IL-23A/IL-23 P19 Antibodies

402-ML), Recombinant Mouse IL-23 (Catalog # 1887-ML) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Mouse IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1619). The ND₅₀ is typically 0.07-0.35 µg/mL." class="big_lightbox"> 402-ML), Recombinant Mouse IL-23 (Catalog # 1887-ML) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Mouse IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1619). The ND₅₀ is typically 0.07-0.35 µg/mL." alt="In the presence of Recombinant Mouse IL-2 (10 ng/mL, Catalog # 402-ML), Recombinant Mouse IL-23 (Catalog # 1887-ML) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Mouse IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1619). The ND₅₀ is typically 0.07-0.35 µg/mL." class="big_thumb" />

402-ML), Recombinant Human IL-23 (Catalog # 1290-IL) stim-ulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1716). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_lightbox"> 402-ML), Recombinant Human IL-23 (Catalog # 1290-IL) stim-ulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1716). The ND₅₀ is typically 0.2-0.8 µg/mL." alt="In the presence of Recombinant Mouse IL-2 (10 ng/mL, Catalog # 402-ML), Recombinant Human IL-23 (Catalog # 1290-IL) stim-ulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1716). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_thumb" />

HAF109). A specific band was detected for IL-23 p19 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF109). A specific band was detected for IL-23 p19 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF007). A specific band was detected for IL-23 p19 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). A specific band was detected for IL-23 p19 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of CHO Chinese hamster ovary cell line transfected with human IL-23. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-23 p19 Monoclonal Antibody (Catalog # MAB17161) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-23 p19 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

DY990). Mouse Anti-Human EBI3 Monoclonal Antibody (MAB64561) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Mouse Anti-Human EBI3 Monoclonal Antibody (MAB64561) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

HAF008). A specific band was detected for IL-23 p19 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for IL-23 p19 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with human IL-23 p19. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human IL-23 p19 Monoclonal Antibody (Catalog # MAB12901) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IL-23 p19 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

1290-IL) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.3 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-23 p19 Monoclonal Antibody (Catalog # MAB1290). The ND₅₀ is typically 0.15-0.9 µg/mL." class="big_lightbox"> 1290-IL) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.3 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-23 p19 Monoclonal Antibody (Catalog # MAB1290). The ND₅₀ is typically 0.15-0.9 µg/mL." class="big_thumb" />

HAF016). A specific band was detected for IL-23 p19 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF016). A specific band was detected for IL-23 p19 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of Hs 294T human melanoma cell line. PVDF Membrane was probed with 1 µg/mL of Feline IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6238) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-23 p19 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

HAF016). A specific band was detected for IL-23 p19 at approximately 26-28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF016). A specific band was detected for IL-23 p19 at approximately 26-28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of human mature dendritic cells (LPS activated), CLL-1390 canine leukocytic round cell neoplasia cell line, and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Canine IL-23 p19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6250) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-23 p19 at approximately 26-28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />