IL-17RA/IL-17R Antibody (133617) [Unconjugated]

Images

 
Recombinant Human IL‑17/IL‑17A induces CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GRO alpha DuoSet ...read more
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 R Monoclonal Antibody (Catalog # MAB177, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) ...read more
Genetic Strategies: IL-17 RA/IL-17 R knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB177, filled histogram) or isotype ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready, KO
Clone
133617
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

IL-17RA/IL-17R Antibody (133617) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑17 RA/IL‑17 R
Leu33-Trp320
Accession # Q96F46
Specificity
Detects human IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed
Details of Functionality
ActivityAssaywCitation not found None None None None
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL17RA
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Knockout Validated 0.25 ug/10^6 cells
  • Neutralization
Publications
Read Publications using
MAB177 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17RA/IL-17R Antibody (133617) [Unconjugated]

  • CD217 antigen
  • CD217
  • Cdw217
  • CDw217interleukin 17 receptor
  • hIL-17R
  • IL-17 R
  • IL-17 RA
  • IL-17 receptor A
  • IL17RA
  • IL-17RA
  • IL-17RAMGC10262
  • IL17Rinterleukin-17 receptor A
  • interleukin 17 receptor A

Background

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL‑17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.

  1. Yao, Z. et al. (1997) Cytokine 9:794.
  2. Schwander, R. et al. (2000) J. Exp. Med. 191:1233.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-17RA/IL-17R Antibody (MAB177)(13)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow Cytometry, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(3)
Neutralization
(7)
Western Blot
(3)
All Applications
Filter By Species
Human
(13)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using MAB177 Applications Species
J Wu, J Guo, Q Cao, Y Wang, J Chen, Z Wang, Z Yuan Autophagy impacts on oxaliplatin-induced hepatocarcinoma apoptosis via the IL-17/IL-17R-JAK2/STAT3 signaling pathway Oncol Lett, 2017;13(2):770-776. 2017 [PMID: 28356957] (Neutralization, Human) Neutralization Human
PA Szabo, A Goswami, DM Mazzuca, K Kim, DB O'Gorman, DA Hess, ID Welch, HA Young, B Singh, JK McCormick, SM Haeryfar Rapid and Rigorous IL-17A Production by a Distinct Subpopulation of Effector Memory T Lymphocytes Constitutes a Novel Mechanism of Toxic Shock Syndrome Immunopathology J. Immunol, 2017;0(0):. 2017 [PMID: 28219889] (Neutralization, Human) Neutralization Human
Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage. Eur J Immunol, 2015;46(2):440-5. 2015 [PMID: 26558442] (Western Blot, Human) Western Blot Human
Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients. Mucosal Immunol, 0;5(2):140-9. 0 [PMID: 22189956] (Flow Cytometry, Human) Flow Cytometry Human
Ramirez-Carrozzi V, Sambandam A, Luis E IL-17C regulates the innate immune function of epithelial cells in an autocrine manner. Nat. Immunol., 2011;12(12):1159-66. 2011 [PMID: 21993848] (Neutralization, Human) Neutralization Human
Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH TH17 cytokines induce human airway smooth muscle cell migration. J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2. 2011 [PMID: 21345484] (Neutralization, Human) Neutralization Human
Corrigan CJ, Wang W, Meng Q, Fang C, Wu H, Reay V, Lv Z, Fan Y, An Y, Wang YH, Liu YJ, Lee TH, Ying S T-helper cell type 2 (Th2) memory T cell-potentiating cytokine IL-25 has the potential to promote angiogenesis in asthma. Proc. Natl. Acad. Sci. U.S.A., 2011;108(4):1579-84. 2011 [PMID: 21205894] (Neutralization, Human) Neutralization Human
Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin&#039;s lymphoma. Int. J. Cancer, 2009;125(5):1092-101. 2009 [PMID: 19391137] (Western Blot, Human) Western Blot Human
Shahrara S, Pickens SR, Dorfleutner A, Pope RM IL-17 induces monocyte migration in rheumatoid arthritis. J. Immunol., 2009;182(6):3884-91. 2009 [PMID: 19265168] (Neutralization, Human) Neutralization Human
Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Circulation, 2009;119(10):1424-32. 2009 [PMID: 19255340] (Neutralization, Human) Neutralization Human
Show All 13 Publications.

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Bioinformatics

Gene Symbol IL17RA
Uniprot