GEF-H1 Antibody - BSA Free

Western Blot: GEF-H1 Antibody [NBP2-21577] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody diluted at 1:4000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: GEF-H1 Antibody [NBP2-21577] - GEF-H1 antibody detects GEF-H1 protein at cytoskeleton and Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GEF-H1 stained by GEF-H1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.

Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577] - GEF-H1 antibody detects GEF-H1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GEF-H1 stained by GEF-H1 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: GEF-H1 Antibody [NBP2-21577] - Sample (10 ug of whole cell lysate) A: hMSC-3A6 B: siRNA of ARHGEF2/GEF-H1 8% SDS PAGE diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577] - Paraffin-embedded rat hind brain. GEF-H1 antibody dilution: 1:500.

GEF-H1 antibody detects GEF-H1 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse mouse cerebellum.Green: GEF-H1 stained by GEF-H1 antibody (NBP2-21577) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500.Blue: Fluoroshield with DAPI . br>Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GEF-H1 antibody detects GEF-H1 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. GEF-H1 antibody (NBP2-21577) dilution: 1:500. br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (NBP2-21577) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (NBP2-21577) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

GEF-H1 antibody detects GEF-H1 protein by western blot analysis.A. 30 ug Neuro2A whole cell lysate/extractB. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw 264.7 whole cell lysate/extract G. 30 ug C2Cl2 whole cell lysate/extract5% SDS-PAGEGEF-H1 antibody (NBP2-21577) dilution: 1:1000The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ICC/IF, IHC, IP, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GEF-H1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit GEF-H1 Antibody - BSA Free (NBP2-21577) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. The exact sequence is proprietary.
• Localization: Cytoplasm; Cell junction; tight junction; Golgi apparatus; Spindle
• Predicted Species: Porcine (96%), Bovine (94%), Canine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARHGEF2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 112 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for GEF-H1 Antibody - BSA Free

• GEF
• GEFH1
• GEF-H1DKFZp547P1516
• Guanine nucleotide exchange factor H1
• KIAA0651DKFZp547L106
• LFP40FLJ45440
• Microtubule-regulated Rho-GEF
• P40
• Proliferating cell nucleolar antigen p40
• rho guanine nucleotide exchange factor 2
• Rho/Rac guanine nucleotide exchange factor (GEF) 2
• Rho/Rac guanine nucleotide exchange factor 2

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARHGEF2