p63/TP73L Antibody [Unconjugated]

p63/TP73L was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

p63/TP73L Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human p63/TP73L
  Met40-Cys339
  Accession # Q9H3D4
• Specificity: Detects human p63/TP73L in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TP63
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
• Control:

  p63/TP73L Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p63/TP73L Antibody [Unconjugated]

• amplified in squamous cell carcinoma
• Chronic ulcerative stomatitis protein
• CUSP
• EEC3
• EEC3B(p51B)
• Keratinocyte transcription factor KET
• KET
• KETRHS
• LMS
• OFC8
• OFC8SHFM4TP73LNBP
• p40
• p51
• p51tumor protein p53-like
• p53CP
• p63
• p63AIS
• p73H
• p73L
• p73LB(p51A)
• SHFM4
• TP53CP
• TP53L
• TP63
• TP73L
• Transformation-related protein 63
• tumor protein 63
• tumor protein p53-competing protein
• tumor protein p63 deltaN isoform delta
• tumor protein p63
• Tumor protein p73-like

Background

Tumor Protein 63 (p63), also named TP73L, TP63, p51, p40 or KET, is a p53 homolog. It is one of several proteins that are produced from a single gene using two promoters and alternative splicing of the primary RNA transcript. p63 is highly expressed in embryonic ectoderm and in the nuclei of basal regenerative cells of many epithelial tissues in the adult. p63 is suggested to play a role in development, epithelial cell maintenance and tumorigenesis (1‑3).

1. Harms, K. et al. (2004), Cell Mol. Life Sci. 61(7):822.
2. Koster, M.I. and D.R. Roop, J. Dermatol. Sci. 34(1):3.
3. Benard, J. et al. (2003) Hum. Mutat. 21(3):182.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p63/TP73L Antibody (AF1916)(3)

Publications using AF1916

• Y Liu, N Yin, X Wang, A Khoor, V Sambandam, AB Ghosh, ZA Fields, NR Murray, V Justilien, AP Fields Chromosome 3q26 Gain Is an Early Event Driving Coordinated Overexpression of the PRKCI, SOX2, and ECT2 Oncogenes in Lung Squamous Cell Carcinoma Cell Rep, 2020;30(3):771-782.e6. 2020 [PMID: 31968252] (IHC, Mouse)
• B You, F Mercier, E Assenat, C Langlois-J, O Glehen, J Soulé, L Payen, V Kepenekian, M Dupuy, F Belouin, E Morency, V Saywell, M Flacelière, P Elies, P Liaud, T Mazard, D Maucort-Bo, W Tan, B Vire, L Villeneuve, M Ychou, M Kohli, D Joubert, A Prieur The oncogenic and druggable hPG80 (Progastrin) is overexpressed in multiple cancers and detected in the blood of patients EBioMedicine, 2019;51(0):102574. 2019 [PMID: 31877416] (IHC, Human)
• R Belgacemi, E Luczka, J Ancel, Z Diabasana, JM Perotin, A Germain, N Lalun, P Birembaut, X Dubernard, JC Mérol, G Delepine, M Polette, G Deslée, V Dormoy Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD EBioMedicine, 2019;51(0):102572. 2019 [PMID: 31877414] (IHC, Human)

Review for p63/TP73L Antibody (AF1916) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Tony Reyes IHC-P Mouse 09/23/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse lung paraffin embedded sections,mouse lung tissue
• Species: Mouse
• Lot: KFX0120011

Comments

• Comments: p63/TP73L was detected in paraffin-embedded mouse tissue from Lkb1fl/fl; Ptenfl/fl and Sox2LSL/LSL ;Nkx2-1fl/fl; Lkb1fl/fl (SNL) mice, which present with lung squamous cell carcinoma (LSCC) tumors, using the Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) overnight at 4℃ in blocking buffer at a dilution of 1/400. Citrate buffer pH 6.0 was used for high temperature antigen retrieval for 15 minutes in a pressure cooker. Tissue was stained using a HRP-conjugated secondary antibody and DAB staining and counterstained with hematoxylin (blue). P63 positivity was seen in the basal cells found in the trachea of mice and in LSCC tumors but not in small cell lung cancer (SCLC) tumors from Rb1/p53/Myc and Rb1/p53/Rbl2 mice or in mucinous adenocarcinoma tumors found in SNL mice.

Bioinformatics

• Gene Symbol: TP63
• Uniprot:
  • Q9H3D4()