IL-23A/IL-23 P19 Antibody - BSA Free

Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Increased cytokine expression in N-WASP-null skin. Representative immunoblots and quantification of indicated proteins from epidermal lysates of 7-8-week old con and ko mice (n: 4/4, mean?+-?SD, two-tailed unpaired t-test). Representative immunoblots and quantification of indicated proteins of co-precipitations of IL-23p19 with IL-12p40 from epidermal lysates of 7-8-week-old con and ko mice (n: 4/4, mean?+-?SD, two-tailed unpaired t-test; *p?less than or equal ?0.05; **p?less than or equal ?0.01; ***p?less than or equal ?0.001) Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03704-z) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Abdominal Aortic Aneurysm (AAA) Tissues (Validation of IL-23 in Human Abdominal Aortic Aneurysm (AAA) Tissues

Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Western blot analysis of IL-23 in Raji lysate with IL-23 antibody at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.

Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Loading: 30 ng of human IL-23 recombinant protein.Antibodies: IL-23 (4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Loading: 20ug of lysates per lane.Antibodies: IL-23, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry: IL-23A/IL-23 P19 Antibody [NBP1-77257] - Staining of human pancreas tissue with antibody at 5 ug/mL.

Western Blot: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Increased cytokine expression in N-WASP-null skin. a qRT-PCR analysis of indicated genes from back skin RNA of con & ko at indicated ages (n > = 3/3, two-tailed unpaired t-test). All normalized to GAPDH. b Representative toluidine blue staining for barrier defect in con & ko mice at indicated ages (P: E15.5 embryos used as positive controls; 2 days (2d) n: 6/5, 6 days(6d) n: 4/5, 3 independent experiments). c Representative immunofluorescence staining & quantification of IL-23p19 of con & ko back skin at indicated ages (n: 3/3, two-tailed paired t-test). Scale bar: 100 µm. d Representative sorting strategy of CD45+, alpha 6H, & alpha 6L cells from con & ko epidermal cells & qRT-PCR analysis of IL-23A & IL-17A expression in sorted cells (n: 3/3, mean ± SD, two-tailed unpaired t-test, 3 independent sortings). e Representative immunoblots & quantification of indicated proteins from epidermal lysates of 7–8-week old con & ko mice (n: 4/4, mean ± SD, two-tailed unpaired t-test). f Representative immunoblots & quantification of indicated proteins of co-precipitations of IL-23p19 with IL-12p40 from epidermal lysates of 7–8-week-old con & ko mice (n: 4/4, mean ± SD, two-tailed unpaired t-test; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001) Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03704-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Raji Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Raji Cells labeling IL-23A/IL-23 P19 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Raji Cells. Immunocytochemical analysis of Raji cells using anti-IL-23A/IL-23 P19 antibody at 10 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation of IL-23A/IL-23 P19 in Human Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human pancreas tissue labeling IL-23A/IL-23 P19 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Western Blot: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-77257] - Validation in Human Raji Cells. Loading: 15 ug of lysates per lane.Antibodies: IL-23A/IL-23 P19 (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane A: Absence of blocking peptideLane B: Presence of blocking peptide.

• Reactivity: Hu, Mu, Eq, Gp
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IL-23A/IL-23 P19 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IL-23A/IL-23 P19 Antibody - BSA Free (NBP1-77257) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. Anti-IL-23A/IL-23 P19 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of mature human IL-23. The immunogen is located the last 50 amino acids of IL-23.
• Predicted Species: Guinea Pig (93%), Equine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL23A
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/mL
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation
  • Western Blot 1-4 ug/ml
• Application Notes: Use in immunoprecipitation reported in scientific literature (PMID: 29650963).
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IL-23A/IL-23 P19 Peptide (NBP1-77257PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IL-23A/IL-23 P19 Antibody - BSA Free

• IL-23 p19/IL-12 p40
• IL23
• IL-23
• IL-23A
• IL-23-A
• IL-23p19
• IL-23p19/IL-12p40
• IL23P19P19
• interleukin 23 p19 subunit
• interleukin 23, alpha subunit p19
• interleukin-23 subunit alpha
• Interleukin-23 subunit p19
• JKA3 induced upon T-cell activation
• MGC79388
• SGRF
• SGRFIL-23 subunit alpha

Background

Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-gamma, IL-12, and TNF-alpha from activated T cells, and can stimulate macrophages to produce TNF-alpha and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-23A/IL-23 P19 Antibody (NBP1-77257)(2)

Publications using NBP1-77257

• Reiszadeh-Jahromi S, Haddadi M, Mousavi P, Sanadgol N Prophylactic effects of cucurbitacin B in the EAE Model of multiple sclerosis by adjustment of STAT3/IL-23/IL-17 axis and improvement of neuropsychological symptoms Metabolic brain disease 2022-10-26 [PMID: 36287356]
• Li H, Yao Q, Mariscal AG et al. Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation. Nat Commun 2018-04-12 [PMID: 29650963] (IP, Human)

Bioinformatics

• Gene Symbol: IL23A
• Uniprot:
  • AAH66268(Human)