Recombinant Human IL-22 Protein


1 µg/lane of Recombinant Human IL‑22 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human IL-22 (Catalog # 782‑IL) induces IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. The ED50 for this effect is 60‑300 pg/mL.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human IL-22 Protein Summary

Details of Functionality
Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. Nagalakshmi, M.L. et al. (2004) International Immunopharmacology 4:679. The ED50 for this effect is 60-300 pg/mL.
E. coli-derived human IL-22 protein
Ala34-Ile179, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
16.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
782-IL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-22 Protein

  • Cytokine Zcyto18
  • IL-10-related T-cell-derived inducible factor
  • IL-21
  • IL22
  • IL-22
  • IL-22IL21
  • IL-D110
  • IL-TIF
  • ILTIFIL-10-related T-cell-derived-inducible factor
  • IL-TIFMGC79382
  • interleukin 21
  • interleukin 22
  • interleukin-22
  • MGC79384
  • TIFa
  • TIFIL-23
  • zcyto18


Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT‑1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

  1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
  2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2000) PNAS 97:10144.
  4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Mk, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
Species: Hu
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
Species: Mu
Applications: WB, Block, CyTOF-ready, ELISA(Cap), ELISA(Det), ICFlow, ELISA(Sta)
Species: Hu
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for IL-22 (782-IL)(51)

We have publications tested in 4 confirmed species: Human, Mouse, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 5 applications: Bioassay, ELISA (Capture), ELISA (Standard), Surface Plasmon Resonance, Western Blot.

Filter By Application
ELISA (Capture)
ELISA (Standard)
Surface Plasmon Resonance
Western Blot
All Applications
Filter By Species
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
All Species
Showing Publications 1 - 10 of 51. Show All 51 Publications.
Publications using 782-IL Applications Species
S Sun, X Zhang, M Xu, F Zhang, F Tian, J Cui, Y Xia, C Liang, S Zhou, H Wei, H Zhao, G Wu, B Xu, X Liu, G Yang, Q Wang, L Zhang, Y Gong, C Shao, Y Zou Berberine downregulates CDC6 and inhibits proliferation via targeting JAK-STAT3 signaling in keratinocytes Cell Death Dis, 2019;10(4):274. 2019 [PMID: 30894513] (Bioassay, Human) Bioassay Human
F Abdallah, G Lecellier, P Raharivelo, C Pichon R. nukuhivensis acts by reinforcing skin barrier function, boosting skin immunity and by inhibiting IL-22 induced keratinocyte hyperproliferation Sci Rep, 2019;9(1):4132. 2019 [PMID: 30858525] (Bioassay, Human) Bioassay Human
JE Heath, GM Scholz, PD Veith, EC Reynolds IL-36? regulates mediators of tissue homeostasis in epithelial cells Cytokine, 2019;119(0):24-31. 2019 [PMID: 30856602] (Bioassay, Human) Bioassay Human
L Mercurio, M Morelli, C Scarponi, EZ Eisenmesse, N Doti, G Pagnanelli, E Gubinelli, C Mazzanti, A Cavani, M Ruvo, CA Dinarello, C Albanesi, S Madonna IL-38 has an anti-inflammatory action in psoriasis and its expression correlates with disease severity and therapeutic response to anti-IL-17A treatment Cell Death Dis, 2018;9(11):1104. 2018 [PMID: 30377293] (Bioassay, Human) Bioassay Human
M Abikhair B, N Roudiani, J Chen, AL Santana, N Doudican, C Proby, D Felsen, JA Carucci Ruxolitinib inhibits cyclosporine-induced proliferation of cutaneous squamous cell carcinoma JCI Insight, 2018;3(17):. 2018 [PMID: 30185657] (Bioassay, Human) Bioassay Human
S Nagel, C Pommerenke, C Meyer, M Kaufmann, RAF MacLeod, HG Drexler Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma Oncotarget, 2018;9(18):14338-14353. 2018 [PMID: 29581848] (Bioassay, Human) Bioassay Human
S Sawamura, M Jinnin, K Inoue, K Yamane, N Honda, I Kajihara, T Makino, S Masuguchi, S Fukushima, H Ihn Regulatory mechanisms of collagen expression by interleukin-22 signaling in scleroderma fibroblasts J. Dermatol. Sci., 2017;0(0):. 2017 [PMID: 29336866] (Bioassay, Human) Bioassay Human
S Abadpour, B Halvorsen, A Sahraoui, O Korsgren, P Aukrust, H Scholz Interleukin-22 reverses human islet dysfunction and apoptosis triggered by hyperglycemia and LIGHT J. Mol. Endocrinol., 2018;0(0):. 2018 [PMID: 29330151] (Bioassay, Human) Bioassay Human
R Bichele, J Kärner, K Truusalu, I Smidt, R Mändar, HR Conti, SL Gaffen, P Peterson, M Laan, K Kisand IL-22 neutralizing autoantibodies impair fungal clearance in murine oropharyngeal candidiasis model Eur. J. Immunol., 2017;0(0):. 2017 [PMID: 29150834] (Bioassay, Mouse) Bioassay Mouse
Y Wang, JB Mumm, R Herbst, R Kolbeck, Y Wang IL-22 Increases Permeability of Intestinal Epithelial Tight Junctions by Enhancing Claudin-2 Expression J. Immunol., 2017;0(0):. 2017 [PMID: 28939759] (Bioassay, Human) Bioassay Human
Show All 51 Publications.

Reviews for IL-22 (782-IL) (0)

There are no reviews for IL-22 (782-IL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-22 (782-IL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-22 Products

Bioinformatics Tool for IL-22 (782-IL)

Discover related pathways, diseases and genes to IL-22 (782-IL). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-22 (782-IL)

Discover more about diseases related to IL-22 (782-IL).

Pathways for IL-22 (782-IL)

View related products by pathway.

PTMs for IL-22 (782-IL)

Learn more about PTMs related to IL-22 (782-IL).

Blogs on IL-22.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

Customers Who Bought This Also Bought

CD4 Antibody

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human IL-22 Protein and receive a gift card or discount.


Gene Symbol IL22