IL-17/IL-17A Antibody - Azide and BSA Free

Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis of rat recombinant IL-17A protein (lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG.

Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis using the Biotin conjugate of NBP1-42772. Detection of a band ~17 kDa in size corresponding to recombinant human IL17-A (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution.

Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis using the HRP conjugate of NBP1-42772. Detection of Lane 1: Recombinant Rat IL-17A. Load: 50 ng per lane. Used at a dilution of 1:1,000 o/n at 4C.

Western Blot: IL-17/IL-17A Antibody [NBP1-42772] - Analysis of mouse recombinant IL-17A protein (arrowhead, lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight 649 conjugated Gt-a-Rabbit IgG.

Western blot using anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight(TM) 649 conjugated Gt-a-Rabbit IgG

Western blot using anti-IL-17A antibody shows detection of rat recombinant IL-17A protein (arrowhead, lane 1). Approximately 2 ug of recombinant protein was loaded onto the gel. Primary antibody was used at a 1:1,000 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight(TM) 649 conjugated Gt-a-Rabbit IgG

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

IL-17/IL-17A Antibody - Azide and BSA Free Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This purified IL-17/IL-17A Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL-17/IL-17A protein. (Uniprot: Q61453)
• Specificity: In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant rat IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL17A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:5000
  • Western Blot 1:1000
• Application Notes: This purified antibody has been tested for use in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. The recombinant immunogen is a non-glycosylated homodimer joined by disulfide bonds having a molecular mass of 30.0 kDa. By western blot from a reducing gel expect a band approximately 15.0 kDa in size corresponding to rat IL17-A protein in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26356122).

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for IL-17/IL-17A Antibody - Azide and BSA Free

• CTLA8
• CTLA-8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• IL17
• IL-17
• IL17A
• IL-17A
• IL-17Acytotoxic T-lymphocyte-associated protein 8
• IL-17CTLA-8
• IL17interleukin-17A
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A

Background

Rat IL17-A (also known as Interleukin-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8) is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Rat IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58% identity between the amino acid sequence of human and rat IL-17A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17/IL-17A Antibody (NBP1-42772)(4)

Publications using NBP1-42772

• Iqbal H, Kim GL, Kim JH et al. Pep27 Mutant Immunization Inhibits Caspase-14 Expression to Alleviate Inflammatory Bowel Disease via Treg Upregulation Microorganisms 2022-09-19 [PMID: 36144473] (Human)
• Guo J, Peng L, Zeng J et al. Paeoniflorin suppresses allergic and inflammatory responses by promoting autophagy in rats with urticaria Exp Ther Med 2021-04-22 [PMID: 33884028] (IF/IHC, Rat)
  
  Details:
  Citation using the Biotin format of this antibody.
• Liu Y, Sun X, Zhao X et al. Expression and location of IL-17A, E, F and their receptors in colorectal adenocarcinoma: Comparison with benign intestinal disease Pathol Res Pract 2018-04-19 [PMID: 29548809] (Human)
  
  Details:
  Citation using the Biotin form of this antibody.
• Liu Y, Zhao X, Sun X et al. Expression of IL-17A, E, and F and their receptors in human prostatic cancer: Comparison with benign prostatic hyperplasia. Prostate. 2015-12-01 [PMID: 26356122] (Human)
  
  Details:
  This citation used the Biotin version of this antibody.

FAQs for IL-17/IL-17A Antibody (NBP1-42772). (Showing 1 - 2 of 2 FAQs).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
   • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Bioinformatics

• Gene Symbol: IL17A
• Uniprot:
  • Q61453(Rat)