Recombinant Human IL-23 Protein


Recombinant Human IL-23 (Catalog # 1290-IL) induces IL-17 secretion by mouse splenocytes. The ED50 is approximately 2-fold better than top competitor. The protein activity is significantly better with nearly twice the more

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human IL-23 Protein Summary

Details of Functionality
Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The ED50 for this effect is 0.05-0.3 ng/mL.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-23 protein
Human IL-23 p40
Accession # P29460
Accession # AAG37232
N-terminus C-terminus
Accession #
N-terminal Sequence
Ile23 (IL-23 single chain & p40) & Ala21 (p19)
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
55.0 kDa (IL-23 single chain); 36.5 kDa (p40) & 18.5 kDa (p19).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
65 kDa, 43-45 kDa and 20 kDa, reducing conditions
Read Publications using
1290-IL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in MES, NaCl and EDTA with BSA as a carrier protein.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-23 Protein

  • IL-23 p19/IL-12 p40
  • IL23
  • IL-23
  • IL-23A
  • IL-23-A
  • IL-23p19
  • IL-23p19/IL-12p40
  • IL23P19P19
  • interleukin 23 p19 subunit
  • interleukin 23, alpha subunit p19
  • interleukin-23 subunit alpha
  • Interleukin-23 subunit p19
  • JKA3 induced upon T-cell activation
  • MGC79388
  • SGRF
  • SGRFIL-23 subunit alpha


Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Human p19 cDNA encodes a 189 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 170 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5699.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Customers Who Viewed This Item Also Viewed...

Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
Species: Mu
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, IHC, CyTOF-ready, ICFlow
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for IL-23A/IL-23 P19 (1290-IL)(88)

We have publications tested in 4 confirmed species: Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 3 applications: Bioassay, Cell Culture, ELISA (Standard).

Filter By Application
Cell Culture
ELISA (Standard)
All Applications
Filter By Species
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca mulatta (Rhesus Macaque)
All Species
Showing Publications 1 - 10 of 88. Show All 88 Publications.
Publications using 1290-IL Applications Species
C Seillet, K Luong, J Tellier, N Jacquelot, RD Shen, P Hickey, VC Wimmer, L Whitehead, K Rogers, GK Smyth, AL Garnham, ME Ritchie, GT Belz The neuropeptide VIP confers anticipatory mucosal immunity by regulating ILC3 activity Nat. Immunol., 2019;0(0):. 2019 [PMID: 31873294] (Cell Culture, Mouse) Cell Culture Mouse
C Li, J Zhang, W Wang, H Wang, Y Zhang, Z Zhang Data on arsenic trioxide modulates Treg/Th17/Th1/Th2 cells in treatment-na�ve rheumatoid arthritis patients and collagen-induced arthritis model mice Data Brief, 2019;27(0):104615. 2019 [PMID: 31871961] (Bioassay, Human) Bioassay Human
JH Bernink, Y Ohne, MBM Teunissen, J Wang, J Wu, L Krabbendam, C Guntermann, R Volckmann, J Koster, S van Tol, I Ramirez, Y Shrestha, MA de Rie, H Spits, X Romero Ros, AA Humbles c-Kit-positive ILC2s exhibit an ILC3-like signature that may contribute to IL-17-mediated pathologies Nat. Immunol., 2019;0(0):. 2019 [PMID: 31263279] (Cell Culture, Human) Cell Culture Human
JZ Kawalkowsk, J Ogbechi, PJ Venables, RO Williams cIAP1/2 inhibition synergizes with TNF inhibition in autoimmunity by down-regulating IL-17A and inducing Tregs Sci Adv, 2019;5(5):eaaw5422. 2019 [PMID: 31049403] (Bioassay, Human) Bioassay Human
T Hochdörfer, C Winkler, K Pardali, J Mjösberg Expression of c-Kit discriminates between two functionally distinct subsets of human type 2 innate lymphoid cells Eur. J. Immunol., 2019;0(0):. 2019 [PMID: 30892687] (Bioassay, Human) Bioassay Human
KC Doh, BM Kim, KW Kim, BH Chung, CW Yang Effects of resveratrol on Th17 cell-related immune responses under tacrolimus-based immunosuppression BMC Complement Altern Med, 2019;19(1):54. 2019 [PMID: 30832648] (Bioassay, Human) Bioassay Human
HG Lee, JU Lee, DH Kim, S Lim, I Kang, JM Choi Pathogenic function of bystander-activated memory-like CD4+ T cells in autoimmune encephalomyelitis Nat Commun, 2019;10(1):709. 2019 [PMID: 30755603] (Bioassay, Human) Bioassay Human
L Chapuy, M Bsat, S Sarkizova, M Rubio, A Therrien, E Wassef, M Bouin, K Orlicka, A Weber, N Hacohen, AC Villani, M Sarfati Two distinct colonic CD14+ subsets characterized by single-cell RNA profiling in Crohn&#039;s disease Mucosal Immunol, 2019;0(0):. 2019 [PMID: 30670762] (Bioassay, Human) Bioassay Human
S Nagel, C Pommerenke, C Meyer, M Kaufmann, RAF MacLeod, HG Drexler Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma Oncotarget, 2018;9(18):14338-14353. 2018 [PMID: 29581848] (Bioassay, Human) Bioassay Human
X Xu, C Yang, J Chen, J Liu, P Li, Y Shi, P Yu Interleukin-23 promotes the migration and invasion of gastric cancer cells by inducing epithelial-to-mesenchymal transition via the STAT3 pathway Biochem. Biophys. Res. Commun., 2018;0(0):. 2018 [PMID: 29574157]
Show All 88 Publications.

Reviews for IL-23A/IL-23 P19 (1290-IL) (0)

There are no reviews for IL-23A/IL-23 P19 (1290-IL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-23A/IL-23 P19 (1290-IL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional IL-23A/IL-23 P19 Products

Bioinformatics Tool for IL-23A/IL-23 P19 (1290-IL)

Discover related pathways, diseases and genes to IL-23A/IL-23 P19 (1290-IL). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-23A/IL-23 P19 (1290-IL)

Discover more about diseases related to IL-23A/IL-23 P19 (1290-IL).

Pathways for IL-23A/IL-23 P19 (1290-IL)

View related products by pathway.

PTMs for IL-23A/IL-23 P19 (1290-IL)

Learn more about PTMs related to IL-23A/IL-23 P19 (1290-IL).

Blogs on IL-23A/IL-23 P19

There are no specific blogs for IL-23A/IL-23 P19, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human IL-23 Protein and receive a gift card or discount.


Gene Symbol IL23A