Recombinant Human IL-27 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-27 Protein Summary

Details of Functionality
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al. (2009) Hepatology 50:585. The ED50 for this effect is 1-8 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-27 protein
Human IL-27 EBI-3
(Arg21-Lys229)
Accession # Q14213.2
GSGSSRGGSGSGGSGGGGSKL  Human IL-27 p28
(Phe29-Pro243)
Accession # AAM34498
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Arg21
Protein/Peptide Type
Recombinant Proteins
Gene
IL27
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60 kDa, reducing conditions
Publications
Read Publications using
2526-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, EDTA and CHAPS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-27 Protein

  • IL-27 p28 subunit
  • IL-27 subunit alpha
  • IL27
  • IL-27
  • IL27A
  • IL-27-A
  • IL27-A
  • IL27p28
  • IL30
  • interleukin 27
  • interleukin 30
  • interleukin-27 subunit alpha
  • MGC71873
  • p28IL-27A

Background

IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2-4). The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein (5). The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family (5). Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region (6). The mature region is characterized by the presence of four alpha -helices, placing it in the IL-6 family of helical cytokines. Human p28 is 74% aa identical to mouse p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells (7). IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1 (6, 8, 9). IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity (3, 7). At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development) (10).

  1. Boulay, J-L. et al. (2003) Immunity 19:159. 
  2. Trinchieri, G. et al. (2003) Immunity 19:641. 
  3. Murakami, M. et al. (2004) Growth Factors 22:75. 
  4. Cordoba-Rodriguez, R. and D.M. Frucht (2003) Exp. Opin. Biol. Ther. 3:715. 
  5. Devergne, O. et al. (1996) J. Virology 70:1143. 
  6. Pflanz, S. et al. (2002) Immunity 16:779.
  7. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
  8. Pflanz, S. et al. (2004) J Immunol 172:2225.
  9. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
  10. Holscher, C. (2004) Med. Microbiol. Immunol. (Berl).193:1.

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2526-IL
Species: Hu
Applications: Bioactivity

Publications for IL-27 (2526-IL)(30)

We have publications tested in 2 confirmed species: Human, Complex Species Category.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.


Filter By Application
Bioassay
(27)
Cell Culture
(1)
In Vivo
(1)
All Applications
Filter By Species
Human
(29)
Complex Species Category
(1)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using 2526-IL Applications Species
D Yamasoba, K Sato, T Ichinose, T Imamura, L Koepke, S Joas, E Reith, D Hotter, N Misawa, K Akaki, T Uehata, T Mino, S Miyamoto, T Noda, A Yamashita, DM Standley, F Kirchhoff, D Sauter, Y Koyanagi, O Takeuchi N4BP1 restricts HIV-1 and its inactivation by MALT1 promotes viral reactivation Nat Microbiol, 2019;0(0):. 2019 [PMID: 31133753] (Bioassay, Human) Bioassay Human
C Petes, V Mintsopoul, RL Finnen, BW Banfield, K Gee The effects of CD14 and IL-27 on induction of endotoxin tolerance in human monocytes and macrophages J. Biol. Chem., 2018;0(0):. 2018 [PMID: 30242126] (Human) Human
C Petes, N Odoardi, SM Plater, NL Martin, K Gee IL-27 amplifies cytokine responses to Gram-negative bacterial products and Salmonella typhimurium infection Sci Rep, 2018;8(1):13704. 2018 [PMID: 30209294] (Bioassay, Human) Bioassay Human
C Rolvering, AD Zimmer, A Ginolhac, C Margue, M Kirchmeyer, F Servais, HM Hermanns, S Hergovits, PV Nazarov, N Nicot, S Kreis, S Haan, I Behrmann, C Haan The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by ?-PD-L1 or ?-IL6 antibodies J. Leukoc. Biol., 2018;0(0):. 2018 [PMID: 30040142] (Bioassay, Human) Bioassay Human
Q Zhang, APD Cunha, S Li, Q Hao, V Kainz, Q Huang, HY Wu IL-27 regulates HIF-1?-mediated VEGFA response in macrophages of diabetic retinopathy patients and healthy individuals Cytokine, 2018;0(0):. 2018 [PMID: 30007476] (Bioassay, Human) Bioassay Human
MV Patel, Z Shen, RM Rossoll, CR Wira IL-27 Expression and Responsiveness in Human Uterine Epithelial Cells and Fibroblasts In Vitro and the Role of Estradiol J. Interferon Cytokine Res., 2018;38(3):101-110. 2018 [PMID: 29565744] (Bioassay, Human) Bioassay Human
I Gregersen, Ø Sandanger, ET Askevold, EL Sagen, K Yang, S Holm, TM Pedersen, M Skjelland, K Krohg-Søre, TV Hansen, TB Dahl, K Otterdal, T Espevik, P Aukrust, A Yndestad, B Halvorsen Interleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activation PLoS ONE, 2017;12(11):e0188387. 2017 [PMID: 29176764] (Bioassay, Human) Bioassay Human
Yuan Zhang, Chi A Ma, Monica G Lawrence, Timothy J Break, Michael P O'connell, Jonathan J Lyons, Diego B López, John S Barber, Yongge Zhao, Daniel L Barber, Alexandra F Freeman, Steven M Holland, Michail S Lionakis, Joshua D Milner PD-L1 up-regulation restrains Th17 cell differentiation in STAT3 loss- and STAT1 gain-of-function patients. The Journal of Experimental Medicine, 2017;0(0):1540-9538. 2017 [PMID: 28710273] (Bioassay, Human) Bioassay Human
J Richard, J Prévost, B von Bredow, S Ding, N Brassard, H Medjahed, M Coutu, B Melillo, F Bibollet-R, BH Hahn, DE Kaufmann, AB Smith, J Sodroski, D Sauter, F Kirchhoff, K Gee, SJ Neil, DT Evans, A Finzi BST-2 Expression Modulates Small CD4 Mimetic Sensitization of HIV-1-infected cells to ADCC J. Virol, 2017;0(0):. 2017 [PMID: 28331088] (Bioassay, Human) Bioassay Human
KO Dixon, J O'Flynn, N Klar-Moham, MR Daha, C van Kooten Properdin and factor H production by human dendritic cells modulates their T-cell stimulatory capacity and is regulated by IFN-? Eur. J. Immunol, 2017;0(0):. 2017 [PMID: 28105653] (Bioassay, Human) Bioassay Human
Show All 30 Publications.

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