et al. (2007) PloS Biol. 5:e248.
  9. Riboldi, E. et al. (2009) Immunobiology 214:868.
  10. Martinelli, E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:3396.
  11. Xu, Y. et al. (2009) Mol. Immunol. 46:2640.
  12. Jaehn, P.S. et al. (2008) Eur. J. Immunol. 38:1822.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DLEC/CLEC4C/BDCA-2 Antibody (AF1376)(3)

Review for DLEC/CLEC4C/BDCA-2 Antibody (AF1376) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1376:
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Immunohistochemistry-Paraffin
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin DLEC/CLEC4C/BDCA-2 AF1376
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4
reviewed by:
Verified Customer
Immunohistochemistry-Paraffin Human 05/17/2018
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman lymph node
SpeciesHuman
LotIDL0217051

Comments

CommentsHeat mediated antigen retrieval performed in citrate buffer for 20 minutes (pH 6.0)

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Verified Customer
05/17/2018
Application: Immunohistochemistry-Paraffin
Species: Human

Bioinformatics

Gene Symbol CLEC4C
Uniprot