Home » p63/TP73L » p63/TP73L Antibodies » p63/TP73L Antibody [Unconjugated]

p63/TP73L Antibody [Unconjugated]

 (5 Publications)
 (1 Review)
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p63/TP73L was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
p63/TP73L was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 15 µg/mL overnight at 4 °C. ...read more
p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1916
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Catalog# & Formulation Size Price
Carrier Free
AF1916-SP
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p63/TP73L Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human p63/TP73L
Met40-Cys339
Accession # Q9H3D4
Specificity
Detects human p63/TP73L in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TP63
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF1916 in the following applications:
Publications
Read Publications using
AF1916 in the following applications:

  • IHC
    5 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for p63/TP73L Antibody [Unconjugated]

  • amplified in squamous cell carcinoma
  • Chronic ulcerative stomatitis protein
  • CUSP
  • EEC3
  • EEC3B(p51B)
  • Keratinocyte transcription factor KET
  • KET
  • KETRHS
  • LMS
  • OFC8
  • OFC8SHFM4TP73LNBP
  • p40
  • p51
  • p51tumor protein p53-like
  • p53CP
  • p63
  • p63AIS
  • p73H
  • p73L
  • p73LB(p51A)
  • SHFM4
  • TP53CP
  • TP53L
  • TP63
  • TP73L
  • Transformation-related protein 63
  • tumor protein 63
  • tumor protein p53-competing protein
  • tumor protein p63 deltaN isoform delta
  • tumor protein p63
  • Tumor protein p73-like

Background

Tumor Protein 63 (p63), also named TP73L, TP63, p51, p40 or KET, is a p53 homolog. It is one of several proteins that are produced from a single gene using two promoters and alternative splicing of the primary RNA transcript. p63 is highly expressed in embryonic ectoderm and in the nuclei of basal regenerative cells of many epithelial tissues in the adult. p63 is suggested to play a role in development, epithelial cell maintenance and tumorigenesis (1‑3).

  1. Harms, K. et al. (2004), Cell Mol. Life Sci. 61(7):822.
  2. Koster, M.I. and D.R. Roop, J. Dermatol. Sci. 34(1):3.
  3. Benard, J. et al. (2003) Hum. Mutat. 21(3):182.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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p63/TP73L Antibody
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p63/TP73L Antibody [Unconjugated]
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Publications for p63/TP73L Antibody (AF1916)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(5)
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(4)
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(1)
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Showing Publications 1 - 5 of 5.
Publications using AF1916 Applications Species
Z Diabasana, JM Perotin, R Belgacemi, J Ancel, P Mulette, G Delepine, P Gosset, U Maskos, M Polette, G Deslée, V Dormoy Nicotinic Receptor Subunits Atlas in the Adult Human Lung Int J Mol Sci, 2020;21(20):. 2020 [PMID: 33050277] (IHC, Human) IHC Human
J Ancel, R Belgacemi, JM Perotin, Z Diabasana, S Dury, M Dewolf, A Bonnomet, N Lalun, P Birembaut, M Polette, G Deslée, V Dormoy Sonic hedgehog signalling as a potential endobronchial biomarker in COPD Respir. Res., 2020;21(1):207. 2020 [PMID: 32767976] (IHC, Human) IHC Human
Y Liu, N Yin, X Wang, A Khoor, V Sambandam, AB Ghosh, ZA Fields, NR Murray, V Justilien, AP Fields Chromosome 3q26 Gain Is an Early Event Driving Coordinated Overexpression of the PRKCI, SOX2, and ECT2 Oncogenes in Lung Squamous Cell Carcinoma Cell Rep, 2020;30(3):771-782.e6. 2020 [PMID: 31968252] (IHC, Mouse) IHC Mouse
B You, F Mercier, E Assenat, C Langlois-J, O Glehen, J Soulé, L Payen, V Kepenekian, M Dupuy, F Belouin, E Morency, V Saywell, M Flacelière, P Elies, P Liaud, T Mazard, D Maucort-Bo, W Tan, B Vire, L Villeneuve, M Ychou, M Kohli, D Joubert, A Prieur The oncogenic and druggable hPG80 (Progastrin) is overexpressed in multiple cancers and detected in the blood of patients EBioMedicine, 2019;51(0):102574. 2019 [PMID: 31877416] (IHC, Human) IHC Human
R Belgacemi, E Luczka, J Ancel, Z Diabasana, JM Perotin, A Germain, N Lalun, P Birembaut, X Dubernard, JC Mérol, G Delepine, M Polette, G Deslée, V Dormoy Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD EBioMedicine, 2019;51(0):102572. 2019 [PMID: 31877414] (IHC, Human) IHC Human

Review for p63/TP73L Antibody (AF1916) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF1916:
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin p63/TP73L AF1916
Enlarge 		5
reviewed by:
Tony Reyes
 IHC-P Mouse 09/23/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse lung paraffin embedded sections,mouse lung tissue
SpeciesMouse
LotKFX0120011

Comments

Commentsp63/TP73L was detected in paraffin-embedded mouse tissue from Lkb1fl/fl; Ptenfl/fl and Sox2LSL/LSL ;Nkx2-1fl/fl; Lkb1fl/fl (SNL) mice, which present with lung squamous cell carcinoma (LSCC) tumors, using the Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) overnight at 4℃ in blocking buffer at a dilution of 1/400. Citrate buffer pH 6.0 was used for high temperature antigen retrieval for 15 minutes in a pressure cooker. Tissue was stained using a HRP-conjugated secondary antibody and DAB staining and counterstained with hematoxylin (blue). P63 positivity was seen in the basal cells found in the trachea of mice and in LSCC tumors but not in small cell lung cancer (SCLC) tumors from Rb1/p53/Myc and Rb1/p53/Rbl2 mice or in mucinous adenocarcinoma tumors found in SNL mice.

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Secondary Antibodies

 

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Immunohistochemistry-Paraffin p63/TP73L AF1916
Enlarge
 Tony Reyes
09/23/2020
Application: IHC-P
Species: Mouse
read this review

Bioinformatics

Gene Symbol TP63
Uniprot