IL-23A/IL-23 P19 Antibody - BSA Free

Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-76697] - Western Blot Validation in Mouse Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: IL-23A/IL-23 P19 NBP1-76697 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry-Paraffin: IL-23A/IL-23 P19 Antibody [NBP1-76697] - Mouse pancreas tissue with IL23 P19 antibody at 2 ug/ml.

Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-76697] - Mouse pancreas tissue lysate with IL-23 P19antibody at (A) 1 and (B) 2 ug/ml.

Western Blot: IL-23A/IL-23 P19 Antibody [NBP1-76697] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-23 recombinant protein per lane. Antibodies: IL-23A/IL-23 P19 NBP1-76697 (1: 0.25 ug/mL, 2: 0.5 ug/mL, 3: 1 ug/mL and 4: 2ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: IL-23A/IL-23 P19 Antibody - BSA Free [NBP1-76697] - Validation of IL-23A/IL-23 P19 in Mouse Pancreas Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas tissue labeling IL-23A/IL-23 P19 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

• Reactivity: Hu, Mu, Eq
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IL-23A/IL-23 P19 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IL-23A/IL-23 P19 Antibody - BSA Free (NBP1-76697) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human IL-23. The immunogen is located within amino acids 40 - 90 of IL-23. Amino Acid Squence: REEGDEETTNDVPHIQC
• Predicted Species: Equine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL23A
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 0.25-2 ug/ml
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IL-23A/IL-23 P19 Peptide (NBP1-76697PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Pig (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IL-23A/IL-23 P19 Antibody - BSA Free

• IL-23 p19/IL-12 p40
• IL23
• IL-23
• IL-23A
• IL-23-A
• IL-23p19
• IL-23p19/IL-12p40
• IL23P19P19
• interleukin 23 p19 subunit
• interleukin 23, alpha subunit p19
• interleukin-23 subunit alpha
• Interleukin-23 subunit p19
• JKA3 induced upon T-cell activation
• MGC79388
• SGRF
• SGRFIL-23 subunit alpha

Background

Like interleukin-27 (IL-27), IL-23 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the IL-12p40 subunit and a novel p19 subunit. IL-23 is secreted by activated dendritic cells, macrophages, and monocytes. Its biological activities include enhancing the proliferation of memory T cells and the production of IFN-gamma, IL-12, and TNF-alpha from activated T cells, and can stimulate macrophages to produce TNF-alpha and nitric oxide. It has also been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-23A/IL-23 P19 Antibody (NBP1-76697)(1)

Publication using NBP1-76697

• Jeong, U;Lee, WH;Choi, YS;Haseeb, M;Baek, WY;Han, JH;Choi, H;Kim, MS;Suh, CH;Kim, W;Choi, S; Discovery of ETI41 and ETI60: novel selective endosomal Toll-like receptor inhibitors for the treatment of autoimmune diseases Experimental & molecular medicine 2025-09-01 [PMID: 40887497]

Bioinformatics

• Gene Symbol: IL23A
• Entrez:
  • 51561(Human)
  • 83430(Mouse)
• Uniprot:
  • Q9NPF7()