IL-17D Antibody (312724) [Unconjugated]

Mouse splenocytes were stained with Rat Anti-Mouse IL‑17D Monoclonal Antibody (Catalog # MAB2274, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with para­formaldehyde and permeabilized with saponin.

• Reactivity: Mu
• Applications: WB, CyTOF-ready, Flow
• Clone: 312724
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-17D Antibody (312724) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL‑17D
  Ala25-Arg205
  Accession # NP_665836
• Specificity: Detects mouse IL-17D in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human IL-17D, recombinant mouse (rm) IL-17, rmIL-17B, rmIL-17C, rmIL-17E, or rmIL-17F is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL17D
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17D Antibody (312724) [Unconjugated]

• FLJ30846
• IL17D
• IL-17D
• IL-17Dinterleukin 27
• IL-22
• IL-27interleukin-17D
• IL27interleukin-27
• interleukin 17D
• Interleukin-27

Background

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus (1, 2, 6). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2, 6). Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members, have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have also been reported (1‑6). Mouse IL-17D is synthesized as a 205 amino acid (aa) precursor that contains a putative 24 aa signal peptide and a 181 aa mature segment. The mature region contains two potential N-linked glycosylation sites and eight cysteines, four of which are involved in the formation of a modified cysteine-knot motif (5). The molecule is reported to exist as a 53 kDa disulfide-linked homodimer (2, 5). Given that its predicted homodimeric molecular weight is 40 kDa, the molecule is presumably glycosylated. In the mature region, mouse IL-17D is 88% aa identical to human IL-17D. There is less than 30% aa identity between mouse IL-17D and other members of the mouse IL-17 family. IL-17D is expressed in skeletal muscle, adipose tissue, fetal liver, and heart, plus resting CD4⁺ T cells and CD19⁺ B cells (1). R&D Systems has shown IL-17D binding to a mouse IL-17 R/Fc construct in a functional ELISA. IL-17D is known to induce the production of IL-8, IL-6 and GM-CSF (5).

1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
3. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
4. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
5. Starnes, T. et al. (2002) J. Immunol. 169:642.
6. Kolls, J.K. and A. Linden (2004) Immunity 21:467.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17D Antibody (MAB2274)(1)

Publication using MAB2274

• R Seelige, R Saddawi-Ko, NM Adams, G Picarda, JC Sun, CA Benedict, JD Bui Interleukin-17D and Nrf2 mediate initial innate immune cell recruitment and restrict MCMV infection Sci Rep, 2018-09-12;8(1):13670. 2018-09-12 [PMID: 30209334] (Mouse)

Bioinformatics

• Gene Symbol: IL17D
• Uniprot:
  • NP_665836()