GEF-H1 Antibody

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Western Blot: GEF-H1 Antibody [NBP2-21577] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: GEF-H1 Antibody [NBP2-21577] - HeLa cells were fixed in -20C 100% MeOH for 5 min. Green: ARHGEF2 protein stained by GEF-H1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Frozen: GEF-H1 Antibody [NBP2-21577] - Frozen-sectioned mouse mouse cerebellum. Green: GEF-H1 stained by GEF-H1 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at ...read more
Western Blot: GEF-H1 Antibody [NBP2-21577] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 5% SDS PAGE gel, diluted at 1:2000.
Western Blot: GEF-H1 Antibody [NBP2-21577] - A. 30 ug Raw264. 7 whole cell lysate/extract B. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody dilution: 1:1000. (Mouse)
Genetic Strategies: Western Blot: GEF-H1 Antibody [NBP2-21577] - Sample (10 ug of whole cell lysate) A: hMSC-3A6 B: siRNA of ARHGEF2/GEF-H1 8% SDS PAGE diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Western Blot: GEF-H1 Antibody [NBP2-21577] - Immunoprecipitation of GEF-H1 protein from 293T whole cell extracts using 5 ug of GEF-H1 antibody. Western blot analysis was performed using GEF-H1 antibody. EasyBlot ...read more
Western Blot: GEF-H1 Antibody [NBP2-21577] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract5% SDS-PAGE ...read more
Western Blot: GEF-H1 Antibody [NBP2-21577] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ...read more
Western Blot: GEF-H1 Antibody [NBP2-21577] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract5% SDS-PAGE ...read more
Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577] - Paraffin-embedded rat hind brain. GEF-H1 antibody dilution: 1:500.
Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577] - Paraffin-embedded mouse testis. GEF-H1 antibody dilution: 1:500.
Immunohistochemistry-Paraffin: GEF-H1 Antibody [NBP2-21577] - mouse brain. GEF-H1 stained by GEF-H1 antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

GEF-H1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. The exact sequence is proprietary.
Localization
Cytoplasm; Cell junction; tight junction; Golgi apparatus; Spindle
Predicted Species
Porcine (96%), Bovine (94%), Canine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARHGEF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
Theoretical MW
112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
GEF-H1 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for GEF-H1 Antibody

  • GEF
  • GEFH1
  • GEF-H1DKFZp547P1516
  • Guanine nucleotide exchange factor H1
  • KIAA0651DKFZp547L106
  • LFP40FLJ45440
  • Microtubule-regulated Rho-GEF
  • P40
  • Proliferating cell nucleolar antigen p40
  • rho guanine nucleotide exchange factor 2
  • Rho/Rac guanine nucleotide exchange factor (GEF) 2
  • Rho/Rac guanine nucleotide exchange factor 2

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GEF-H1 Antibody (NBP2-21577) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARHGEF2