Recombinant Human IL-27 Protein


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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-27 Protein Summary

Details of Functionality
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al. (2009) Hepatology 50:585. The ED50 for this effect is 1-8 ng/mL.
Mouse myeloma cell line, NS0-derived human IL-27 protein
Human IL-27 EBI-3
Accession # Q14213.2
Accession # AAM34498
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
60 kDa, reducing conditions
Read Publications using
2526-IL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, EDTA and CHAPS with BSA as a carrier protein.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-27 Protein

  • IL-27 p28 subunit
  • IL-27 subunit alpha
  • IL27
  • IL-27
  • IL27A
  • IL-27-A
  • IL27-A
  • IL27p28
  • IL30
  • interleukin 27
  • interleukin 30
  • interleukin-27 subunit alpha
  • MGC71873
  • p28IL-27A


IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2-4). The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein (5). The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family (5). Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region (6). The mature region is characterized by the presence of four alpha -helices, placing it in the IL-6 family of helical cytokines. Human p28 is 74% aa identical to mouse p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells (7). IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1 (6, 8, 9). IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity (3, 7). At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development) (10).

  1. Boulay, J-L. et al. (2003) Immunity 19:159. 
  2. Trinchieri, G. et al. (2003) Immunity 19:641. 
  3. Murakami, M. et al. (2004) Growth Factors 22:75. 
  4. Cordoba-Rodriguez, R. and D.M. Frucht (2003) Exp. Opin. Biol. Ther. 3:715. 
  5. Devergne, O. et al. (1996) J. Virology 70:1143. 
  6. Pflanz, S. et al. (2002) Immunity 16:779.
  7. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
  8. Pflanz, S. et al. (2004) J Immunol 172:2225.
  9. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
  10. Holscher, C. (2004) Med. Microbiol. Immunol. (Berl).193:1.

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Publications for IL-27 (2526-IL)(30)

We have publications tested in 2 confirmed species: Human, Complex Species Category.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.

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Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using 2526-IL Applications Species
D Yamasoba, K Sato, T Ichinose, T Imamura, L Koepke, S Joas, E Reith, D Hotter, N Misawa, K Akaki, T Uehata, T Mino, S Miyamoto, T Noda, A Yamashita, DM Standley, F Kirchhoff, D Sauter, Y Koyanagi, O Takeuchi N4BP1 restricts HIV-1 and its inactivation by MALT1 promotes viral reactivation Nat Microbiol, 2019;0(0):. 2019 [PMID: 31133753] (Bioassay, Human) Bioassay Human
C Petes, V Mintsopoul, RL Finnen, BW Banfield, K Gee The effects of CD14 and IL-27 on induction of endotoxin tolerance in human monocytes and macrophages J. Biol. Chem., 2018;0(0):. 2018 [PMID: 30242126] (Human) Human
C Petes, N Odoardi, SM Plater, NL Martin, K Gee IL-27 amplifies cytokine responses to Gram-negative bacterial products and Salmonella typhimurium infection Sci Rep, 2018;8(1):13704. 2018 [PMID: 30209294] (Bioassay, Human) Bioassay Human
C Rolvering, AD Zimmer, A Ginolhac, C Margue, M Kirchmeyer, F Servais, HM Hermanns, S Hergovits, PV Nazarov, N Nicot, S Kreis, S Haan, I Behrmann, C Haan The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by ?-PD-L1 or ?-IL6 antibodies J. Leukoc. Biol., 2018;0(0):. 2018 [PMID: 30040142] (Bioassay, Human) Bioassay Human
Q Zhang, APD Cunha, S Li, Q Hao, V Kainz, Q Huang, HY Wu IL-27 regulates HIF-1?-mediated VEGFA response in macrophages of diabetic retinopathy patients and healthy individuals Cytokine, 2018;0(0):. 2018 [PMID: 30007476] (Bioassay, Human) Bioassay Human
MV Patel, Z Shen, RM Rossoll, CR Wira IL-27 Expression and Responsiveness in Human Uterine Epithelial Cells and Fibroblasts In Vitro and the Role of Estradiol J. Interferon Cytokine Res., 2018;38(3):101-110. 2018 [PMID: 29565744] (Bioassay, Human) Bioassay Human
I Gregersen, Ø Sandanger, ET Askevold, EL Sagen, K Yang, S Holm, TM Pedersen, M Skjelland, K Krohg-Søre, TV Hansen, TB Dahl, K Otterdal, T Espevik, P Aukrust, A Yndestad, B Halvorsen Interleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activation PLoS ONE, 2017;12(11):e0188387. 2017 [PMID: 29176764] (Bioassay, Human) Bioassay Human
Yuan Zhang, Chi A Ma, Monica G Lawrence, Timothy J Break, Michael P O'connell, Jonathan J Lyons, Diego B López, John S Barber, Yongge Zhao, Daniel L Barber, Alexandra F Freeman, Steven M Holland, Michail S Lionakis, Joshua D Milner PD-L1 up-regulation restrains Th17 cell differentiation in STAT3 loss- and STAT1 gain-of-function patients. The Journal of Experimental Medicine, 2017;0(0):1540-9538. 2017 [PMID: 28710273] (Bioassay, Human) Bioassay Human
J Richard, J Prévost, B von Bredow, S Ding, N Brassard, H Medjahed, M Coutu, B Melillo, F Bibollet-R, BH Hahn, DE Kaufmann, AB Smith, J Sodroski, D Sauter, F Kirchhoff, K Gee, SJ Neil, DT Evans, A Finzi BST-2 Expression Modulates Small CD4 Mimetic Sensitization of HIV-1-infected cells to ADCC J. Virol, 2017;0(0):. 2017 [PMID: 28331088] (Bioassay, Human) Bioassay Human
KO Dixon, J O'Flynn, N Klar-Moham, MR Daha, C van Kooten Properdin and factor H production by human dendritic cells modulates their T-cell stimulatory capacity and is regulated by IFN-? Eur. J. Immunol, 2017;0(0):. 2017 [PMID: 28105653] (Bioassay, Human) Bioassay Human
Show All 30 Publications.

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