RPSA Antibody (RPSA/2699) - Azide and BSA Free

Images

 
Western Blot: RPSA Antibody (RPSA/2699) - Azide and BSA Free [NBP2-79854] - Western Blot Analysis of human THP-1, U937, SK-BR3, and A431 cell lysates. RPSA Antibody (RPSA/2699).
Immunocytochemistry/ Immunofluorescence: RPSA Antibody (RPSA/2699) - Azide and BSA Free [NBP2-79854] - Immunofluorescent staining of paraformaldehyde-Raji cells using RPSA Antibody (RPSA/2699) followed by goat ...read more
Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) - Azide and BSA Free [NBP2-79854] - Formalin-fixed, paraffin-embedded human Tonsil stained with RPSA Antibody (RPSA/2699).
Flow Cytometry: RPSA Antibody (RPSA/2699) - Azide and BSA Free [NBP2-79854] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG-CF488 (blue) and ...read more
Immunocytochemistry/ Immunofluorescence: RPSA Antibody (RPSA/2699) - Azide and BSA Free [NBP2-79854] - Immunofluorescent staining of HeLa cells using RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated ...read more
Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) - Azide and BSA Free [NBP2-79854] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with RPSA Antibody (RPSA/2699).
Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) - Azide and BSA Free [NBP2-79854] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with RPSA Antibody (RPSA/2699).
Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) - Azide and BSA Free [NBP2-79854] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with RPSA Antibody (RPSA/2699).
Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) - Azide and BSA Free [NBP2-79854] - Formalin-fixed, paraffin-embedded human Cervix Carcinoma stained with RPSA Antibody (RPSA/2699).
Flow Cytometry: RPSA Antibody (RPSA/2699) - Azide and BSA Free [NBP2-79854] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG-CF488 (blue) and ...read more
Protein Array: RPSA Antibody (RPSA/2699) - Azide and BSA Free [NBP2-79854] - Analysis of Protein Array containing more than 19,000 full-length human proteins using RPSA Antibody (RPSA/2699) Z- and S- Score: The Z-score ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, MA, CyTOF-ready
Clone
RPSA/2699
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

RPSA Antibody (RPSA/2699) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79720).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
Immunogen
Recombinant full-length human RPSA protein (Uniprot: P08865)
Localization
Cell Surface, Cytoplasmic, Nuclear
Marker
Marker of Metastatic Potential
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
RPSA
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1-2 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for RPSA Antibody (RPSA/2699) - Azide and BSA Free

  • 37 kDa laminin receptor precursor
  • 37/67 kDa laminin receptor
  • 37LRPNEM/1CHD4
  • 67 kDa laminin receptor
  • 67kD, ribosomal protein SA
  • 67LR
  • Colon carcinoma laminin-binding protein
  • LAMBR37 kDa laminin receptor
  • laminin binding protein
  • laminin receptor 1 (67kD, ribosomal protein SA)
  • Laminin receptor 1
  • Laminin-binding protein precursor p40
  • LAMR 1
  • lamR
  • LAMR140S ribosomal protein SA
  • LBP
  • LBP/p40
  • LRP
  • LRP/LR
  • Multidrug resistance-associated protein MGr1-Ag
  • p40
  • ribosomal protein SA

Background

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol RPSA